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Optical Stimulation of Zebrafish Hair Cells Expressing Channelrhodopsin-2
Vertebrate hair cells are responsible for the high fidelity encoding of mechanical stimuli into trains of action potentials (spikes) in afferent neurons. Here, we generated a transgenic zebrafish line expressing Channelrhodopsin-2 (ChR2) under the control of the hair-cell specific myo6b promoter, in...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4008597/ https://www.ncbi.nlm.nih.gov/pubmed/24791934 http://dx.doi.org/10.1371/journal.pone.0096641 |
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author | Monesson-Olson, Bryan D. Browning-Kamins, Jenna Aziz-Bose, Razina Kreines, Fabiana Trapani, Josef G. |
author_facet | Monesson-Olson, Bryan D. Browning-Kamins, Jenna Aziz-Bose, Razina Kreines, Fabiana Trapani, Josef G. |
author_sort | Monesson-Olson, Bryan D. |
collection | PubMed |
description | Vertebrate hair cells are responsible for the high fidelity encoding of mechanical stimuli into trains of action potentials (spikes) in afferent neurons. Here, we generated a transgenic zebrafish line expressing Channelrhodopsin-2 (ChR2) under the control of the hair-cell specific myo6b promoter, in order to examine the role of the mechanoelectrical transduction (MET) channel in sensory encoding in afferent neurons. We performed in vivo recordings from afferent neurons of the zebrafish lateral line while activating hair cells with either mechanical stimuli from a waterjet or optical stimuli from flashes of ∼470-nm light. Comparison of the patterns of encoded spikes during 100-ms stimuli revealed no difference in mean first spike latency between the two modes of activation. However, there was a significant increase in the variability of first spike latency during optical stimulation as well as an increase in the mean number of spikes per stimulus. Next, we compared encoding of spikes during hair-cell stimulation at 10, 20, and 40-Hz. Consistent with the increased variability of first spike latency, we saw a significant decrease in the vector strength of phase-locked spiking during optical stimulation. These in vivo results support a physiological role for the MET channel in the high fidelity of first spike latency seen during encoding of mechanical sensory stimuli. Finally, we examined whether remote activation of hair cells via ChR2 activation was sufficient to elicit escape responses in free-swimming larvae. In transgenic larvae, 100-ms flashes of ∼470-nm light resulted in escape responses that occurred concomitantly with field recordings indicating Mauthner cell activity. Altogether, the myo6b:ChR2 transgenic line provides a platform to investigate hair-cell function and sensory encoding, hair-cell sensory input to the Mauthner cell, and the ability to remotely evoke behavior in free-swimming zebrafish. |
format | Online Article Text |
id | pubmed-4008597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40085972014-05-09 Optical Stimulation of Zebrafish Hair Cells Expressing Channelrhodopsin-2 Monesson-Olson, Bryan D. Browning-Kamins, Jenna Aziz-Bose, Razina Kreines, Fabiana Trapani, Josef G. PLoS One Research Article Vertebrate hair cells are responsible for the high fidelity encoding of mechanical stimuli into trains of action potentials (spikes) in afferent neurons. Here, we generated a transgenic zebrafish line expressing Channelrhodopsin-2 (ChR2) under the control of the hair-cell specific myo6b promoter, in order to examine the role of the mechanoelectrical transduction (MET) channel in sensory encoding in afferent neurons. We performed in vivo recordings from afferent neurons of the zebrafish lateral line while activating hair cells with either mechanical stimuli from a waterjet or optical stimuli from flashes of ∼470-nm light. Comparison of the patterns of encoded spikes during 100-ms stimuli revealed no difference in mean first spike latency between the two modes of activation. However, there was a significant increase in the variability of first spike latency during optical stimulation as well as an increase in the mean number of spikes per stimulus. Next, we compared encoding of spikes during hair-cell stimulation at 10, 20, and 40-Hz. Consistent with the increased variability of first spike latency, we saw a significant decrease in the vector strength of phase-locked spiking during optical stimulation. These in vivo results support a physiological role for the MET channel in the high fidelity of first spike latency seen during encoding of mechanical sensory stimuli. Finally, we examined whether remote activation of hair cells via ChR2 activation was sufficient to elicit escape responses in free-swimming larvae. In transgenic larvae, 100-ms flashes of ∼470-nm light resulted in escape responses that occurred concomitantly with field recordings indicating Mauthner cell activity. Altogether, the myo6b:ChR2 transgenic line provides a platform to investigate hair-cell function and sensory encoding, hair-cell sensory input to the Mauthner cell, and the ability to remotely evoke behavior in free-swimming zebrafish. Public Library of Science 2014-05-02 /pmc/articles/PMC4008597/ /pubmed/24791934 http://dx.doi.org/10.1371/journal.pone.0096641 Text en © 2014 Monesson-Olson et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Monesson-Olson, Bryan D. Browning-Kamins, Jenna Aziz-Bose, Razina Kreines, Fabiana Trapani, Josef G. Optical Stimulation of Zebrafish Hair Cells Expressing Channelrhodopsin-2 |
title | Optical Stimulation of Zebrafish Hair Cells Expressing Channelrhodopsin-2 |
title_full | Optical Stimulation of Zebrafish Hair Cells Expressing Channelrhodopsin-2 |
title_fullStr | Optical Stimulation of Zebrafish Hair Cells Expressing Channelrhodopsin-2 |
title_full_unstemmed | Optical Stimulation of Zebrafish Hair Cells Expressing Channelrhodopsin-2 |
title_short | Optical Stimulation of Zebrafish Hair Cells Expressing Channelrhodopsin-2 |
title_sort | optical stimulation of zebrafish hair cells expressing channelrhodopsin-2 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4008597/ https://www.ncbi.nlm.nih.gov/pubmed/24791934 http://dx.doi.org/10.1371/journal.pone.0096641 |
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