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Comparison of a New Gold Immunochromatographic Assay for the Rapid Diagnosis of the Novel Influenza A (H7N9) Virus with Cell Culture and a Real-Time Reverse-Transcription PCR Assay

We assessed a colloidal gold immunochromatographic assay (GICA) for rapid detection of influenza A (H7N9) and compared it with reverse-transcription-polymerase chain reaction (RT-PCR) and viral culture. Samples from 35 H7N9 infected patients were collected, including 45 throat swab samples, 56 sputu...

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Autores principales: Jin, Changzhong, Wu, Nanping, Peng, Xiaorong, Yao, Hangping, Lu, Xiangyun, Chen, Yu, Wu, Haibo, Xie, Tiansheng, Cheng, Linfang, Liu, Fumin, Kang, Keren, Tang, Shixing, Li, Lanjuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009114/
https://www.ncbi.nlm.nih.gov/pubmed/24822207
http://dx.doi.org/10.1155/2014/425051
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author Jin, Changzhong
Wu, Nanping
Peng, Xiaorong
Yao, Hangping
Lu, Xiangyun
Chen, Yu
Wu, Haibo
Xie, Tiansheng
Cheng, Linfang
Liu, Fumin
Kang, Keren
Tang, Shixing
Li, Lanjuan
author_facet Jin, Changzhong
Wu, Nanping
Peng, Xiaorong
Yao, Hangping
Lu, Xiangyun
Chen, Yu
Wu, Haibo
Xie, Tiansheng
Cheng, Linfang
Liu, Fumin
Kang, Keren
Tang, Shixing
Li, Lanjuan
author_sort Jin, Changzhong
collection PubMed
description We assessed a colloidal gold immunochromatographic assay (GICA) for rapid detection of influenza A (H7N9) and compared it with reverse-transcription-polymerase chain reaction (RT-PCR) and viral culture. Samples from 35 H7N9 infected patients were collected, including 45 throat swab samples, 56 sputum samples, and 39 feces samples. All samples were tested by GICA, viral culture, and RT-PCR. GICA specifically reacted with recombinant HA proteins, virus lysates, and clinical samples from H7 subtype viruses. Compared with RT-PCR, GICA demonstrated low sensitivity (33.33%) but high specificity (97.56%). The positive rate of GICA tests for samples collected in the period from 8 to 21 days after contact with poultry was much higher than those for samples collected before or after this period. Compared with viral culture, GICA showed sensitivity of 91.67% and specificity of 82.03%. Sputum specimens were more likely to test positive for H7N9 virus than samples from throat swabs and feces. The GICA-based H7 test is a reliable, rapid, and convenient method for the screening and diagnosis of influenza A (H7N9) disease, especially for the sputum specimens with high viral load. It may be helpful in managing H7N9 epidemics and preliminary diagnosis in early stages in resource-limited settings.
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spelling pubmed-40091142014-05-12 Comparison of a New Gold Immunochromatographic Assay for the Rapid Diagnosis of the Novel Influenza A (H7N9) Virus with Cell Culture and a Real-Time Reverse-Transcription PCR Assay Jin, Changzhong Wu, Nanping Peng, Xiaorong Yao, Hangping Lu, Xiangyun Chen, Yu Wu, Haibo Xie, Tiansheng Cheng, Linfang Liu, Fumin Kang, Keren Tang, Shixing Li, Lanjuan Biomed Res Int Research Article We assessed a colloidal gold immunochromatographic assay (GICA) for rapid detection of influenza A (H7N9) and compared it with reverse-transcription-polymerase chain reaction (RT-PCR) and viral culture. Samples from 35 H7N9 infected patients were collected, including 45 throat swab samples, 56 sputum samples, and 39 feces samples. All samples were tested by GICA, viral culture, and RT-PCR. GICA specifically reacted with recombinant HA proteins, virus lysates, and clinical samples from H7 subtype viruses. Compared with RT-PCR, GICA demonstrated low sensitivity (33.33%) but high specificity (97.56%). The positive rate of GICA tests for samples collected in the period from 8 to 21 days after contact with poultry was much higher than those for samples collected before or after this period. Compared with viral culture, GICA showed sensitivity of 91.67% and specificity of 82.03%. Sputum specimens were more likely to test positive for H7N9 virus than samples from throat swabs and feces. The GICA-based H7 test is a reliable, rapid, and convenient method for the screening and diagnosis of influenza A (H7N9) disease, especially for the sputum specimens with high viral load. It may be helpful in managing H7N9 epidemics and preliminary diagnosis in early stages in resource-limited settings. Hindawi Publishing Corporation 2014 2014-04-14 /pmc/articles/PMC4009114/ /pubmed/24822207 http://dx.doi.org/10.1155/2014/425051 Text en Copyright © 2014 Changzhong Jin et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jin, Changzhong
Wu, Nanping
Peng, Xiaorong
Yao, Hangping
Lu, Xiangyun
Chen, Yu
Wu, Haibo
Xie, Tiansheng
Cheng, Linfang
Liu, Fumin
Kang, Keren
Tang, Shixing
Li, Lanjuan
Comparison of a New Gold Immunochromatographic Assay for the Rapid Diagnosis of the Novel Influenza A (H7N9) Virus with Cell Culture and a Real-Time Reverse-Transcription PCR Assay
title Comparison of a New Gold Immunochromatographic Assay for the Rapid Diagnosis of the Novel Influenza A (H7N9) Virus with Cell Culture and a Real-Time Reverse-Transcription PCR Assay
title_full Comparison of a New Gold Immunochromatographic Assay for the Rapid Diagnosis of the Novel Influenza A (H7N9) Virus with Cell Culture and a Real-Time Reverse-Transcription PCR Assay
title_fullStr Comparison of a New Gold Immunochromatographic Assay for the Rapid Diagnosis of the Novel Influenza A (H7N9) Virus with Cell Culture and a Real-Time Reverse-Transcription PCR Assay
title_full_unstemmed Comparison of a New Gold Immunochromatographic Assay for the Rapid Diagnosis of the Novel Influenza A (H7N9) Virus with Cell Culture and a Real-Time Reverse-Transcription PCR Assay
title_short Comparison of a New Gold Immunochromatographic Assay for the Rapid Diagnosis of the Novel Influenza A (H7N9) Virus with Cell Culture and a Real-Time Reverse-Transcription PCR Assay
title_sort comparison of a new gold immunochromatographic assay for the rapid diagnosis of the novel influenza a (h7n9) virus with cell culture and a real-time reverse-transcription pcr assay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009114/
https://www.ncbi.nlm.nih.gov/pubmed/24822207
http://dx.doi.org/10.1155/2014/425051
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