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Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy

We analyzed embryo culture medium (CM) and recipient blood plasma using Fourier transform infrared spectroscopy (FTIR) metabolomics to identify spectral models predictive of pregnancy outcome. Embryos collected on Day 6 from superovulated cows in 2 countries were individually cultured in synthetic o...

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Autores principales: Muñoz, Marta, Uyar, Asli, Correia, Eva, Ponsart, Claire, Guyader-Joly, Catherine, Martínez-Bello, Daniel, Marquant-Le Guienne, Brigitte, Fernandez-Gonzalez, Alfonso, Díez, Carmen, Caamaño, Jose Nestor, Trigal, Beatriz, Humblot, Patrice, Carrocera, Susana, Martin, David, Seli, Emre, Gomez, Enrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009133/
https://www.ncbi.nlm.nih.gov/pubmed/24834432
http://dx.doi.org/10.1155/2014/608579
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author Muñoz, Marta
Uyar, Asli
Correia, Eva
Ponsart, Claire
Guyader-Joly, Catherine
Martínez-Bello, Daniel
Marquant-Le Guienne, Brigitte
Fernandez-Gonzalez, Alfonso
Díez, Carmen
Caamaño, Jose Nestor
Trigal, Beatriz
Humblot, Patrice
Carrocera, Susana
Martin, David
Seli, Emre
Gomez, Enrique
author_facet Muñoz, Marta
Uyar, Asli
Correia, Eva
Ponsart, Claire
Guyader-Joly, Catherine
Martínez-Bello, Daniel
Marquant-Le Guienne, Brigitte
Fernandez-Gonzalez, Alfonso
Díez, Carmen
Caamaño, Jose Nestor
Trigal, Beatriz
Humblot, Patrice
Carrocera, Susana
Martin, David
Seli, Emre
Gomez, Enrique
author_sort Muñoz, Marta
collection PubMed
description We analyzed embryo culture medium (CM) and recipient blood plasma using Fourier transform infrared spectroscopy (FTIR) metabolomics to identify spectral models predictive of pregnancy outcome. Embryos collected on Day 6 from superovulated cows in 2 countries were individually cultured in synthetic oviduct fluid medium with BSA for 24 h before embryo transfer. Spent CM, blank controls, and plasma samples (Day 0 and Day 7) were evaluated using FTIR. The spectra obtained were analyzed. The discrimination capability of the classifiers was assessed for accuracy, sensitivity (pregnancy), specificity (nonpregnancy), and area under the ROC curve (AUC). Endpoints considered were Day 60 pregnancy and birth. High AUC was obtained for Day 60 pregnancy in CM within individual laboratories (France AUC = 0.751 ± 0.039, Spain AUC = 0.718 ± 0.024), while cumulative data decreased the AUC (AUC = 0.604  ±  0.029). Predictions for CM at birth were lower than Day 60 pregnancy. Predictions with plasma at birth improved cumulative over individual results (Day 0: France AUC = 0.690 ± 0.044; Spain AUC < 0.55; cumulative AUC = 0.747 ± 0.032). Plasma generally predicted pregnancy and birth better than CM. These first results show that FTIR metabolomics could allow the identification of embryos and recipients with improved pregnancy viability, which may contribute to increasing the efficiency of selection schemes based on ET.
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spelling pubmed-40091332014-05-15 Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy Muñoz, Marta Uyar, Asli Correia, Eva Ponsart, Claire Guyader-Joly, Catherine Martínez-Bello, Daniel Marquant-Le Guienne, Brigitte Fernandez-Gonzalez, Alfonso Díez, Carmen Caamaño, Jose Nestor Trigal, Beatriz Humblot, Patrice Carrocera, Susana Martin, David Seli, Emre Gomez, Enrique Biomed Res Int Research Article We analyzed embryo culture medium (CM) and recipient blood plasma using Fourier transform infrared spectroscopy (FTIR) metabolomics to identify spectral models predictive of pregnancy outcome. Embryos collected on Day 6 from superovulated cows in 2 countries were individually cultured in synthetic oviduct fluid medium with BSA for 24 h before embryo transfer. Spent CM, blank controls, and plasma samples (Day 0 and Day 7) were evaluated using FTIR. The spectra obtained were analyzed. The discrimination capability of the classifiers was assessed for accuracy, sensitivity (pregnancy), specificity (nonpregnancy), and area under the ROC curve (AUC). Endpoints considered were Day 60 pregnancy and birth. High AUC was obtained for Day 60 pregnancy in CM within individual laboratories (France AUC = 0.751 ± 0.039, Spain AUC = 0.718 ± 0.024), while cumulative data decreased the AUC (AUC = 0.604  ±  0.029). Predictions for CM at birth were lower than Day 60 pregnancy. Predictions with plasma at birth improved cumulative over individual results (Day 0: France AUC = 0.690 ± 0.044; Spain AUC < 0.55; cumulative AUC = 0.747 ± 0.032). Plasma generally predicted pregnancy and birth better than CM. These first results show that FTIR metabolomics could allow the identification of embryos and recipients with improved pregnancy viability, which may contribute to increasing the efficiency of selection schemes based on ET. Hindawi Publishing Corporation 2014 2014-04-15 /pmc/articles/PMC4009133/ /pubmed/24834432 http://dx.doi.org/10.1155/2014/608579 Text en Copyright © 2014 Marta Muñoz et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Muñoz, Marta
Uyar, Asli
Correia, Eva
Ponsart, Claire
Guyader-Joly, Catherine
Martínez-Bello, Daniel
Marquant-Le Guienne, Brigitte
Fernandez-Gonzalez, Alfonso
Díez, Carmen
Caamaño, Jose Nestor
Trigal, Beatriz
Humblot, Patrice
Carrocera, Susana
Martin, David
Seli, Emre
Gomez, Enrique
Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy
title Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy
title_full Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy
title_fullStr Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy
title_full_unstemmed Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy
title_short Metabolomic Prediction of Pregnancy Viability in Superovulated Cattle Embryos and Recipients with Fourier Transform Infrared Spectroscopy
title_sort metabolomic prediction of pregnancy viability in superovulated cattle embryos and recipients with fourier transform infrared spectroscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009133/
https://www.ncbi.nlm.nih.gov/pubmed/24834432
http://dx.doi.org/10.1155/2014/608579
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