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Differential Transcript Profiles of MHC Class Ib(Qa-1, Qa-2, and Qa-10) and Aire Genes during the Ontogeny of Thymus and Other Tissues

Qa-2 and Qa-1 are murine nonclassical MHC class I molecules involved in the modulation of immune responses by interacting with T CD8(+) and NK cell inhibitory receptors. During thymic education, the Aire gene imposes the expression of thousands of tissue-related antigens in the thymic medulla, permi...

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Autores principales: Melo-Lima, Breno Luiz, Evangelista, Adriane Feijó, de Magalhães, Danielle Aparecida Rosa, Passos, Geraldo Aleixo, Moreau, Philippe, Donadi, Eduardo Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009201/
https://www.ncbi.nlm.nih.gov/pubmed/24829926
http://dx.doi.org/10.1155/2014/159247
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author Melo-Lima, Breno Luiz
Evangelista, Adriane Feijó
de Magalhães, Danielle Aparecida Rosa
Passos, Geraldo Aleixo
Moreau, Philippe
Donadi, Eduardo Antonio
author_facet Melo-Lima, Breno Luiz
Evangelista, Adriane Feijó
de Magalhães, Danielle Aparecida Rosa
Passos, Geraldo Aleixo
Moreau, Philippe
Donadi, Eduardo Antonio
author_sort Melo-Lima, Breno Luiz
collection PubMed
description Qa-2 and Qa-1 are murine nonclassical MHC class I molecules involved in the modulation of immune responses by interacting with T CD8(+) and NK cell inhibitory receptors. During thymic education, the Aire gene imposes the expression of thousands of tissue-related antigens in the thymic medulla, permitting the negative selection events. Aiming to characterize the transcriptional profiles of nonclassical MHC class I genes in spatial-temporal association with the Aire expression, we evaluated the gene expression of H2-Q7(Qa-2), H2-T23(Qa-1), H2-Q10(Qa-10), and Aire during fetal and postnatal development of thymus and other tissues. In the thymus, H2-Q7(Qa-2) transcripts were detected at high levels throughout development and were positively correlated with Aire expression during fetal ages. H2-Q7(Qa-2) and H2-T23(Qa-1) showed distinct expression patterns with gradual increasing levels according to age in most tissues analyzed. H2-Q10(Qa-10) was preferentially expressed by the liver. The Aire transcriptional profile showed increased levels during the fetal period and was detectable in postnatal ages in the thymus. Overall, nonclassical MHC class I genes started to be expressed early during the ontogeny. Their levels varied according to age, tissue, and mouse strain analyzed. This differential expression may contribute to the distinct patterns of mouse susceptibility/resistance to infectious and noninfectious disorders.
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spelling pubmed-40092012014-05-14 Differential Transcript Profiles of MHC Class Ib(Qa-1, Qa-2, and Qa-10) and Aire Genes during the Ontogeny of Thymus and Other Tissues Melo-Lima, Breno Luiz Evangelista, Adriane Feijó de Magalhães, Danielle Aparecida Rosa Passos, Geraldo Aleixo Moreau, Philippe Donadi, Eduardo Antonio J Immunol Res Research Article Qa-2 and Qa-1 are murine nonclassical MHC class I molecules involved in the modulation of immune responses by interacting with T CD8(+) and NK cell inhibitory receptors. During thymic education, the Aire gene imposes the expression of thousands of tissue-related antigens in the thymic medulla, permitting the negative selection events. Aiming to characterize the transcriptional profiles of nonclassical MHC class I genes in spatial-temporal association with the Aire expression, we evaluated the gene expression of H2-Q7(Qa-2), H2-T23(Qa-1), H2-Q10(Qa-10), and Aire during fetal and postnatal development of thymus and other tissues. In the thymus, H2-Q7(Qa-2) transcripts were detected at high levels throughout development and were positively correlated with Aire expression during fetal ages. H2-Q7(Qa-2) and H2-T23(Qa-1) showed distinct expression patterns with gradual increasing levels according to age in most tissues analyzed. H2-Q10(Qa-10) was preferentially expressed by the liver. The Aire transcriptional profile showed increased levels during the fetal period and was detectable in postnatal ages in the thymus. Overall, nonclassical MHC class I genes started to be expressed early during the ontogeny. Their levels varied according to age, tissue, and mouse strain analyzed. This differential expression may contribute to the distinct patterns of mouse susceptibility/resistance to infectious and noninfectious disorders. Hindawi Publishing Corporation 2014 2014-04-16 /pmc/articles/PMC4009201/ /pubmed/24829926 http://dx.doi.org/10.1155/2014/159247 Text en Copyright © 2014 Breno Luiz Melo-Lima et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Melo-Lima, Breno Luiz
Evangelista, Adriane Feijó
de Magalhães, Danielle Aparecida Rosa
Passos, Geraldo Aleixo
Moreau, Philippe
Donadi, Eduardo Antonio
Differential Transcript Profiles of MHC Class Ib(Qa-1, Qa-2, and Qa-10) and Aire Genes during the Ontogeny of Thymus and Other Tissues
title Differential Transcript Profiles of MHC Class Ib(Qa-1, Qa-2, and Qa-10) and Aire Genes during the Ontogeny of Thymus and Other Tissues
title_full Differential Transcript Profiles of MHC Class Ib(Qa-1, Qa-2, and Qa-10) and Aire Genes during the Ontogeny of Thymus and Other Tissues
title_fullStr Differential Transcript Profiles of MHC Class Ib(Qa-1, Qa-2, and Qa-10) and Aire Genes during the Ontogeny of Thymus and Other Tissues
title_full_unstemmed Differential Transcript Profiles of MHC Class Ib(Qa-1, Qa-2, and Qa-10) and Aire Genes during the Ontogeny of Thymus and Other Tissues
title_short Differential Transcript Profiles of MHC Class Ib(Qa-1, Qa-2, and Qa-10) and Aire Genes during the Ontogeny of Thymus and Other Tissues
title_sort differential transcript profiles of mhc class ib(qa-1, qa-2, and qa-10) and aire genes during the ontogeny of thymus and other tissues
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009201/
https://www.ncbi.nlm.nih.gov/pubmed/24829926
http://dx.doi.org/10.1155/2014/159247
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