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Small Islets Transplantation Superiority to Large Ones: Implications from Islet Microcirculation and Revascularization

Pancreatic islet transplantation is a promising therapy to regain glycemic control in diabetic patients. The selection of ideal grafts is the basis to guarantee short-term effectivity and longevity of the transplanted islets. Contradictory to the traditional notion, recent findings implied the super...

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Autores principales: Li, Wenjuan, Zhao, Ruxing, Liu, Jidong, Tian, Meng, Lu, Yiran, He, Tianyi, Cheng, Meng, Liang, Kai, Li, Xia, Wang, Xiangdong, Sun, Yu, Chen, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009214/
https://www.ncbi.nlm.nih.gov/pubmed/24829922
http://dx.doi.org/10.1155/2014/192093
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author Li, Wenjuan
Zhao, Ruxing
Liu, Jidong
Tian, Meng
Lu, Yiran
He, Tianyi
Cheng, Meng
Liang, Kai
Li, Xia
Wang, Xiangdong
Sun, Yu
Chen, Li
author_facet Li, Wenjuan
Zhao, Ruxing
Liu, Jidong
Tian, Meng
Lu, Yiran
He, Tianyi
Cheng, Meng
Liang, Kai
Li, Xia
Wang, Xiangdong
Sun, Yu
Chen, Li
author_sort Li, Wenjuan
collection PubMed
description Pancreatic islet transplantation is a promising therapy to regain glycemic control in diabetic patients. The selection of ideal grafts is the basis to guarantee short-term effectivity and longevity of the transplanted islets. Contradictory to the traditional notion, recent findings implied the superiority of small islets for better transplantation outcomes rather than the large and intact ones. However, the mechanisms remain to be elucidated. Recent evidences emphasized the major impact of microcirculation on islet β-cell mass and function. And potentials in islet graft revascularization are crucial for their survival and preserved function in the recipient. In this study, we verified the distinct histological phenotype and functionality of small islets versus large ones both in vitro and in vivo. With efforts to exploring the differences in microcirculation and revascularization of islet grafts, we further evaluated local expressions of angiotensin and vascular endothelial growth factor A (VEGF-A) at different levels. Our findings reveal that, apart from the higher density of insulin-producing β-cells, small islets express less angiotensin and more angiotrophic VEGF-A. We therefore hypothesized a logical explanation of the small islet superiority for transplantation outcome from the aspects of facilitated microcirculation and revascularization intrinsically in small islets.
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spelling pubmed-40092142014-05-14 Small Islets Transplantation Superiority to Large Ones: Implications from Islet Microcirculation and Revascularization Li, Wenjuan Zhao, Ruxing Liu, Jidong Tian, Meng Lu, Yiran He, Tianyi Cheng, Meng Liang, Kai Li, Xia Wang, Xiangdong Sun, Yu Chen, Li J Diabetes Res Research Article Pancreatic islet transplantation is a promising therapy to regain glycemic control in diabetic patients. The selection of ideal grafts is the basis to guarantee short-term effectivity and longevity of the transplanted islets. Contradictory to the traditional notion, recent findings implied the superiority of small islets for better transplantation outcomes rather than the large and intact ones. However, the mechanisms remain to be elucidated. Recent evidences emphasized the major impact of microcirculation on islet β-cell mass and function. And potentials in islet graft revascularization are crucial for their survival and preserved function in the recipient. In this study, we verified the distinct histological phenotype and functionality of small islets versus large ones both in vitro and in vivo. With efforts to exploring the differences in microcirculation and revascularization of islet grafts, we further evaluated local expressions of angiotensin and vascular endothelial growth factor A (VEGF-A) at different levels. Our findings reveal that, apart from the higher density of insulin-producing β-cells, small islets express less angiotensin and more angiotrophic VEGF-A. We therefore hypothesized a logical explanation of the small islet superiority for transplantation outcome from the aspects of facilitated microcirculation and revascularization intrinsically in small islets. Hindawi Publishing Corporation 2014 2014-04-16 /pmc/articles/PMC4009214/ /pubmed/24829922 http://dx.doi.org/10.1155/2014/192093 Text en Copyright © 2014 Wenjuan Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Wenjuan
Zhao, Ruxing
Liu, Jidong
Tian, Meng
Lu, Yiran
He, Tianyi
Cheng, Meng
Liang, Kai
Li, Xia
Wang, Xiangdong
Sun, Yu
Chen, Li
Small Islets Transplantation Superiority to Large Ones: Implications from Islet Microcirculation and Revascularization
title Small Islets Transplantation Superiority to Large Ones: Implications from Islet Microcirculation and Revascularization
title_full Small Islets Transplantation Superiority to Large Ones: Implications from Islet Microcirculation and Revascularization
title_fullStr Small Islets Transplantation Superiority to Large Ones: Implications from Islet Microcirculation and Revascularization
title_full_unstemmed Small Islets Transplantation Superiority to Large Ones: Implications from Islet Microcirculation and Revascularization
title_short Small Islets Transplantation Superiority to Large Ones: Implications from Islet Microcirculation and Revascularization
title_sort small islets transplantation superiority to large ones: implications from islet microcirculation and revascularization
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009214/
https://www.ncbi.nlm.nih.gov/pubmed/24829922
http://dx.doi.org/10.1155/2014/192093
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