A Two-Step Lyssavirus Real-Time Polymerase Chain Reaction Using Degenerate Primers with Superior Sensitivity to the Fluorescent Antigen Test

A generic two-step lyssavirus real-time reverse transcriptase polymerase chain reaction (qRT-PCR), based on a nested PCR strategy, was validated for the detection of different lyssavirus species. Primers with 17 to 30% of degenerate bases were used in both consecutive steps. The assay could accurate...

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Detalles Bibliográficos
Autores principales: Suin, Vanessa, Nazé, Florence, Francart, Aurélie, Lamoral, Sophie, De Craeye, Stéphane, Kalai, Michael, Van Gucht, Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009295/
https://www.ncbi.nlm.nih.gov/pubmed/24822188
http://dx.doi.org/10.1155/2014/256175

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