Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC

A reversed-phase high-performance liquid chromatographic method has been developed to quantitatively determine matrine in liquid crystal nanoparticles. The chromatographic method is carried out using an isocratic system. The mobile phase was composed of methanol-PBS(pH6.8)-triethylamine (50 : 50 : 0...

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Autores principales: Peng, Xinsheng, Li, Baohong, Hu, Min, Ling, Yahao, Tian, Yuan, Zhou, Yanxing, Zhou, Yanfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009329/
https://www.ncbi.nlm.nih.gov/pubmed/24834359
http://dx.doi.org/10.1155/2014/368682
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author Peng, Xinsheng
Li, Baohong
Hu, Min
Ling, Yahao
Tian, Yuan
Zhou, Yanxing
Zhou, Yanfang
author_facet Peng, Xinsheng
Li, Baohong
Hu, Min
Ling, Yahao
Tian, Yuan
Zhou, Yanxing
Zhou, Yanfang
author_sort Peng, Xinsheng
collection PubMed
description A reversed-phase high-performance liquid chromatographic method has been developed to quantitatively determine matrine in liquid crystal nanoparticles. The chromatographic method is carried out using an isocratic system. The mobile phase was composed of methanol-PBS(pH6.8)-triethylamine (50 : 50 : 0.1%) with a flow rate of 1 mL/min with SPD-20A UV/vis detector and the detection wavelength was at 220 nm. The linearity of matrine is in the range of 1.6 to 200.0 μg/mL. The regression equation is y = 10706x − 2959 (R (2) = 1.0). The average recovery is 101.7%; RSD = 2.22%  (n = 9). This method provides a simple and accurate strategy to determine matrine in liquid crystalline nanoparticle.
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spelling pubmed-40093292014-05-15 Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC Peng, Xinsheng Li, Baohong Hu, Min Ling, Yahao Tian, Yuan Zhou, Yanxing Zhou, Yanfang J Anal Methods Chem Research Article A reversed-phase high-performance liquid chromatographic method has been developed to quantitatively determine matrine in liquid crystal nanoparticles. The chromatographic method is carried out using an isocratic system. The mobile phase was composed of methanol-PBS(pH6.8)-triethylamine (50 : 50 : 0.1%) with a flow rate of 1 mL/min with SPD-20A UV/vis detector and the detection wavelength was at 220 nm. The linearity of matrine is in the range of 1.6 to 200.0 μg/mL. The regression equation is y = 10706x − 2959 (R (2) = 1.0). The average recovery is 101.7%; RSD = 2.22%  (n = 9). This method provides a simple and accurate strategy to determine matrine in liquid crystalline nanoparticle. Hindawi Publishing Corporation 2014 2014-04-14 /pmc/articles/PMC4009329/ /pubmed/24834359 http://dx.doi.org/10.1155/2014/368682 Text en Copyright © 2014 Xinsheng Peng et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Peng, Xinsheng
Li, Baohong
Hu, Min
Ling, Yahao
Tian, Yuan
Zhou, Yanxing
Zhou, Yanfang
Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC
title Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC
title_full Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC
title_fullStr Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC
title_full_unstemmed Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC
title_short Quantitative Analysis of Matrine in Liquid Crystalline Nanoparticles by HPLC
title_sort quantitative analysis of matrine in liquid crystalline nanoparticles by hplc
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009329/
https://www.ncbi.nlm.nih.gov/pubmed/24834359
http://dx.doi.org/10.1155/2014/368682
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