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High-resolution mapping defines the cooperative architecture of Polycomb response elements

Polycomb-mediated chromatin repression modulates gene expression during development in metazoans. Binding of multiple sequence-specific factors at discrete Polycomb response elements (PREs) is thought to recruit repressive complexes that spread across an extended chromatin domain. To dissect the str...

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Detalles Bibliográficos
Autores principales: Orsi, Guillermo A., Kasinathan, Sivakanthan, Hughes, Kelly T., Saminadin-Peter, Sarah, Henikoff, Steven, Ahmad, Kami
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009610/
https://www.ncbi.nlm.nih.gov/pubmed/24668908
http://dx.doi.org/10.1101/gr.163642.113
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author Orsi, Guillermo A.
Kasinathan, Sivakanthan
Hughes, Kelly T.
Saminadin-Peter, Sarah
Henikoff, Steven
Ahmad, Kami
author_facet Orsi, Guillermo A.
Kasinathan, Sivakanthan
Hughes, Kelly T.
Saminadin-Peter, Sarah
Henikoff, Steven
Ahmad, Kami
author_sort Orsi, Guillermo A.
collection PubMed
description Polycomb-mediated chromatin repression modulates gene expression during development in metazoans. Binding of multiple sequence-specific factors at discrete Polycomb response elements (PREs) is thought to recruit repressive complexes that spread across an extended chromatin domain. To dissect the structure of PREs, we applied high-resolution mapping of nonhistone chromatin proteins in native chromatin of Drosophila cells. Analysis of occupied sites reveal interactions between transcription factors that stabilize Polycomb anchoring to DNA, and implicate the general transcription factor ADF1 as a novel PRE component. By comparing two Drosophila cell lines with differential chromatin states, we provide evidence that repression is accomplished by enhanced Polycomb recruitment both to PREs and to target promoters of repressed genes. These results suggest that the stability of multifactor complexes at promoters and regulatory elements is a crucial aspect of developmentally regulated gene expression.
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spelling pubmed-40096102014-11-01 High-resolution mapping defines the cooperative architecture of Polycomb response elements Orsi, Guillermo A. Kasinathan, Sivakanthan Hughes, Kelly T. Saminadin-Peter, Sarah Henikoff, Steven Ahmad, Kami Genome Res Research Polycomb-mediated chromatin repression modulates gene expression during development in metazoans. Binding of multiple sequence-specific factors at discrete Polycomb response elements (PREs) is thought to recruit repressive complexes that spread across an extended chromatin domain. To dissect the structure of PREs, we applied high-resolution mapping of nonhistone chromatin proteins in native chromatin of Drosophila cells. Analysis of occupied sites reveal interactions between transcription factors that stabilize Polycomb anchoring to DNA, and implicate the general transcription factor ADF1 as a novel PRE component. By comparing two Drosophila cell lines with differential chromatin states, we provide evidence that repression is accomplished by enhanced Polycomb recruitment both to PREs and to target promoters of repressed genes. These results suggest that the stability of multifactor complexes at promoters and regulatory elements is a crucial aspect of developmentally regulated gene expression. Cold Spring Harbor Laboratory Press 2014-05 /pmc/articles/PMC4009610/ /pubmed/24668908 http://dx.doi.org/10.1101/gr.163642.113 Text en © 2014 Orsi et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research
Orsi, Guillermo A.
Kasinathan, Sivakanthan
Hughes, Kelly T.
Saminadin-Peter, Sarah
Henikoff, Steven
Ahmad, Kami
High-resolution mapping defines the cooperative architecture of Polycomb response elements
title High-resolution mapping defines the cooperative architecture of Polycomb response elements
title_full High-resolution mapping defines the cooperative architecture of Polycomb response elements
title_fullStr High-resolution mapping defines the cooperative architecture of Polycomb response elements
title_full_unstemmed High-resolution mapping defines the cooperative architecture of Polycomb response elements
title_short High-resolution mapping defines the cooperative architecture of Polycomb response elements
title_sort high-resolution mapping defines the cooperative architecture of polycomb response elements
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4009610/
https://www.ncbi.nlm.nih.gov/pubmed/24668908
http://dx.doi.org/10.1101/gr.163642.113
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