Cargando…

BioPen: direct writing of functional materials at the point of care

Rapid and precise patterning of functional biomaterials is desirable for point-of-care (POC) tissue engineering and diagnostics. However, existing technologies such as dip-pen nanolithography and inkjet printing are currently unsuitable for POC applications due to issues of cost and portability. Her...

Descripción completa

Detalles Bibliográficos
Autores principales: Han, Yu Long, Hu, Jie, Genin, Guy M., Lu, Tian Jian, Xu, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4010928/
https://www.ncbi.nlm.nih.gov/pubmed/24799039
http://dx.doi.org/10.1038/srep04872
_version_ 1782479929347145728
author Han, Yu Long
Hu, Jie
Genin, Guy M.
Lu, Tian Jian
Xu, Feng
author_facet Han, Yu Long
Hu, Jie
Genin, Guy M.
Lu, Tian Jian
Xu, Feng
author_sort Han, Yu Long
collection PubMed
description Rapid and precise patterning of functional biomaterials is desirable for point-of-care (POC) tissue engineering and diagnostics. However, existing technologies such as dip-pen nanolithography and inkjet printing are currently unsuitable for POC applications due to issues of cost and portability. Here, we report the development of ‘BioPen', a portable tool for continuous, defined and scalable deposition of functional materials with micrometer spatial resolution and nanolitre volumetric resolution. BioPen is based upon the ballpoint pen but with multiple “ink sources” (functional material solutions) and with an apparatus that can be optimized for writing living cells, proteins, nucleic acids, etc. We demonstrate POC detection of human immunodeficiency virus type 1 (HIV-1) nucleic acid by writing on paper with BioPen using “ink” consisting of nucleic acid probes and nucleic acid-modified gold nanoparticles. We also demonstrate POC tissue engineering by writing a continuous pattern of living, functional, interconnected cells with a defined extracellular environment. Because it is simple, accurate, inexpensive and portable, BioPen has broad potential for POC detection of diagnostic biomarkers, and for POC engineering of tissues for a range of healing applications.
format Online
Article
Text
id pubmed-4010928
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-40109282014-05-06 BioPen: direct writing of functional materials at the point of care Han, Yu Long Hu, Jie Genin, Guy M. Lu, Tian Jian Xu, Feng Sci Rep Article Rapid and precise patterning of functional biomaterials is desirable for point-of-care (POC) tissue engineering and diagnostics. However, existing technologies such as dip-pen nanolithography and inkjet printing are currently unsuitable for POC applications due to issues of cost and portability. Here, we report the development of ‘BioPen', a portable tool for continuous, defined and scalable deposition of functional materials with micrometer spatial resolution and nanolitre volumetric resolution. BioPen is based upon the ballpoint pen but with multiple “ink sources” (functional material solutions) and with an apparatus that can be optimized for writing living cells, proteins, nucleic acids, etc. We demonstrate POC detection of human immunodeficiency virus type 1 (HIV-1) nucleic acid by writing on paper with BioPen using “ink” consisting of nucleic acid probes and nucleic acid-modified gold nanoparticles. We also demonstrate POC tissue engineering by writing a continuous pattern of living, functional, interconnected cells with a defined extracellular environment. Because it is simple, accurate, inexpensive and portable, BioPen has broad potential for POC detection of diagnostic biomarkers, and for POC engineering of tissues for a range of healing applications. Nature Publishing Group 2014-05-06 /pmc/articles/PMC4010928/ /pubmed/24799039 http://dx.doi.org/10.1038/srep04872 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Han, Yu Long
Hu, Jie
Genin, Guy M.
Lu, Tian Jian
Xu, Feng
BioPen: direct writing of functional materials at the point of care
title BioPen: direct writing of functional materials at the point of care
title_full BioPen: direct writing of functional materials at the point of care
title_fullStr BioPen: direct writing of functional materials at the point of care
title_full_unstemmed BioPen: direct writing of functional materials at the point of care
title_short BioPen: direct writing of functional materials at the point of care
title_sort biopen: direct writing of functional materials at the point of care
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4010928/
https://www.ncbi.nlm.nih.gov/pubmed/24799039
http://dx.doi.org/10.1038/srep04872
work_keys_str_mv AT hanyulong biopendirectwritingoffunctionalmaterialsatthepointofcare
AT hujie biopendirectwritingoffunctionalmaterialsatthepointofcare
AT geninguym biopendirectwritingoffunctionalmaterialsatthepointofcare
AT lutianjian biopendirectwritingoffunctionalmaterialsatthepointofcare
AT xufeng biopendirectwritingoffunctionalmaterialsatthepointofcare