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BioPen: direct writing of functional materials at the point of care
Rapid and precise patterning of functional biomaterials is desirable for point-of-care (POC) tissue engineering and diagnostics. However, existing technologies such as dip-pen nanolithography and inkjet printing are currently unsuitable for POC applications due to issues of cost and portability. Her...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4010928/ https://www.ncbi.nlm.nih.gov/pubmed/24799039 http://dx.doi.org/10.1038/srep04872 |
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author | Han, Yu Long Hu, Jie Genin, Guy M. Lu, Tian Jian Xu, Feng |
author_facet | Han, Yu Long Hu, Jie Genin, Guy M. Lu, Tian Jian Xu, Feng |
author_sort | Han, Yu Long |
collection | PubMed |
description | Rapid and precise patterning of functional biomaterials is desirable for point-of-care (POC) tissue engineering and diagnostics. However, existing technologies such as dip-pen nanolithography and inkjet printing are currently unsuitable for POC applications due to issues of cost and portability. Here, we report the development of ‘BioPen', a portable tool for continuous, defined and scalable deposition of functional materials with micrometer spatial resolution and nanolitre volumetric resolution. BioPen is based upon the ballpoint pen but with multiple “ink sources” (functional material solutions) and with an apparatus that can be optimized for writing living cells, proteins, nucleic acids, etc. We demonstrate POC detection of human immunodeficiency virus type 1 (HIV-1) nucleic acid by writing on paper with BioPen using “ink” consisting of nucleic acid probes and nucleic acid-modified gold nanoparticles. We also demonstrate POC tissue engineering by writing a continuous pattern of living, functional, interconnected cells with a defined extracellular environment. Because it is simple, accurate, inexpensive and portable, BioPen has broad potential for POC detection of diagnostic biomarkers, and for POC engineering of tissues for a range of healing applications. |
format | Online Article Text |
id | pubmed-4010928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-40109282014-05-06 BioPen: direct writing of functional materials at the point of care Han, Yu Long Hu, Jie Genin, Guy M. Lu, Tian Jian Xu, Feng Sci Rep Article Rapid and precise patterning of functional biomaterials is desirable for point-of-care (POC) tissue engineering and diagnostics. However, existing technologies such as dip-pen nanolithography and inkjet printing are currently unsuitable for POC applications due to issues of cost and portability. Here, we report the development of ‘BioPen', a portable tool for continuous, defined and scalable deposition of functional materials with micrometer spatial resolution and nanolitre volumetric resolution. BioPen is based upon the ballpoint pen but with multiple “ink sources” (functional material solutions) and with an apparatus that can be optimized for writing living cells, proteins, nucleic acids, etc. We demonstrate POC detection of human immunodeficiency virus type 1 (HIV-1) nucleic acid by writing on paper with BioPen using “ink” consisting of nucleic acid probes and nucleic acid-modified gold nanoparticles. We also demonstrate POC tissue engineering by writing a continuous pattern of living, functional, interconnected cells with a defined extracellular environment. Because it is simple, accurate, inexpensive and portable, BioPen has broad potential for POC detection of diagnostic biomarkers, and for POC engineering of tissues for a range of healing applications. Nature Publishing Group 2014-05-06 /pmc/articles/PMC4010928/ /pubmed/24799039 http://dx.doi.org/10.1038/srep04872 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Han, Yu Long Hu, Jie Genin, Guy M. Lu, Tian Jian Xu, Feng BioPen: direct writing of functional materials at the point of care |
title | BioPen: direct writing of functional materials at the point of care |
title_full | BioPen: direct writing of functional materials at the point of care |
title_fullStr | BioPen: direct writing of functional materials at the point of care |
title_full_unstemmed | BioPen: direct writing of functional materials at the point of care |
title_short | BioPen: direct writing of functional materials at the point of care |
title_sort | biopen: direct writing of functional materials at the point of care |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4010928/ https://www.ncbi.nlm.nih.gov/pubmed/24799039 http://dx.doi.org/10.1038/srep04872 |
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