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Variants of a Thermus aquaticus DNA Polymerase with Increased Selectivity for Applications in Allele- and Methylation-Specific Amplification

The selectivity of DNA polymerases is crucial for many applications. For example, high discrimination between the extension of matched versus mismatched primer termini is desired for the detection of a single nucleotide variation at a particular locus within the genome. Here we describe the generati...

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Detalles Bibliográficos
Autores principales:	Drum, Matthias, Kranaster, Ramon, Ewald, Christina, Blasczyk, Rainer, Marx, Andreas
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4011760/ https://www.ncbi.nlm.nih.gov/pubmed/24800860 http://dx.doi.org/10.1371/journal.pone.0096640

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