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Down-Regulation of Sox11 Is Required for Efficient Osteogenic Differentiation of Adipose-Derived Stem Cells

Adipose-derived stem cells represent a type of mesenchymal stem cells with the attendant capacity to self-renew and differentiate into multiple cell lineages. We have performed a microarray-based gene expression profiling of osteogenic differentiation and found that the transcription factor Sox11 is...

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Autores principales: Choi, Mi Kyung, Seong, Ikjoo, Kang, Seon Ah, Kim, Jaesang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012083/
https://www.ncbi.nlm.nih.gov/pubmed/24722414
http://dx.doi.org/10.14348/molcells.2014.0021
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author Choi, Mi Kyung
Seong, Ikjoo
Kang, Seon Ah
Kim, Jaesang
author_facet Choi, Mi Kyung
Seong, Ikjoo
Kang, Seon Ah
Kim, Jaesang
author_sort Choi, Mi Kyung
collection PubMed
description Adipose-derived stem cells represent a type of mesenchymal stem cells with the attendant capacity to self-renew and differentiate into multiple cell lineages. We have performed a microarray-based gene expression profiling of osteogenic differentiation and found that the transcription factor Sox11 is down-regulated during the process. Functional assays demonstrate that down-regulation of Sox11 is required for an efficient differentiation. Furthermore, results from forced expression of constitutively-active and dominant-negative derivatives of Sox11 indicate that Sox11 functions as a transcriptional activator in inhibiting osteogenesis. Sox11 thus represents a novel regulator of osteogenesis whose expression and activity can be potentially manipulated for controlled differentiation.
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spelling pubmed-40120832014-05-09 Down-Regulation of Sox11 Is Required for Efficient Osteogenic Differentiation of Adipose-Derived Stem Cells Choi, Mi Kyung Seong, Ikjoo Kang, Seon Ah Kim, Jaesang Mol Cells Research Article Adipose-derived stem cells represent a type of mesenchymal stem cells with the attendant capacity to self-renew and differentiate into multiple cell lineages. We have performed a microarray-based gene expression profiling of osteogenic differentiation and found that the transcription factor Sox11 is down-regulated during the process. Functional assays demonstrate that down-regulation of Sox11 is required for an efficient differentiation. Furthermore, results from forced expression of constitutively-active and dominant-negative derivatives of Sox11 indicate that Sox11 functions as a transcriptional activator in inhibiting osteogenesis. Sox11 thus represents a novel regulator of osteogenesis whose expression and activity can be potentially manipulated for controlled differentiation. Korean Society for Molecular and Cellular Biology 2014-04-30 2014-04-07 /pmc/articles/PMC4012083/ /pubmed/24722414 http://dx.doi.org/10.14348/molcells.2014.0021 Text en The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/.
spellingShingle Research Article
Choi, Mi Kyung
Seong, Ikjoo
Kang, Seon Ah
Kim, Jaesang
Down-Regulation of Sox11 Is Required for Efficient Osteogenic Differentiation of Adipose-Derived Stem Cells
title Down-Regulation of Sox11 Is Required for Efficient Osteogenic Differentiation of Adipose-Derived Stem Cells
title_full Down-Regulation of Sox11 Is Required for Efficient Osteogenic Differentiation of Adipose-Derived Stem Cells
title_fullStr Down-Regulation of Sox11 Is Required for Efficient Osteogenic Differentiation of Adipose-Derived Stem Cells
title_full_unstemmed Down-Regulation of Sox11 Is Required for Efficient Osteogenic Differentiation of Adipose-Derived Stem Cells
title_short Down-Regulation of Sox11 Is Required for Efficient Osteogenic Differentiation of Adipose-Derived Stem Cells
title_sort down-regulation of sox11 is required for efficient osteogenic differentiation of adipose-derived stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012083/
https://www.ncbi.nlm.nih.gov/pubmed/24722414
http://dx.doi.org/10.14348/molcells.2014.0021
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