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Estimation of lactoferrin levels in gingival crevicular fluid before and after periodontal therapy in patients with chronic periodontitis

BACKGROUND: The lactoferrin (LF) is an iron binding protein present specifically and in abundance in the secondary granules of polymorphonuclear leukocytes (PMN's). It has been suggested that LF in crevicular fluid is a useful marker of PMN activity. Hence, this study aimed to estimate the leve...

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Autores principales: Kivadasannavar, Anasuya V., Pudakalakatti, Pushpa S., Hattarki, Sanjeevini A., Kambali, Soumya S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012112/
https://www.ncbi.nlm.nih.gov/pubmed/24808691
http://dx.doi.org/10.4103/0976-237X.128657
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author Kivadasannavar, Anasuya V.
Pudakalakatti, Pushpa S.
Hattarki, Sanjeevini A.
Kambali, Soumya S.
author_facet Kivadasannavar, Anasuya V.
Pudakalakatti, Pushpa S.
Hattarki, Sanjeevini A.
Kambali, Soumya S.
author_sort Kivadasannavar, Anasuya V.
collection PubMed
description BACKGROUND: The lactoferrin (LF) is an iron binding protein present specifically and in abundance in the secondary granules of polymorphonuclear leukocytes (PMN's). It has been suggested that LF in crevicular fluid is a useful marker of PMN activity. Hence, this study aimed to estimate the levels of LF in gingival crevicular fluid (GCF) before and after surgical therapy in chronic periodontitis patients to assess the validity of LF in monitoring of treatment results. MATERIALS AND METHODS: A total of 30 patients with chronic periodontitis having probing depth of ≥5 mm who were scheduled for periodontal surgery were included in the study. The clinical parameters were recorded and GCF samples were obtained 2 weeks after scaling and root planing and 2 weeks after conventional flap technique. The samples collected were then assayed for LF using Enzyme-linked immunosorbent assay (ELISA). RESULTS: The results showed that LF levels decreased significantly from 266.53 ± 75.86 to 195.47 ± 74.53 after scaling and root planing. There was further significant reduction in LF levels to 90.42 ± 32.89 following 2 weeks of periodontal surgery, indicating decrease in inflammation. CONCLUSION: There is a significant reduction in GCF LF levels following periodontal surgery. Hence, LF levels in GCF could serve as a useful marker for monitoring of periodontal treatment results.
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spelling pubmed-40121122014-05-07 Estimation of lactoferrin levels in gingival crevicular fluid before and after periodontal therapy in patients with chronic periodontitis Kivadasannavar, Anasuya V. Pudakalakatti, Pushpa S. Hattarki, Sanjeevini A. Kambali, Soumya S. Contemp Clin Dent Original Article BACKGROUND: The lactoferrin (LF) is an iron binding protein present specifically and in abundance in the secondary granules of polymorphonuclear leukocytes (PMN's). It has been suggested that LF in crevicular fluid is a useful marker of PMN activity. Hence, this study aimed to estimate the levels of LF in gingival crevicular fluid (GCF) before and after surgical therapy in chronic periodontitis patients to assess the validity of LF in monitoring of treatment results. MATERIALS AND METHODS: A total of 30 patients with chronic periodontitis having probing depth of ≥5 mm who were scheduled for periodontal surgery were included in the study. The clinical parameters were recorded and GCF samples were obtained 2 weeks after scaling and root planing and 2 weeks after conventional flap technique. The samples collected were then assayed for LF using Enzyme-linked immunosorbent assay (ELISA). RESULTS: The results showed that LF levels decreased significantly from 266.53 ± 75.86 to 195.47 ± 74.53 after scaling and root planing. There was further significant reduction in LF levels to 90.42 ± 32.89 following 2 weeks of periodontal surgery, indicating decrease in inflammation. CONCLUSION: There is a significant reduction in GCF LF levels following periodontal surgery. Hence, LF levels in GCF could serve as a useful marker for monitoring of periodontal treatment results. Medknow Publications & Media Pvt Ltd 2014 /pmc/articles/PMC4012112/ /pubmed/24808691 http://dx.doi.org/10.4103/0976-237X.128657 Text en Copyright: © Contemporary Clinical Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kivadasannavar, Anasuya V.
Pudakalakatti, Pushpa S.
Hattarki, Sanjeevini A.
Kambali, Soumya S.
Estimation of lactoferrin levels in gingival crevicular fluid before and after periodontal therapy in patients with chronic periodontitis
title Estimation of lactoferrin levels in gingival crevicular fluid before and after periodontal therapy in patients with chronic periodontitis
title_full Estimation of lactoferrin levels in gingival crevicular fluid before and after periodontal therapy in patients with chronic periodontitis
title_fullStr Estimation of lactoferrin levels in gingival crevicular fluid before and after periodontal therapy in patients with chronic periodontitis
title_full_unstemmed Estimation of lactoferrin levels in gingival crevicular fluid before and after periodontal therapy in patients with chronic periodontitis
title_short Estimation of lactoferrin levels in gingival crevicular fluid before and after periodontal therapy in patients with chronic periodontitis
title_sort estimation of lactoferrin levels in gingival crevicular fluid before and after periodontal therapy in patients with chronic periodontitis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012112/
https://www.ncbi.nlm.nih.gov/pubmed/24808691
http://dx.doi.org/10.4103/0976-237X.128657
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