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Protective effect of Cissus quadrangularis Linn. on diabetes induced delayed fetal skeletal ossification

BACKGROUND: Delayed fetal skeletal ossification is one of the known complications of maternal diabetes. OBJECTIVE: The present study was designed to evaluate the protective role of petroleum ether extract of Cissus quadrangularis (PECQ) on diabetes-induced delayed fetal skeletal ossification. MATERI...

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Autores principales: Sirasanagandla, Srinivasa Rao, Ranganath Pai, K. Sreedhara, Potu, Bhagath Kumar, Bhat, Kumar MR
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012358/
https://www.ncbi.nlm.nih.gov/pubmed/24812472
http://dx.doi.org/10.4103/0975-9476.128852
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author Sirasanagandla, Srinivasa Rao
Ranganath Pai, K. Sreedhara
Potu, Bhagath Kumar
Bhat, Kumar MR
author_facet Sirasanagandla, Srinivasa Rao
Ranganath Pai, K. Sreedhara
Potu, Bhagath Kumar
Bhat, Kumar MR
author_sort Sirasanagandla, Srinivasa Rao
collection PubMed
description BACKGROUND: Delayed fetal skeletal ossification is one of the known complications of maternal diabetes. OBJECTIVE: The present study was designed to evaluate the protective role of petroleum ether extract of Cissus quadrangularis (PECQ) on diabetes-induced delayed fetal skeletal ossification. MATERIALS AND METHODS: Female Wistar rats were rendered diabetic with streptozotocin (STZ, 40 mg/kg, intraperitonial) before mating. After confirmation of pregnancy, the pregnant rats were divided into three groups: normal control group, diabetic control group, and diabetic + CQ group. The diabetic + CQ group pregnant rats were treated with PECQ (500 mg/kg body weight) throughout their gestation period. Immediately after delivery, pups were collected from all three groups and processed for alizarin red S–alcian blue staining in order to examine the pattern of skeletal ossification. RESULTS: Fewer ossification centers and decreased extent of ossification of forelimb and hindlimb bones were observed in the neonatal pups of diabetic control group as compared to those in the normal control group. PECQ pretreatment significantly restored the ossification centers and improved the extent of ossification of forelimb and hindlimb bones in the neonatal pups of diabetic + CQ group as compared to those in the diabetic control group. CONCLUSIONS: The results suggested that PECQ treatment is effective against diabetes-induced delayed fetal skeletal ossification. However, further studies on the isolation and characterization of active constituents of PECQ, which can cross the placental barrier and are responsible for the bone anabolic activity are warranted.
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spelling pubmed-40123582014-05-08 Protective effect of Cissus quadrangularis Linn. on diabetes induced delayed fetal skeletal ossification Sirasanagandla, Srinivasa Rao Ranganath Pai, K. Sreedhara Potu, Bhagath Kumar Bhat, Kumar MR J Ayurveda Integr Med Original Research Article BACKGROUND: Delayed fetal skeletal ossification is one of the known complications of maternal diabetes. OBJECTIVE: The present study was designed to evaluate the protective role of petroleum ether extract of Cissus quadrangularis (PECQ) on diabetes-induced delayed fetal skeletal ossification. MATERIALS AND METHODS: Female Wistar rats were rendered diabetic with streptozotocin (STZ, 40 mg/kg, intraperitonial) before mating. After confirmation of pregnancy, the pregnant rats were divided into three groups: normal control group, diabetic control group, and diabetic + CQ group. The diabetic + CQ group pregnant rats were treated with PECQ (500 mg/kg body weight) throughout their gestation period. Immediately after delivery, pups were collected from all three groups and processed for alizarin red S–alcian blue staining in order to examine the pattern of skeletal ossification. RESULTS: Fewer ossification centers and decreased extent of ossification of forelimb and hindlimb bones were observed in the neonatal pups of diabetic control group as compared to those in the normal control group. PECQ pretreatment significantly restored the ossification centers and improved the extent of ossification of forelimb and hindlimb bones in the neonatal pups of diabetic + CQ group as compared to those in the diabetic control group. CONCLUSIONS: The results suggested that PECQ treatment is effective against diabetes-induced delayed fetal skeletal ossification. However, further studies on the isolation and characterization of active constituents of PECQ, which can cross the placental barrier and are responsible for the bone anabolic activity are warranted. Medknow Publications & Media Pvt Ltd 2014 /pmc/articles/PMC4012358/ /pubmed/24812472 http://dx.doi.org/10.4103/0975-9476.128852 Text en Copyright: © Journal of Ayurveda and Integrative Medicine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Article
Sirasanagandla, Srinivasa Rao
Ranganath Pai, K. Sreedhara
Potu, Bhagath Kumar
Bhat, Kumar MR
Protective effect of Cissus quadrangularis Linn. on diabetes induced delayed fetal skeletal ossification
title Protective effect of Cissus quadrangularis Linn. on diabetes induced delayed fetal skeletal ossification
title_full Protective effect of Cissus quadrangularis Linn. on diabetes induced delayed fetal skeletal ossification
title_fullStr Protective effect of Cissus quadrangularis Linn. on diabetes induced delayed fetal skeletal ossification
title_full_unstemmed Protective effect of Cissus quadrangularis Linn. on diabetes induced delayed fetal skeletal ossification
title_short Protective effect of Cissus quadrangularis Linn. on diabetes induced delayed fetal skeletal ossification
title_sort protective effect of cissus quadrangularis linn. on diabetes induced delayed fetal skeletal ossification
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012358/
https://www.ncbi.nlm.nih.gov/pubmed/24812472
http://dx.doi.org/10.4103/0975-9476.128852
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