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Terminalia catappa attenuates urokinase-type plasminogen activator expression through Erk pathways in Hepatocellular carcinoma
BACKGROUND: The survival rate of malignant tumors, and especially hepatocellular carcinoma (HCC), has not improved primarily because of uncontrolled metastasis. In our previous studies, we have reported that Terminalia catappa leaf extract (TCE) exerts antimetastasis effects on HCC cells. However, t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012530/ https://www.ncbi.nlm.nih.gov/pubmed/24886639 http://dx.doi.org/10.1186/1472-6882-14-141 |
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author | Yeh, Chao-Bin Yu, Yung-Luen Lin, Chiao-Wen Chiou, Hui-Ling Hsieh, Ming-Ju Yang, Shun-Fa |
author_facet | Yeh, Chao-Bin Yu, Yung-Luen Lin, Chiao-Wen Chiou, Hui-Ling Hsieh, Ming-Ju Yang, Shun-Fa |
author_sort | Yeh, Chao-Bin |
collection | PubMed |
description | BACKGROUND: The survival rate of malignant tumors, and especially hepatocellular carcinoma (HCC), has not improved primarily because of uncontrolled metastasis. In our previous studies, we have reported that Terminalia catappa leaf extract (TCE) exerts antimetastasis effects on HCC cells. However, the molecular mechanisms of urokinase-type plasminogen activator (u-PA) in HCC metastasis have not been thoroughly investigated, and remain poorly understood. METHODS: The activities and protein levels of u-PA were determined by casein zymography and western blotting. Transcriptional levels of u-PA were detected by real-time PCR and promoter assays. RESULTS: We found that treatment of Huh7 cells with TCE significantly reduced the activities, protein levels and mRNA levels of u-PA. A chromatin immunoprecipitation (ChIP) assay showed that TCE inhibited the transcription protein of nuclear factors SP-1 and NF-κB. TCE also did inhibit the effects of u-PA by reducing the phosphorylation of ERK1/2 pathway. CONCLUSIONS: These results show that u-PA expression may be a potent therapeutic target in the TCE-mediated suppression of HCC metastasis. |
format | Online Article Text |
id | pubmed-4012530 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-40125302014-05-08 Terminalia catappa attenuates urokinase-type plasminogen activator expression through Erk pathways in Hepatocellular carcinoma Yeh, Chao-Bin Yu, Yung-Luen Lin, Chiao-Wen Chiou, Hui-Ling Hsieh, Ming-Ju Yang, Shun-Fa BMC Complement Altern Med Research Article BACKGROUND: The survival rate of malignant tumors, and especially hepatocellular carcinoma (HCC), has not improved primarily because of uncontrolled metastasis. In our previous studies, we have reported that Terminalia catappa leaf extract (TCE) exerts antimetastasis effects on HCC cells. However, the molecular mechanisms of urokinase-type plasminogen activator (u-PA) in HCC metastasis have not been thoroughly investigated, and remain poorly understood. METHODS: The activities and protein levels of u-PA were determined by casein zymography and western blotting. Transcriptional levels of u-PA were detected by real-time PCR and promoter assays. RESULTS: We found that treatment of Huh7 cells with TCE significantly reduced the activities, protein levels and mRNA levels of u-PA. A chromatin immunoprecipitation (ChIP) assay showed that TCE inhibited the transcription protein of nuclear factors SP-1 and NF-κB. TCE also did inhibit the effects of u-PA by reducing the phosphorylation of ERK1/2 pathway. CONCLUSIONS: These results show that u-PA expression may be a potent therapeutic target in the TCE-mediated suppression of HCC metastasis. BioMed Central 2014-04-30 /pmc/articles/PMC4012530/ /pubmed/24886639 http://dx.doi.org/10.1186/1472-6882-14-141 Text en Copyright © 2014 Yeh et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
spellingShingle | Research Article Yeh, Chao-Bin Yu, Yung-Luen Lin, Chiao-Wen Chiou, Hui-Ling Hsieh, Ming-Ju Yang, Shun-Fa Terminalia catappa attenuates urokinase-type plasminogen activator expression through Erk pathways in Hepatocellular carcinoma |
title | Terminalia catappa attenuates urokinase-type plasminogen activator expression through Erk pathways in Hepatocellular carcinoma |
title_full | Terminalia catappa attenuates urokinase-type plasminogen activator expression through Erk pathways in Hepatocellular carcinoma |
title_fullStr | Terminalia catappa attenuates urokinase-type plasminogen activator expression through Erk pathways in Hepatocellular carcinoma |
title_full_unstemmed | Terminalia catappa attenuates urokinase-type plasminogen activator expression through Erk pathways in Hepatocellular carcinoma |
title_short | Terminalia catappa attenuates urokinase-type plasminogen activator expression through Erk pathways in Hepatocellular carcinoma |
title_sort | terminalia catappa attenuates urokinase-type plasminogen activator expression through erk pathways in hepatocellular carcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012530/ https://www.ncbi.nlm.nih.gov/pubmed/24886639 http://dx.doi.org/10.1186/1472-6882-14-141 |
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