PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus

Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differ...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Wei, Song, Zhigang, Guan, Wencai, Liu, Yi, Zhang, Xiaonan, Xu, Lei, Li, Jianhua, Yuan, Zhenghong, Hu, Yunwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2014
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012804/
https://www.ncbi.nlm.nih.gov/pubmed/24751348
http://dx.doi.org/10.3201/eid2005.131364
_version_ 1782314974457102336
author Wang, Wei
Song, Zhigang
Guan, Wencai
Liu, Yi
Zhang, Xiaonan
Xu, Lei
Li, Jianhua
Yuan, Zhenghong
Hu, Yunwen
author_facet Wang, Wei
Song, Zhigang
Guan, Wencai
Liu, Yi
Zhang, Xiaonan
Xu, Lei
Li, Jianhua
Yuan, Zhenghong
Hu, Yunwen
author_sort Wang, Wei
collection PubMed
description Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens.
format Online
Article
Text
id pubmed-4012804
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Centers for Disease Control and Prevention
record_format MEDLINE/PubMed
spelling pubmed-40128042014-05-09 PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus Wang, Wei Song, Zhigang Guan, Wencai Liu, Yi Zhang, Xiaonan Xu, Lei Li, Jianhua Yuan, Zhenghong Hu, Yunwen Emerg Infect Dis Dispatch Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens. Centers for Disease Control and Prevention 2014-05 /pmc/articles/PMC4012804/ /pubmed/24751348 http://dx.doi.org/10.3201/eid2005.131364 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Wang, Wei
Song, Zhigang
Guan, Wencai
Liu, Yi
Zhang, Xiaonan
Xu, Lei
Li, Jianhua
Yuan, Zhenghong
Hu, Yunwen
PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus
title PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus
title_full PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus
title_fullStr PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus
title_full_unstemmed PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus
title_short PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus
title_sort pcr for detection of oseltamivir resistance mutation in influenza a(h7n9) virus
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012804/
https://www.ncbi.nlm.nih.gov/pubmed/24751348
http://dx.doi.org/10.3201/eid2005.131364
work_keys_str_mv AT wangwei pcrfordetectionofoseltamivirresistancemutationininfluenzaah7n9virus
AT songzhigang pcrfordetectionofoseltamivirresistancemutationininfluenzaah7n9virus
AT guanwencai pcrfordetectionofoseltamivirresistancemutationininfluenzaah7n9virus
AT liuyi pcrfordetectionofoseltamivirresistancemutationininfluenzaah7n9virus
AT zhangxiaonan pcrfordetectionofoseltamivirresistancemutationininfluenzaah7n9virus
AT xulei pcrfordetectionofoseltamivirresistancemutationininfluenzaah7n9virus
AT lijianhua pcrfordetectionofoseltamivirresistancemutationininfluenzaah7n9virus
AT yuanzhenghong pcrfordetectionofoseltamivirresistancemutationininfluenzaah7n9virus
AT huyunwen pcrfordetectionofoseltamivirresistancemutationininfluenzaah7n9virus

Ejemplares similares