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Secretome of the Biocontrol Agent Metarhizium anisopliae Induced by the Cuticle of the Cotton Pest Dysdercus peruvianus Reveals New Insights into Infection
[Image: see text] Metarhizium anisopliae is an entomopathogenic fungus that has evolved specialized strategies to infect insect hosts. Here we analyzed secreted proteins related to Dysdercus peruvianus infection. Using shotgun proteomics, abundance changes in 71 proteins were identified after exposu...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012838/ https://www.ncbi.nlm.nih.gov/pubmed/24702058 http://dx.doi.org/10.1021/pr401204y |
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author | Beys-da-Silva, Walter O. Santi, Lucélia Berger, Markus Calzolari, Diego Passos, Dario O. Guimarães, Jorge A. Moresco, James J. Yates, John R. |
author_facet | Beys-da-Silva, Walter O. Santi, Lucélia Berger, Markus Calzolari, Diego Passos, Dario O. Guimarães, Jorge A. Moresco, James J. Yates, John R. |
author_sort | Beys-da-Silva, Walter O. |
collection | PubMed |
description | [Image: see text] Metarhizium anisopliae is an entomopathogenic fungus that has evolved specialized strategies to infect insect hosts. Here we analyzed secreted proteins related to Dysdercus peruvianus infection. Using shotgun proteomics, abundance changes in 71 proteins were identified after exposure to host cuticle. Among these proteins were classical fungal effectors secreted by pathogens to degrade physical barriers and alter host physiology. These include lipolytic enzymes, Pr1A, B, C, I, and J proteases, ROS-related proteins, oxidorreductases, and signaling proteins. Protein interaction networks were generated postulating interesting candidates for further studies, including Pr1C, based on possible functional interactions. On the basis of these results, we propose that M. anisopliae is degrading host components and actively secreting proteins to manage the physiology of the host. Interestingly, the secretion of these factors occurs in the absence of a host response. The findings presented here are an important step in understanding the host–pathogen interaction and developing more efficient biocontrol of D. peruvianus by M. anisopliae. |
format | Online Article Text |
id | pubmed-4012838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-40128382015-04-07 Secretome of the Biocontrol Agent Metarhizium anisopliae Induced by the Cuticle of the Cotton Pest Dysdercus peruvianus Reveals New Insights into Infection Beys-da-Silva, Walter O. Santi, Lucélia Berger, Markus Calzolari, Diego Passos, Dario O. Guimarães, Jorge A. Moresco, James J. Yates, John R. J Proteome Res [Image: see text] Metarhizium anisopliae is an entomopathogenic fungus that has evolved specialized strategies to infect insect hosts. Here we analyzed secreted proteins related to Dysdercus peruvianus infection. Using shotgun proteomics, abundance changes in 71 proteins were identified after exposure to host cuticle. Among these proteins were classical fungal effectors secreted by pathogens to degrade physical barriers and alter host physiology. These include lipolytic enzymes, Pr1A, B, C, I, and J proteases, ROS-related proteins, oxidorreductases, and signaling proteins. Protein interaction networks were generated postulating interesting candidates for further studies, including Pr1C, based on possible functional interactions. On the basis of these results, we propose that M. anisopliae is degrading host components and actively secreting proteins to manage the physiology of the host. Interestingly, the secretion of these factors occurs in the absence of a host response. The findings presented here are an important step in understanding the host–pathogen interaction and developing more efficient biocontrol of D. peruvianus by M. anisopliae. American Chemical Society 2014-04-07 2014-05-02 /pmc/articles/PMC4012838/ /pubmed/24702058 http://dx.doi.org/10.1021/pr401204y Text en Copyright © 2014 American Chemical Society |
spellingShingle | Beys-da-Silva, Walter O. Santi, Lucélia Berger, Markus Calzolari, Diego Passos, Dario O. Guimarães, Jorge A. Moresco, James J. Yates, John R. Secretome of the Biocontrol Agent Metarhizium anisopliae Induced by the Cuticle of the Cotton Pest Dysdercus peruvianus Reveals New Insights into Infection |
title | Secretome of the Biocontrol
Agent Metarhizium
anisopliae Induced by the Cuticle of the Cotton Pest Dysdercus peruvianus Reveals New Insights into Infection |
title_full | Secretome of the Biocontrol
Agent Metarhizium
anisopliae Induced by the Cuticle of the Cotton Pest Dysdercus peruvianus Reveals New Insights into Infection |
title_fullStr | Secretome of the Biocontrol
Agent Metarhizium
anisopliae Induced by the Cuticle of the Cotton Pest Dysdercus peruvianus Reveals New Insights into Infection |
title_full_unstemmed | Secretome of the Biocontrol
Agent Metarhizium
anisopliae Induced by the Cuticle of the Cotton Pest Dysdercus peruvianus Reveals New Insights into Infection |
title_short | Secretome of the Biocontrol
Agent Metarhizium
anisopliae Induced by the Cuticle of the Cotton Pest Dysdercus peruvianus Reveals New Insights into Infection |
title_sort | secretome of the biocontrol
agent metarhizium
anisopliae induced by the cuticle of the cotton pest dysdercus peruvianus reveals new insights into infection |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4012838/ https://www.ncbi.nlm.nih.gov/pubmed/24702058 http://dx.doi.org/10.1021/pr401204y |
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