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Co-Expression of Foreign Proteins Tethered to HIV-1 Envelope Glycoprotein on the Cell Surface by Introducing an Intervening Second Membrane-Spanning Domain
The envelope glycoprotein (Env) of human immunodeficiency virus type I (HIV-1) mediates membrane fusion. To analyze the mechanism of HIV-1 Env-mediated membrane fusion, it is desirable to determine the expression level of Env on the cell surface. However, the quantification of Env by immunological s...
Autores principales: | Wang, Hongyun, Li, Xiao, Nakane, Shuhei, Liu, Shujun, Ishikawa, Hirohito, Iwamoto, Aikichi, Matsuda, Zene |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013048/ https://www.ncbi.nlm.nih.gov/pubmed/24804933 http://dx.doi.org/10.1371/journal.pone.0096790 |
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