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Demonstration of the Clathrin- and Caveolin-Mediated Endocytosis at the Maternal–Fetal Barrier in Mouse Placenta after Intravenous Administration of Gold Nanoparticles
Exposure to nanoparticles during pregnancy is a public concern, because nanoparticles may pass from the mother to the fetus across the placenta. The purpose of this study was to determine the possible translocation pathway of gold nanoparticles across the maternal–fetal barrier as well as the toxici...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013364/ https://www.ncbi.nlm.nih.gov/pubmed/24257253 http://dx.doi.org/10.1292/jvms.13-0512 |
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author | RATTANAPINYOPITUK, Kasem SHIMADA, Akinori MORITA, Takehito SAKURAI, Masashi ASANO, Atsushi HASEGAWA, Tatsuya INOUE, Kenichiro TAKANO, Hirohisa |
author_facet | RATTANAPINYOPITUK, Kasem SHIMADA, Akinori MORITA, Takehito SAKURAI, Masashi ASANO, Atsushi HASEGAWA, Tatsuya INOUE, Kenichiro TAKANO, Hirohisa |
author_sort | RATTANAPINYOPITUK, Kasem |
collection | PubMed |
description | Exposure to nanoparticles during pregnancy is a public concern, because nanoparticles may pass from the mother to the fetus across the placenta. The purpose of this study was to determine the possible translocation pathway of gold nanoparticles across the maternal–fetal barrier as well as the toxicity of intravenously administered gold nanoparticles to the placenta and fetus. Pregnant ICR mice were intravenously injected with 0.01% of 20- and 50-nm gold nanoparticle solutions on the 16th and 17th days of gestation. There was no sign of toxic damage to the placentas as well as maternal and fetal organs of the mice treated with 20- and 50-nm gold nanoparticles. ICP-MS analysis demonstrated significant amounts of gold deposited in the maternal livers and placentas, but no detectable level of gold in the fetal organs. However, electron microscopy demonstrated an increase of endocytic vesicles in the cytoplasm of syncytiotrophoblasts and fetal endothelial cells in the maternal–fetal barrier of mice treated with gold nanoparticles. Clathrin immunohistochemistry and immunoblotting showed increased immunoreactivity of clathrin protein in the placental tissues of mice treated with 20- and 50-nm gold nanoparticles; clathrin immunopositivity was observed in syncytiotrophoblasts and fetal endothelial cells. In contrast, caveolin-1 immunopositivity was observed exclusively in the fetal endothelium. These findings suggested that intravenous administration of gold nanoparticles may upregulate clathrin- and caveolin-mediated endocytosis at the maternal–fetal barrier in mouse placenta. |
format | Online Article Text |
id | pubmed-4013364 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40133642014-05-13 Demonstration of the Clathrin- and Caveolin-Mediated Endocytosis at the Maternal–Fetal Barrier in Mouse Placenta after Intravenous Administration of Gold Nanoparticles RATTANAPINYOPITUK, Kasem SHIMADA, Akinori MORITA, Takehito SAKURAI, Masashi ASANO, Atsushi HASEGAWA, Tatsuya INOUE, Kenichiro TAKANO, Hirohisa J Vet Med Sci Pathology Exposure to nanoparticles during pregnancy is a public concern, because nanoparticles may pass from the mother to the fetus across the placenta. The purpose of this study was to determine the possible translocation pathway of gold nanoparticles across the maternal–fetal barrier as well as the toxicity of intravenously administered gold nanoparticles to the placenta and fetus. Pregnant ICR mice were intravenously injected with 0.01% of 20- and 50-nm gold nanoparticle solutions on the 16th and 17th days of gestation. There was no sign of toxic damage to the placentas as well as maternal and fetal organs of the mice treated with 20- and 50-nm gold nanoparticles. ICP-MS analysis demonstrated significant amounts of gold deposited in the maternal livers and placentas, but no detectable level of gold in the fetal organs. However, electron microscopy demonstrated an increase of endocytic vesicles in the cytoplasm of syncytiotrophoblasts and fetal endothelial cells in the maternal–fetal barrier of mice treated with gold nanoparticles. Clathrin immunohistochemistry and immunoblotting showed increased immunoreactivity of clathrin protein in the placental tissues of mice treated with 20- and 50-nm gold nanoparticles; clathrin immunopositivity was observed in syncytiotrophoblasts and fetal endothelial cells. In contrast, caveolin-1 immunopositivity was observed exclusively in the fetal endothelium. These findings suggested that intravenous administration of gold nanoparticles may upregulate clathrin- and caveolin-mediated endocytosis at the maternal–fetal barrier in mouse placenta. The Japanese Society of Veterinary Science 2013-11-20 2014-03 /pmc/articles/PMC4013364/ /pubmed/24257253 http://dx.doi.org/10.1292/jvms.13-0512 Text en ©2014 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Pathology RATTANAPINYOPITUK, Kasem SHIMADA, Akinori MORITA, Takehito SAKURAI, Masashi ASANO, Atsushi HASEGAWA, Tatsuya INOUE, Kenichiro TAKANO, Hirohisa Demonstration of the Clathrin- and Caveolin-Mediated Endocytosis at the Maternal–Fetal Barrier in Mouse Placenta after Intravenous Administration of Gold Nanoparticles |
title | Demonstration of the Clathrin- and Caveolin-Mediated Endocytosis at the
Maternal–Fetal Barrier in Mouse Placenta after Intravenous Administration of Gold
Nanoparticles |
title_full | Demonstration of the Clathrin- and Caveolin-Mediated Endocytosis at the
Maternal–Fetal Barrier in Mouse Placenta after Intravenous Administration of Gold
Nanoparticles |
title_fullStr | Demonstration of the Clathrin- and Caveolin-Mediated Endocytosis at the
Maternal–Fetal Barrier in Mouse Placenta after Intravenous Administration of Gold
Nanoparticles |
title_full_unstemmed | Demonstration of the Clathrin- and Caveolin-Mediated Endocytosis at the
Maternal–Fetal Barrier in Mouse Placenta after Intravenous Administration of Gold
Nanoparticles |
title_short | Demonstration of the Clathrin- and Caveolin-Mediated Endocytosis at the
Maternal–Fetal Barrier in Mouse Placenta after Intravenous Administration of Gold
Nanoparticles |
title_sort | demonstration of the clathrin- and caveolin-mediated endocytosis at the
maternal–fetal barrier in mouse placenta after intravenous administration of gold
nanoparticles |
topic | Pathology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013364/ https://www.ncbi.nlm.nih.gov/pubmed/24257253 http://dx.doi.org/10.1292/jvms.13-0512 |
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