Screening for Intestinal Microflora Influencing Superoxide Dismutase Activity in Mouse Cecal Mucosa

We have suggested that intestinal microflora reduces the activity of the antioxidant enzyme superoxide dismutase (SOD) in the mouse cecal mucosa. In this study, gnotobiotic mice were used to examine the species of intestinal microflora influencing SOD activity in the cecal mucosa. The total SOD acti...

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Autores principales: DOBASHI, Yuu, ITOH, Kikuji, TOHEI, Atsushi, AMAO, Hiromi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2013
Materias:
Laboratory Animal Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013375/
https://www.ncbi.nlm.nih.gov/pubmed/24225363
http://dx.doi.org/10.1292/jvms.13-0329
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author DOBASHI, Yuu
ITOH, Kikuji
TOHEI, Atsushi
AMAO, Hiromi
author_facet DOBASHI, Yuu
ITOH, Kikuji
TOHEI, Atsushi
AMAO, Hiromi
author_sort DOBASHI, Yuu
collection PubMed
description We have suggested that intestinal microflora reduces the activity of the antioxidant enzyme superoxide dismutase (SOD) in the mouse cecal mucosa. In this study, gnotobiotic mice were used to examine the species of intestinal microflora influencing SOD activity in the cecal mucosa. The total SOD activity in the cecal mucosa of each germ-free (GF), gnotobiotic mouse with Escherichia coli, Lactobacillus and Bacteroides was significantly higher than that in the cecal mucosa of gnotobiotic mice with chloroform-treated feces (CHF), conventionalized (CVz) mice and conventional (CV) mice (P<0.05). In addition, CuZnSOD mRNA expression showed similar tendencies. Our results suggest that the antioxidant defense status in the cecal mucosa is influenced by CHF inoculation.
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spelling pubmed-40133752014-05-13 Screening for Intestinal Microflora Influencing Superoxide Dismutase Activity in Mouse Cecal Mucosa DOBASHI, Yuu ITOH, Kikuji TOHEI, Atsushi AMAO, Hiromi J Vet Med Sci Laboratory Animal Science We have suggested that intestinal microflora reduces the activity of the antioxidant enzyme superoxide dismutase (SOD) in the mouse cecal mucosa. In this study, gnotobiotic mice were used to examine the species of intestinal microflora influencing SOD activity in the cecal mucosa. The total SOD activity in the cecal mucosa of each germ-free (GF), gnotobiotic mouse with Escherichia coli, Lactobacillus and Bacteroides was significantly higher than that in the cecal mucosa of gnotobiotic mice with chloroform-treated feces (CHF), conventionalized (CVz) mice and conventional (CV) mice (P<0.05). In addition, CuZnSOD mRNA expression showed similar tendencies. Our results suggest that the antioxidant defense status in the cecal mucosa is influenced by CHF inoculation. The Japanese Society of Veterinary Science 2013-11-13 2014-03 /pmc/articles/PMC4013375/ /pubmed/24225363 http://dx.doi.org/10.1292/jvms.13-0329 Text en ©2014 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Laboratory Animal Science
DOBASHI, Yuu
ITOH, Kikuji
TOHEI, Atsushi
AMAO, Hiromi
Screening for Intestinal Microflora Influencing Superoxide Dismutase Activity in Mouse Cecal Mucosa
title Screening for Intestinal Microflora Influencing Superoxide Dismutase Activity in Mouse Cecal Mucosa
title_full Screening for Intestinal Microflora Influencing Superoxide Dismutase Activity in Mouse Cecal Mucosa
title_fullStr Screening for Intestinal Microflora Influencing Superoxide Dismutase Activity in Mouse Cecal Mucosa
title_full_unstemmed Screening for Intestinal Microflora Influencing Superoxide Dismutase Activity in Mouse Cecal Mucosa
title_short Screening for Intestinal Microflora Influencing Superoxide Dismutase Activity in Mouse Cecal Mucosa
title_sort screening for intestinal microflora influencing superoxide dismutase activity in mouse cecal mucosa
topic Laboratory Animal Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013375/
https://www.ncbi.nlm.nih.gov/pubmed/24225363
http://dx.doi.org/10.1292/jvms.13-0329
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