A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min

The polymerase chain reaction (PCR) has been widely used for diagnosis of infectious diseases of domestic animals. Rapid detection of respiratory pathogens of cattle is useful for making therapeutic decisions. Therefore, we developed a new genetic-based method called droplet-real-time PCR, which can...

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Autores principales: UEHARA, Masayuki, MATSUDA, Kazuyuki, SUGANO, Mitsutoshi, HONDA, Takayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2013
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013380/
https://www.ncbi.nlm.nih.gov/pubmed/24285011
http://dx.doi.org/10.1292/jvms.13-0357
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author UEHARA, Masayuki
MATSUDA, Kazuyuki
SUGANO, Mitsutoshi
HONDA, Takayuki
author_facet UEHARA, Masayuki
MATSUDA, Kazuyuki
SUGANO, Mitsutoshi
HONDA, Takayuki
author_sort UEHARA, Masayuki
collection PubMed
description The polymerase chain reaction (PCR) has been widely used for diagnosis of infectious diseases of domestic animals. Rapid detection of respiratory pathogens of cattle is useful for making therapeutic decisions. Therefore, we developed a new genetic-based method called droplet-real-time PCR, which can detect bovine respiratory syncytial virus (BRSV) within 10 min. Our droplet-real-time PCR markedly reduced the reaction time of reverse transcription-PCR while maintaining the same sensitivity as conventional real-time PCR, and it can be used as a rapid assay for detection of BRSV. Furthermore, our method is potentially applicable for rapid diagnosis of almost all infectious diseases, including highly pathogenic avian influenza virus.
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spelling pubmed-40133802014-05-13 A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min UEHARA, Masayuki MATSUDA, Kazuyuki SUGANO, Mitsutoshi HONDA, Takayuki J Vet Med Sci Virology The polymerase chain reaction (PCR) has been widely used for diagnosis of infectious diseases of domestic animals. Rapid detection of respiratory pathogens of cattle is useful for making therapeutic decisions. Therefore, we developed a new genetic-based method called droplet-real-time PCR, which can detect bovine respiratory syncytial virus (BRSV) within 10 min. Our droplet-real-time PCR markedly reduced the reaction time of reverse transcription-PCR while maintaining the same sensitivity as conventional real-time PCR, and it can be used as a rapid assay for detection of BRSV. Furthermore, our method is potentially applicable for rapid diagnosis of almost all infectious diseases, including highly pathogenic avian influenza virus. The Japanese Society of Veterinary Science 2013-11-28 2014-03 /pmc/articles/PMC4013380/ /pubmed/24285011 http://dx.doi.org/10.1292/jvms.13-0357 Text en ©2014 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Virology
UEHARA, Masayuki
MATSUDA, Kazuyuki
SUGANO, Mitsutoshi
HONDA, Takayuki
A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min
title A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min
title_full A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min
title_fullStr A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min
title_full_unstemmed A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min
title_short A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min
title_sort new high-speed droplet-real-time polymerase chain reaction method can detect bovine respiratory syncytial virus in less than 10 min
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013380/
https://www.ncbi.nlm.nih.gov/pubmed/24285011
http://dx.doi.org/10.1292/jvms.13-0357
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