Effect of β(2) -adrenergic receptor gene Arg16Gly polymorphisms on response to long-acting β(2)-agonist in Chinese Han asthmatic patients

BACKGROUND: To evaluate the effect of variation of the Arg16Gly polymorphism of the β(2)-adrenergic receptor gene on clinical response to salmeterol administered with fluticasone propionate in Chinese Han asthmatic patients. METHODS: Moderate persistent asthmatic patients (n = 62) currently receiving short-acting β(2)-agonists were administered twice-daily therapy with salmeterol/fluticasone propionate 50/250 μg in a single inhaler for 12 weeks, followed by a 2-to-4-day run-out period. Using direct DNA sequencing, five single nucleotide polymorphisms (SNPs) in the promoter and coding block regions of β(2)-adrenergic receptor gene were determined in 62 subjects and haplotypes were combined. RESULTS: There was sustained and significant improvement (p < 0.001) over baseline in all measures of asthma control in subjects receiving salmeterol and fluticasone, regardless of Arg16Gly genotype. However, there was no significant difference in the improvement among three genotypes (p > 0.05). Responses to salmeterol did not appear to be modified by haplotype pairs (p > 0.05). During the run-out period, all subjects had similar decreases in measures of asthma control, with no differences between genotypes (p > 0.05). CONCLUSIONS: Response to salmeterol does not vary with Arg16Gly polymorphisms after chronic dosing with inhaled corticosteroids in Chinese Han asthmatic patients.