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Terminal Protection of Small Molecule-Linked DNA for Small Molecule–Protein Interaction Assays

Methods for the detection of specific interactions between diverse proteins and various small-molecule ligands are of significant importance in understanding the mechanisms of many critical physiological processes of organisms. The techniques also represent a major avenue to drug screening, molecula...

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Detalles Bibliográficos
Autores principales: Hu, Cui, Wu, Zhan, Tang, Hao, Tang, Li-Juan, Yu, Ru-Qin, Jiang, Jian-Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013559/
https://www.ncbi.nlm.nih.gov/pubmed/24670475
http://dx.doi.org/10.3390/ijms15045221
Descripción
Sumario:Methods for the detection of specific interactions between diverse proteins and various small-molecule ligands are of significant importance in understanding the mechanisms of many critical physiological processes of organisms. The techniques also represent a major avenue to drug screening, molecular diagnostics, and public safety monitoring. Terminal protection assay of small molecule-linked DNA is a demonstrated novel methodology which has exhibited great potential for the development of simple, sensitive, specific and high-throughput methods for the detection of small molecule–protein interactions. Herein, we review the basic principle of terminal protection assay, the development of associated methods, and the signal amplification strategies adopted for performance improving in small molecule–protein interaction assay.