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Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization
Three calix[4]arene (Cal-4) derivatives which separately contain ethylester (1), carboxylic acid (2), and crownether (3) at the lower rim with a common reactive thiol at the upper rim were synthesized and constructed to self-assembled monolayers (SAMs) on Au films. After spectroscopic characterizati...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013577/ https://www.ncbi.nlm.nih.gov/pubmed/24690993 http://dx.doi.org/10.3390/ijms15045496 |
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author | Chen, Hongxia Liu, Feng Qi, Fangjie Koh, Kwangnak Wang, Keming |
author_facet | Chen, Hongxia Liu, Feng Qi, Fangjie Koh, Kwangnak Wang, Keming |
author_sort | Chen, Hongxia |
collection | PubMed |
description | Three calix[4]arene (Cal-4) derivatives which separately contain ethylester (1), carboxylic acid (2), and crownether (3) at the lower rim with a common reactive thiol at the upper rim were synthesized and constructed to self-assembled monolayers (SAMs) on Au films. After spectroscopic characterization of the monolayers, surface coverage and orientation of antibody immobilized on the Cal-4 derivative SAMs were studied by surface plasmon resonance (SPR) technique. Experimental results revealed that the antibody could be immobilized on the Cal-4 derivatives spontaneously. The orientation of absorbed antibody on the Cal-4 derivative SAMs is related to the SAM’s dipole moment. The possible orientations of the antibody immobilized on the Cal-4 derivative 1 SAM are lying-on or side-on, while on the Cal-4 derivative 2 and Cal-4 derivative 3 head-on and end-on respectively. These experimental results demonstrate the surface dipole moment of Cal-4 derivative appears to be an important factor to antibody orientation. Cal-4 derivatives are useful in developing site direct protein chips. |
format | Online Article Text |
id | pubmed-4013577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-40135772014-05-08 Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization Chen, Hongxia Liu, Feng Qi, Fangjie Koh, Kwangnak Wang, Keming Int J Mol Sci Article Three calix[4]arene (Cal-4) derivatives which separately contain ethylester (1), carboxylic acid (2), and crownether (3) at the lower rim with a common reactive thiol at the upper rim were synthesized and constructed to self-assembled monolayers (SAMs) on Au films. After spectroscopic characterization of the monolayers, surface coverage and orientation of antibody immobilized on the Cal-4 derivative SAMs were studied by surface plasmon resonance (SPR) technique. Experimental results revealed that the antibody could be immobilized on the Cal-4 derivatives spontaneously. The orientation of absorbed antibody on the Cal-4 derivative SAMs is related to the SAM’s dipole moment. The possible orientations of the antibody immobilized on the Cal-4 derivative 1 SAM are lying-on or side-on, while on the Cal-4 derivative 2 and Cal-4 derivative 3 head-on and end-on respectively. These experimental results demonstrate the surface dipole moment of Cal-4 derivative appears to be an important factor to antibody orientation. Cal-4 derivatives are useful in developing site direct protein chips. Molecular Diversity Preservation International (MDPI) 2014-03-31 /pmc/articles/PMC4013577/ /pubmed/24690993 http://dx.doi.org/10.3390/ijms15045496 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Chen, Hongxia Liu, Feng Qi, Fangjie Koh, Kwangnak Wang, Keming Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization |
title | Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization |
title_full | Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization |
title_fullStr | Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization |
title_full_unstemmed | Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization |
title_short | Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization |
title_sort | fabrication of calix[4]arene derivative monolayers to control orientation of antibody immobilization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013577/ https://www.ncbi.nlm.nih.gov/pubmed/24690993 http://dx.doi.org/10.3390/ijms15045496 |
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