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Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization

Three calix[4]arene (Cal-4) derivatives which separately contain ethylester (1), carboxylic acid (2), and crownether (3) at the lower rim with a common reactive thiol at the upper rim were synthesized and constructed to self-assembled monolayers (SAMs) on Au films. After spectroscopic characterizati...

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Autores principales: Chen, Hongxia, Liu, Feng, Qi, Fangjie, Koh, Kwangnak, Wang, Keming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013577/
https://www.ncbi.nlm.nih.gov/pubmed/24690993
http://dx.doi.org/10.3390/ijms15045496
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author Chen, Hongxia
Liu, Feng
Qi, Fangjie
Koh, Kwangnak
Wang, Keming
author_facet Chen, Hongxia
Liu, Feng
Qi, Fangjie
Koh, Kwangnak
Wang, Keming
author_sort Chen, Hongxia
collection PubMed
description Three calix[4]arene (Cal-4) derivatives which separately contain ethylester (1), carboxylic acid (2), and crownether (3) at the lower rim with a common reactive thiol at the upper rim were synthesized and constructed to self-assembled monolayers (SAMs) on Au films. After spectroscopic characterization of the monolayers, surface coverage and orientation of antibody immobilized on the Cal-4 derivative SAMs were studied by surface plasmon resonance (SPR) technique. Experimental results revealed that the antibody could be immobilized on the Cal-4 derivatives spontaneously. The orientation of absorbed antibody on the Cal-4 derivative SAMs is related to the SAM’s dipole moment. The possible orientations of the antibody immobilized on the Cal-4 derivative 1 SAM are lying-on or side-on, while on the Cal-4 derivative 2 and Cal-4 derivative 3 head-on and end-on respectively. These experimental results demonstrate the surface dipole moment of Cal-4 derivative appears to be an important factor to antibody orientation. Cal-4 derivatives are useful in developing site direct protein chips.
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spelling pubmed-40135772014-05-08 Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization Chen, Hongxia Liu, Feng Qi, Fangjie Koh, Kwangnak Wang, Keming Int J Mol Sci Article Three calix[4]arene (Cal-4) derivatives which separately contain ethylester (1), carboxylic acid (2), and crownether (3) at the lower rim with a common reactive thiol at the upper rim were synthesized and constructed to self-assembled monolayers (SAMs) on Au films. After spectroscopic characterization of the monolayers, surface coverage and orientation of antibody immobilized on the Cal-4 derivative SAMs were studied by surface plasmon resonance (SPR) technique. Experimental results revealed that the antibody could be immobilized on the Cal-4 derivatives spontaneously. The orientation of absorbed antibody on the Cal-4 derivative SAMs is related to the SAM’s dipole moment. The possible orientations of the antibody immobilized on the Cal-4 derivative 1 SAM are lying-on or side-on, while on the Cal-4 derivative 2 and Cal-4 derivative 3 head-on and end-on respectively. These experimental results demonstrate the surface dipole moment of Cal-4 derivative appears to be an important factor to antibody orientation. Cal-4 derivatives are useful in developing site direct protein chips. Molecular Diversity Preservation International (MDPI) 2014-03-31 /pmc/articles/PMC4013577/ /pubmed/24690993 http://dx.doi.org/10.3390/ijms15045496 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Chen, Hongxia
Liu, Feng
Qi, Fangjie
Koh, Kwangnak
Wang, Keming
Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization
title Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization
title_full Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization
title_fullStr Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization
title_full_unstemmed Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization
title_short Fabrication of Calix[4]arene Derivative Monolayers to Control Orientation of Antibody Immobilization
title_sort fabrication of calix[4]arene derivative monolayers to control orientation of antibody immobilization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013577/
https://www.ncbi.nlm.nih.gov/pubmed/24690993
http://dx.doi.org/10.3390/ijms15045496
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