Cargando…
The Activity of Nodules of the Supernodulating Mutant Mt(sunn) Is not Limited by Photosynthesis under Optimal Growth Conditions
Legumes match the nodule number to the N demand of the plant. When a mutation in the regulatory mechanism deprives the plant of that ability, an excessive number of nodules are formed. These mutants show low productivity in the fields, mainly due to the high carbon burden caused through the necessit...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013613/ https://www.ncbi.nlm.nih.gov/pubmed/24727372 http://dx.doi.org/10.3390/ijms15046031 |
_version_ | 1782315085028392960 |
---|---|
author | Cabeza, Ricardo A. Lingner, Annika Liese, Rebecca Sulieman, Saad Senbayram, Mehmet Tränkner, Merle Dittert, Klaus Schulze, Joachim |
author_facet | Cabeza, Ricardo A. Lingner, Annika Liese, Rebecca Sulieman, Saad Senbayram, Mehmet Tränkner, Merle Dittert, Klaus Schulze, Joachim |
author_sort | Cabeza, Ricardo A. |
collection | PubMed |
description | Legumes match the nodule number to the N demand of the plant. When a mutation in the regulatory mechanism deprives the plant of that ability, an excessive number of nodules are formed. These mutants show low productivity in the fields, mainly due to the high carbon burden caused through the necessity to supply numerous nodules. The objective of this study was to clarify whether through optimal conditions for growth and CO(2) assimilation a higher nodule activity of a supernodulating mutant of Medicago truncatula (M. truncatula) can be induced. Several experimental approaches reveal that under the conditions of our experiments, the nitrogen fixation of the supernodulating mutant, designated as sunn (super numeric nodules), was not limited by photosynthesis. Higher specific nitrogen fixation activity could not be induced through short- or long-term increases in CO(2) assimilation around shoots. Furthermore, a whole plant P depletion induced a decline in nitrogen fixation, however this decline did not occur significantly earlier in sunn plants, nor was it more intense compared to the wild-type. However, a distinctly different pattern of nitrogen fixation during the day/night cycles of the experiment indicates that the control of N(2) fixing activity of the large number of nodules is an additional problem for the productivity of supernodulating mutants. |
format | Online Article Text |
id | pubmed-4013613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-40136132014-05-08 The Activity of Nodules of the Supernodulating Mutant Mt(sunn) Is not Limited by Photosynthesis under Optimal Growth Conditions Cabeza, Ricardo A. Lingner, Annika Liese, Rebecca Sulieman, Saad Senbayram, Mehmet Tränkner, Merle Dittert, Klaus Schulze, Joachim Int J Mol Sci Article Legumes match the nodule number to the N demand of the plant. When a mutation in the regulatory mechanism deprives the plant of that ability, an excessive number of nodules are formed. These mutants show low productivity in the fields, mainly due to the high carbon burden caused through the necessity to supply numerous nodules. The objective of this study was to clarify whether through optimal conditions for growth and CO(2) assimilation a higher nodule activity of a supernodulating mutant of Medicago truncatula (M. truncatula) can be induced. Several experimental approaches reveal that under the conditions of our experiments, the nitrogen fixation of the supernodulating mutant, designated as sunn (super numeric nodules), was not limited by photosynthesis. Higher specific nitrogen fixation activity could not be induced through short- or long-term increases in CO(2) assimilation around shoots. Furthermore, a whole plant P depletion induced a decline in nitrogen fixation, however this decline did not occur significantly earlier in sunn plants, nor was it more intense compared to the wild-type. However, a distinctly different pattern of nitrogen fixation during the day/night cycles of the experiment indicates that the control of N(2) fixing activity of the large number of nodules is an additional problem for the productivity of supernodulating mutants. Molecular Diversity Preservation International (MDPI) 2014-04-10 /pmc/articles/PMC4013613/ /pubmed/24727372 http://dx.doi.org/10.3390/ijms15046031 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Cabeza, Ricardo A. Lingner, Annika Liese, Rebecca Sulieman, Saad Senbayram, Mehmet Tränkner, Merle Dittert, Klaus Schulze, Joachim The Activity of Nodules of the Supernodulating Mutant Mt(sunn) Is not Limited by Photosynthesis under Optimal Growth Conditions |
title | The Activity of Nodules of the Supernodulating Mutant Mt(sunn) Is not Limited by Photosynthesis under Optimal Growth Conditions |
title_full | The Activity of Nodules of the Supernodulating Mutant Mt(sunn) Is not Limited by Photosynthesis under Optimal Growth Conditions |
title_fullStr | The Activity of Nodules of the Supernodulating Mutant Mt(sunn) Is not Limited by Photosynthesis under Optimal Growth Conditions |
title_full_unstemmed | The Activity of Nodules of the Supernodulating Mutant Mt(sunn) Is not Limited by Photosynthesis under Optimal Growth Conditions |
title_short | The Activity of Nodules of the Supernodulating Mutant Mt(sunn) Is not Limited by Photosynthesis under Optimal Growth Conditions |
title_sort | activity of nodules of the supernodulating mutant mt(sunn) is not limited by photosynthesis under optimal growth conditions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013613/ https://www.ncbi.nlm.nih.gov/pubmed/24727372 http://dx.doi.org/10.3390/ijms15046031 |
work_keys_str_mv | AT cabezaricardoa theactivityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT lingnerannika theactivityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT lieserebecca theactivityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT suliemansaad theactivityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT senbayrammehmet theactivityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT tranknermerle theactivityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT dittertklaus theactivityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT schulzejoachim theactivityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT cabezaricardoa activityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT lingnerannika activityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT lieserebecca activityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT suliemansaad activityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT senbayrammehmet activityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT tranknermerle activityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT dittertklaus activityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions AT schulzejoachim activityofnodulesofthesupernodulatingmutantmtsunnisnotlimitedbyphotosynthesisunderoptimalgrowthconditions |