Cargando…

The lethal giant larvae Gene in Tribolium castaneum: Molecular Properties and Roles in Larval and Pupal Development as Revealed by RNA Interference

We identified and characterized the TcLgl gene putatively encoding lethal giant larvae (Lgl) protein from the red flour beetle (Tribolium castaneum). Analyses of developmental stage and tissue-specific expression patterns revealed that TcLgl was constitutively expressed. To examine the role of TcLgl...

Descripción completa

Detalles Bibliográficos
Autores principales: Xiao, Da, Liang, Xiao, Gao, Xiwu, Yao, Jianxiu, Zhu, Kun Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013667/
https://www.ncbi.nlm.nih.gov/pubmed/24758930
http://dx.doi.org/10.3390/ijms15046880
_version_ 1782315097423609856
author Xiao, Da
Liang, Xiao
Gao, Xiwu
Yao, Jianxiu
Zhu, Kun Yan
author_facet Xiao, Da
Liang, Xiao
Gao, Xiwu
Yao, Jianxiu
Zhu, Kun Yan
author_sort Xiao, Da
collection PubMed
description We identified and characterized the TcLgl gene putatively encoding lethal giant larvae (Lgl) protein from the red flour beetle (Tribolium castaneum). Analyses of developmental stage and tissue-specific expression patterns revealed that TcLgl was constitutively expressed. To examine the role of TcLgl in insect development, RNA interference was performed in early (1-day) larvae, late (20-day) larvae, and early (1-day) pupae. The early larvae injected with double-stranded RNA of TcLgl (dsTcLgl) at 100, 200, and 400 ng/larva failed to pupate, and 100% mortality was achieved within 20 days after the injection or before the pupation. The late larvae injected with dsTcLgl at these doses reduced the pupation rates to only 50.3%, 36.0%, and 18.2%, respectively. The un-pupated larvae gradually died after one week, and visually unaffected pupae failed to emerge into adults and died during the pupal stage. Similarly, when early pupae were injected with dsTcLgl at these doses, the normal eclosion rates were reduced to only 22.5%, 18.0%, and 11.2%, respectively, on day 7 after the injection, and all the adults with abnormal eclosion died in two days after the eclosion. These results indicate that TcLgl plays an essential role in insect development, especially during their metamorphosis.
format Online
Article
Text
id pubmed-4013667
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-40136672014-05-08 The lethal giant larvae Gene in Tribolium castaneum: Molecular Properties and Roles in Larval and Pupal Development as Revealed by RNA Interference Xiao, Da Liang, Xiao Gao, Xiwu Yao, Jianxiu Zhu, Kun Yan Int J Mol Sci Article We identified and characterized the TcLgl gene putatively encoding lethal giant larvae (Lgl) protein from the red flour beetle (Tribolium castaneum). Analyses of developmental stage and tissue-specific expression patterns revealed that TcLgl was constitutively expressed. To examine the role of TcLgl in insect development, RNA interference was performed in early (1-day) larvae, late (20-day) larvae, and early (1-day) pupae. The early larvae injected with double-stranded RNA of TcLgl (dsTcLgl) at 100, 200, and 400 ng/larva failed to pupate, and 100% mortality was achieved within 20 days after the injection or before the pupation. The late larvae injected with dsTcLgl at these doses reduced the pupation rates to only 50.3%, 36.0%, and 18.2%, respectively. The un-pupated larvae gradually died after one week, and visually unaffected pupae failed to emerge into adults and died during the pupal stage. Similarly, when early pupae were injected with dsTcLgl at these doses, the normal eclosion rates were reduced to only 22.5%, 18.0%, and 11.2%, respectively, on day 7 after the injection, and all the adults with abnormal eclosion died in two days after the eclosion. These results indicate that TcLgl plays an essential role in insect development, especially during their metamorphosis. Molecular Diversity Preservation International (MDPI) 2014-04-22 /pmc/articles/PMC4013667/ /pubmed/24758930 http://dx.doi.org/10.3390/ijms15046880 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Xiao, Da
Liang, Xiao
Gao, Xiwu
Yao, Jianxiu
Zhu, Kun Yan
The lethal giant larvae Gene in Tribolium castaneum: Molecular Properties and Roles in Larval and Pupal Development as Revealed by RNA Interference
title The lethal giant larvae Gene in Tribolium castaneum: Molecular Properties and Roles in Larval and Pupal Development as Revealed by RNA Interference
title_full The lethal giant larvae Gene in Tribolium castaneum: Molecular Properties and Roles in Larval and Pupal Development as Revealed by RNA Interference
title_fullStr The lethal giant larvae Gene in Tribolium castaneum: Molecular Properties and Roles in Larval and Pupal Development as Revealed by RNA Interference
title_full_unstemmed The lethal giant larvae Gene in Tribolium castaneum: Molecular Properties and Roles in Larval and Pupal Development as Revealed by RNA Interference
title_short The lethal giant larvae Gene in Tribolium castaneum: Molecular Properties and Roles in Larval and Pupal Development as Revealed by RNA Interference
title_sort lethal giant larvae gene in tribolium castaneum: molecular properties and roles in larval and pupal development as revealed by rna interference
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013667/
https://www.ncbi.nlm.nih.gov/pubmed/24758930
http://dx.doi.org/10.3390/ijms15046880
work_keys_str_mv AT xiaoda thelethalgiantlarvaegeneintriboliumcastaneummolecularpropertiesandrolesinlarvalandpupaldevelopmentasrevealedbyrnainterference
AT liangxiao thelethalgiantlarvaegeneintriboliumcastaneummolecularpropertiesandrolesinlarvalandpupaldevelopmentasrevealedbyrnainterference
AT gaoxiwu thelethalgiantlarvaegeneintriboliumcastaneummolecularpropertiesandrolesinlarvalandpupaldevelopmentasrevealedbyrnainterference
AT yaojianxiu thelethalgiantlarvaegeneintriboliumcastaneummolecularpropertiesandrolesinlarvalandpupaldevelopmentasrevealedbyrnainterference
AT zhukunyan thelethalgiantlarvaegeneintriboliumcastaneummolecularpropertiesandrolesinlarvalandpupaldevelopmentasrevealedbyrnainterference
AT xiaoda lethalgiantlarvaegeneintriboliumcastaneummolecularpropertiesandrolesinlarvalandpupaldevelopmentasrevealedbyrnainterference
AT liangxiao lethalgiantlarvaegeneintriboliumcastaneummolecularpropertiesandrolesinlarvalandpupaldevelopmentasrevealedbyrnainterference
AT gaoxiwu lethalgiantlarvaegeneintriboliumcastaneummolecularpropertiesandrolesinlarvalandpupaldevelopmentasrevealedbyrnainterference
AT yaojianxiu lethalgiantlarvaegeneintriboliumcastaneummolecularpropertiesandrolesinlarvalandpupaldevelopmentasrevealedbyrnainterference
AT zhukunyan lethalgiantlarvaegeneintriboliumcastaneummolecularpropertiesandrolesinlarvalandpupaldevelopmentasrevealedbyrnainterference