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LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells
Osteosarcoma (OS), also known as osteogenic sarcoma, is the most common primary malignancy of bone tumor in children and adolescents. However, its underlying molecular pathogenesis is still only vaguely understood. Recently, LIM mineralization protein-1 (LMP-1) was reported to be an essential positi...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013677/ https://www.ncbi.nlm.nih.gov/pubmed/24762763 http://dx.doi.org/10.3390/ijms15047037 |
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author | Liu, Huiwen Huang, Lu Zhang, Zhongzu Zhang, Zhanming Yu, Zhiming Chen, Xiang Chen, Zhuo Zen, Yongping Yang, Dong Han, Zhimin Shu, Yong Dai, Min Cao, Kai |
author_facet | Liu, Huiwen Huang, Lu Zhang, Zhongzu Zhang, Zhanming Yu, Zhiming Chen, Xiang Chen, Zhuo Zen, Yongping Yang, Dong Han, Zhimin Shu, Yong Dai, Min Cao, Kai |
author_sort | Liu, Huiwen |
collection | PubMed |
description | Osteosarcoma (OS), also known as osteogenic sarcoma, is the most common primary malignancy of bone tumor in children and adolescents. However, its underlying molecular pathogenesis is still only vaguely understood. Recently, LIM mineralization protein-1 (LMP-1) was reported to be an essential positive regulator of osteoblast differentiation. In the present study, we found that the expression of LMP-1 is downregulated in OS tissues compared with adjacent normal tissues. Moreover, we restored the expression of LMP-1 through a recombinant adenovirus. Overexpression of LMP-1 inhibited cell proliferation and invasion, arrested cell cycle progression, and induced apoptosis in vitro. Finally, ectopic LMP-1 expression suppressed the expression of Runx2 and BMP-2 in OS cells. These data demonstrate that LMP-1 is an essential tumor suppressor in the OS pathological process, which will provide a new opportunity for discovering and identifying novel effective treatment strategies. |
format | Online Article Text |
id | pubmed-4013677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-40136772014-05-08 LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells Liu, Huiwen Huang, Lu Zhang, Zhongzu Zhang, Zhanming Yu, Zhiming Chen, Xiang Chen, Zhuo Zen, Yongping Yang, Dong Han, Zhimin Shu, Yong Dai, Min Cao, Kai Int J Mol Sci Article Osteosarcoma (OS), also known as osteogenic sarcoma, is the most common primary malignancy of bone tumor in children and adolescents. However, its underlying molecular pathogenesis is still only vaguely understood. Recently, LIM mineralization protein-1 (LMP-1) was reported to be an essential positive regulator of osteoblast differentiation. In the present study, we found that the expression of LMP-1 is downregulated in OS tissues compared with adjacent normal tissues. Moreover, we restored the expression of LMP-1 through a recombinant adenovirus. Overexpression of LMP-1 inhibited cell proliferation and invasion, arrested cell cycle progression, and induced apoptosis in vitro. Finally, ectopic LMP-1 expression suppressed the expression of Runx2 and BMP-2 in OS cells. These data demonstrate that LMP-1 is an essential tumor suppressor in the OS pathological process, which will provide a new opportunity for discovering and identifying novel effective treatment strategies. Molecular Diversity Preservation International (MDPI) 2014-04-23 /pmc/articles/PMC4013677/ /pubmed/24762763 http://dx.doi.org/10.3390/ijms15047037 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Liu, Huiwen Huang, Lu Zhang, Zhongzu Zhang, Zhanming Yu, Zhiming Chen, Xiang Chen, Zhuo Zen, Yongping Yang, Dong Han, Zhimin Shu, Yong Dai, Min Cao, Kai LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells |
title | LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells |
title_full | LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells |
title_fullStr | LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells |
title_full_unstemmed | LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells |
title_short | LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells |
title_sort | lim mineralization protein-1 inhibits the malignant phenotypes of human osteosarcoma cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013677/ https://www.ncbi.nlm.nih.gov/pubmed/24762763 http://dx.doi.org/10.3390/ijms15047037 |
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