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LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells

Osteosarcoma (OS), also known as osteogenic sarcoma, is the most common primary malignancy of bone tumor in children and adolescents. However, its underlying molecular pathogenesis is still only vaguely understood. Recently, LIM mineralization protein-1 (LMP-1) was reported to be an essential positi...

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Autores principales: Liu, Huiwen, Huang, Lu, Zhang, Zhongzu, Zhang, Zhanming, Yu, Zhiming, Chen, Xiang, Chen, Zhuo, Zen, Yongping, Yang, Dong, Han, Zhimin, Shu, Yong, Dai, Min, Cao, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013677/
https://www.ncbi.nlm.nih.gov/pubmed/24762763
http://dx.doi.org/10.3390/ijms15047037
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author Liu, Huiwen
Huang, Lu
Zhang, Zhongzu
Zhang, Zhanming
Yu, Zhiming
Chen, Xiang
Chen, Zhuo
Zen, Yongping
Yang, Dong
Han, Zhimin
Shu, Yong
Dai, Min
Cao, Kai
author_facet Liu, Huiwen
Huang, Lu
Zhang, Zhongzu
Zhang, Zhanming
Yu, Zhiming
Chen, Xiang
Chen, Zhuo
Zen, Yongping
Yang, Dong
Han, Zhimin
Shu, Yong
Dai, Min
Cao, Kai
author_sort Liu, Huiwen
collection PubMed
description Osteosarcoma (OS), also known as osteogenic sarcoma, is the most common primary malignancy of bone tumor in children and adolescents. However, its underlying molecular pathogenesis is still only vaguely understood. Recently, LIM mineralization protein-1 (LMP-1) was reported to be an essential positive regulator of osteoblast differentiation. In the present study, we found that the expression of LMP-1 is downregulated in OS tissues compared with adjacent normal tissues. Moreover, we restored the expression of LMP-1 through a recombinant adenovirus. Overexpression of LMP-1 inhibited cell proliferation and invasion, arrested cell cycle progression, and induced apoptosis in vitro. Finally, ectopic LMP-1 expression suppressed the expression of Runx2 and BMP-2 in OS cells. These data demonstrate that LMP-1 is an essential tumor suppressor in the OS pathological process, which will provide a new opportunity for discovering and identifying novel effective treatment strategies.
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spelling pubmed-40136772014-05-08 LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells Liu, Huiwen Huang, Lu Zhang, Zhongzu Zhang, Zhanming Yu, Zhiming Chen, Xiang Chen, Zhuo Zen, Yongping Yang, Dong Han, Zhimin Shu, Yong Dai, Min Cao, Kai Int J Mol Sci Article Osteosarcoma (OS), also known as osteogenic sarcoma, is the most common primary malignancy of bone tumor in children and adolescents. However, its underlying molecular pathogenesis is still only vaguely understood. Recently, LIM mineralization protein-1 (LMP-1) was reported to be an essential positive regulator of osteoblast differentiation. In the present study, we found that the expression of LMP-1 is downregulated in OS tissues compared with adjacent normal tissues. Moreover, we restored the expression of LMP-1 through a recombinant adenovirus. Overexpression of LMP-1 inhibited cell proliferation and invasion, arrested cell cycle progression, and induced apoptosis in vitro. Finally, ectopic LMP-1 expression suppressed the expression of Runx2 and BMP-2 in OS cells. These data demonstrate that LMP-1 is an essential tumor suppressor in the OS pathological process, which will provide a new opportunity for discovering and identifying novel effective treatment strategies. Molecular Diversity Preservation International (MDPI) 2014-04-23 /pmc/articles/PMC4013677/ /pubmed/24762763 http://dx.doi.org/10.3390/ijms15047037 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Liu, Huiwen
Huang, Lu
Zhang, Zhongzu
Zhang, Zhanming
Yu, Zhiming
Chen, Xiang
Chen, Zhuo
Zen, Yongping
Yang, Dong
Han, Zhimin
Shu, Yong
Dai, Min
Cao, Kai
LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells
title LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells
title_full LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells
title_fullStr LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells
title_full_unstemmed LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells
title_short LIM Mineralization Protein-1 Inhibits the Malignant Phenotypes of Human Osteosarcoma Cells
title_sort lim mineralization protein-1 inhibits the malignant phenotypes of human osteosarcoma cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013677/
https://www.ncbi.nlm.nih.gov/pubmed/24762763
http://dx.doi.org/10.3390/ijms15047037
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