Evaluation of the Usefulness of a PCR Assay Performed at a Clinical Laboratory for the Diagnosis of Respiratory Disease Induced by Equine Herpesvirus Type 1 in the Field

A PCR assay for the diagnosis of respiratory disease induced by equine herpesvirus type 1 (EHV-1) was performed at the clinical laboratory in the Racehorse Clinic of the Ritto Training Center of the Japan Racing Association from December 2007 to March 2008. The assay was performed without the troubl...

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Autores principales: OHTA, Minoru, NEMOTO, Manabu, TSUJIMURA, Koji, KONDO, Takashi, MATSUMURA, Tomio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Equine Science 2011
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013993/
https://www.ncbi.nlm.nih.gov/pubmed/24833987
http://dx.doi.org/10.1294/jes.22.53
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author OHTA, Minoru
NEMOTO, Manabu
TSUJIMURA, Koji
KONDO, Takashi
MATSUMURA, Tomio
author_facet OHTA, Minoru
NEMOTO, Manabu
TSUJIMURA, Koji
KONDO, Takashi
MATSUMURA, Tomio
author_sort OHTA, Minoru
collection PubMed
description A PCR assay for the diagnosis of respiratory disease induced by equine herpesvirus type 1 (EHV-1) was performed at the clinical laboratory in the Racehorse Clinic of the Ritto Training Center of the Japan Racing Association from December 2007 to March 2008. The assay was performed without the trouble of contamination throughout the study and its turnaround time was approximately 6 hr. The PCR detection rates of EHV-1 among seroconverted horses were 22.2% for nasal swabs and 33.3% for blood samples. However, EHV-1 DNA was also detected in horses without seroconversion at a low rate. These results indicated that the PCR assay should be used as an adjunct method, but would help to make an early diagnosis of EHV-1 infection.
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spelling pubmed-40139932014-05-15 Evaluation of the Usefulness of a PCR Assay Performed at a Clinical Laboratory for the Diagnosis of Respiratory Disease Induced by Equine Herpesvirus Type 1 in the Field OHTA, Minoru NEMOTO, Manabu TSUJIMURA, Koji KONDO, Takashi MATSUMURA, Tomio J Equine Sci Note A PCR assay for the diagnosis of respiratory disease induced by equine herpesvirus type 1 (EHV-1) was performed at the clinical laboratory in the Racehorse Clinic of the Ritto Training Center of the Japan Racing Association from December 2007 to March 2008. The assay was performed without the trouble of contamination throughout the study and its turnaround time was approximately 6 hr. The PCR detection rates of EHV-1 among seroconverted horses were 22.2% for nasal swabs and 33.3% for blood samples. However, EHV-1 DNA was also detected in horses without seroconversion at a low rate. These results indicated that the PCR assay should be used as an adjunct method, but would help to make an early diagnosis of EHV-1 infection. The Japanese Society of Equine Science 2011-10-25 2011 /pmc/articles/PMC4013993/ /pubmed/24833987 http://dx.doi.org/10.1294/jes.22.53 Text en 2011 The Japanese Society of Equine Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Note
OHTA, Minoru
NEMOTO, Manabu
TSUJIMURA, Koji
KONDO, Takashi
MATSUMURA, Tomio
Evaluation of the Usefulness of a PCR Assay Performed at a Clinical Laboratory for the Diagnosis of Respiratory Disease Induced by Equine Herpesvirus Type 1 in the Field
title Evaluation of the Usefulness of a PCR Assay Performed at a Clinical Laboratory for the Diagnosis of Respiratory Disease Induced by Equine Herpesvirus Type 1 in the Field
title_full Evaluation of the Usefulness of a PCR Assay Performed at a Clinical Laboratory for the Diagnosis of Respiratory Disease Induced by Equine Herpesvirus Type 1 in the Field
title_fullStr Evaluation of the Usefulness of a PCR Assay Performed at a Clinical Laboratory for the Diagnosis of Respiratory Disease Induced by Equine Herpesvirus Type 1 in the Field
title_full_unstemmed Evaluation of the Usefulness of a PCR Assay Performed at a Clinical Laboratory for the Diagnosis of Respiratory Disease Induced by Equine Herpesvirus Type 1 in the Field
title_short Evaluation of the Usefulness of a PCR Assay Performed at a Clinical Laboratory for the Diagnosis of Respiratory Disease Induced by Equine Herpesvirus Type 1 in the Field
title_sort evaluation of the usefulness of a pcr assay performed at a clinical laboratory for the diagnosis of respiratory disease induced by equine herpesvirus type 1 in the field
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4013993/
https://www.ncbi.nlm.nih.gov/pubmed/24833987
http://dx.doi.org/10.1294/jes.22.53
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