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IR laser-induced protein crystal transformation

A method and the design of instrumentation, and its preliminary practical realisation, including test experiments, with the object of inducing phase changes of biomolecular crystals by controlled dehydration through heating with infrared (IR) light are described. The aim is to generate and select cr...

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Autores principales: Kiefersauer, Reiner, Grandl, Brigitte, Krapp, Stephan, Huber, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014118/
https://www.ncbi.nlm.nih.gov/pubmed/24816092
http://dx.doi.org/10.1107/S1399004714002223
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author Kiefersauer, Reiner
Grandl, Brigitte
Krapp, Stephan
Huber, Robert
author_facet Kiefersauer, Reiner
Grandl, Brigitte
Krapp, Stephan
Huber, Robert
author_sort Kiefersauer, Reiner
collection PubMed
description A method and the design of instrumentation, and its preliminary practical realisation, including test experiments, with the object of inducing phase changes of biomolecular crystals by controlled dehydration through heating with infrared (IR) light are described. The aim is to generate and select crystalline phases through transformation in the solid state which have improved order (higher resolution in X-ray diffraction experiments) and reduced mosaic spread (more uniformly aligned mosaic blocks) for diffraction data collection and analysis. The crystal is heated by pulsed and/or constant IR laser irradiation. Loss of crystal water following heating and its reabsorption through equilibration with the environment is measured optically by a video system. Heating proved superior to traditional controlled dehydration by humidity change for the test cases CODH (carbon monoxide dehydrogenase) and CLK2 (a protein kinase). Heating with IR light is experimentally simple and offers an exploration of a much broader parameter space than the traditional method, as it allows the option of varying the rate of phase changes through modification of the IR pulse strength, width and repeat frequency. It impacts the crystal instantaneously, isotropically and homogeneously, and is therefore expected to cause less mechanical stress.
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spelling pubmed-40141182014-06-05 IR laser-induced protein crystal transformation Kiefersauer, Reiner Grandl, Brigitte Krapp, Stephan Huber, Robert Acta Crystallogr D Biol Crystallogr Research Papers A method and the design of instrumentation, and its preliminary practical realisation, including test experiments, with the object of inducing phase changes of biomolecular crystals by controlled dehydration through heating with infrared (IR) light are described. The aim is to generate and select crystalline phases through transformation in the solid state which have improved order (higher resolution in X-ray diffraction experiments) and reduced mosaic spread (more uniformly aligned mosaic blocks) for diffraction data collection and analysis. The crystal is heated by pulsed and/or constant IR laser irradiation. Loss of crystal water following heating and its reabsorption through equilibration with the environment is measured optically by a video system. Heating proved superior to traditional controlled dehydration by humidity change for the test cases CODH (carbon monoxide dehydrogenase) and CLK2 (a protein kinase). Heating with IR light is experimentally simple and offers an exploration of a much broader parameter space than the traditional method, as it allows the option of varying the rate of phase changes through modification of the IR pulse strength, width and repeat frequency. It impacts the crystal instantaneously, isotropically and homogeneously, and is therefore expected to cause less mechanical stress. International Union of Crystallography 2014-04-26 /pmc/articles/PMC4014118/ /pubmed/24816092 http://dx.doi.org/10.1107/S1399004714002223 Text en © Kiefersauer et al. 2014 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Kiefersauer, Reiner
Grandl, Brigitte
Krapp, Stephan
Huber, Robert
IR laser-induced protein crystal transformation
title IR laser-induced protein crystal transformation
title_full IR laser-induced protein crystal transformation
title_fullStr IR laser-induced protein crystal transformation
title_full_unstemmed IR laser-induced protein crystal transformation
title_short IR laser-induced protein crystal transformation
title_sort ir laser-induced protein crystal transformation
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014118/
https://www.ncbi.nlm.nih.gov/pubmed/24816092
http://dx.doi.org/10.1107/S1399004714002223
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