Exploiting fast detectors to enter a new dimension in room-temperature crystallography

A departure from a linear or an exponential intensity decay in the diffracting power of protein crystals as a function of absorbed dose is reported. The observation of a lag phase raises the possibility of collecting significantly more data from crystals held at room temperature before an intolerabl...

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Detalles Bibliográficos
Autores principales: Owen, Robin L., Paterson, Neil, Axford, Danny, Aishima, Jun, Schulze-Briese, Clemens, Ren, Jingshan, Fry, Elizabeth E., Stuart, David I., Evans, Gwyndaf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2014
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014120/
https://www.ncbi.nlm.nih.gov/pubmed/24816094
http://dx.doi.org/10.1107/S1399004714005379
Descripción
Sumario:A departure from a linear or an exponential intensity decay in the diffracting power of protein crystals as a function of absorbed dose is reported. The observation of a lag phase raises the possibility of collecting significantly more data from crystals held at room temperature before an intolerable intensity decay is reached. A simple model accounting for the form of the intensity decay is reintroduced and is applied for the first time to high frame-rate room-temperature data collection.

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