Cargando…
Changes in the Expression of the Toll-Like Receptor System in the Aging Rat Kidneys
BACKGROUND: The mechanisms of kidney aging are not yet clear. Studies have shown that immunological inflammation is related to kidney aging. Toll-like receptors (TLRs) are one of the receptor types of the body's innate immune system. The function of the TLR system and the mechanisms by which it...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014502/ https://www.ncbi.nlm.nih.gov/pubmed/24810370 http://dx.doi.org/10.1371/journal.pone.0096351 |
_version_ | 1782315186487558144 |
---|---|
author | Xi, Yue Shao, Feng Bai, Xue-Yuan Cai, Guangyan Lv, Yang Chen, Xiangmei |
author_facet | Xi, Yue Shao, Feng Bai, Xue-Yuan Cai, Guangyan Lv, Yang Chen, Xiangmei |
author_sort | Xi, Yue |
collection | PubMed |
description | BACKGROUND: The mechanisms of kidney aging are not yet clear. Studies have shown that immunological inflammation is related to kidney aging. Toll-like receptors (TLRs) are one of the receptor types of the body's innate immune system. The function of the TLR system and the mechanisms by which it functions in renal aging remain unclear. In the present study, we, for the first time, systematically investigated the role of the TLR system and the inflammation responses activated by TLRs during kidney aging. METHODS: We used western blot and immunohistochemistry to systematically analyze the changes in the expression and activation of the endogenous TLR ligands HSP70 and HMGB1, the TLRs (TLR1–TLR11), their downstream signaling pathway molecules MyD88 and Phospho-IRF-3, and the NF-κB signaling pathway molecules Phospho-IKKβ, Phospho-IκBα (NF-κB inhibition factor α), NF-κBp65, and Phospho-NF-κBp65 (activated NF-κB p65) in the kidneys of 3 months old (youth group), 12 months old (middle age group), and 24 months old (elderly group) rats. We used RT-qPCR to detect the mRNA expression changes of the proinflammatory cytokines CCL3, CCL4, CCL5, CD80, TNF-α, and IL-12b in the rat renal tissues of the various age groups. RESULTS: We found that during kidney aging, the HSP70 and HMGB1 expression levels were significantly increased, and the expression levels of TLR1, 2, 3, 4, 5, and 11 and their downstream signaling pathway molecules MyD88 and Phospho-IRF-3 were markedly elevated. Further studies have shown that in the aging kidneys, the expression levels of the NF-κB signaling pathway molecules Phospho-IKKβ, Phospho-IκBα, NF-κBp65, and Phospho-NF-κBp65 were obviously increased, and those of the proinflammatory cytokines CCL3, CCL4, CCL5, CD80, TNF-α, and IL-12b were significantly upregulated. CONCLUSIONS: These results showed that the TLR system might play an important role during the kidney aging process maybe by activating the NF-κB signaling pathway and promoting the high expression of inflammation factors. |
format | Online Article Text |
id | pubmed-4014502 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40145022014-05-14 Changes in the Expression of the Toll-Like Receptor System in the Aging Rat Kidneys Xi, Yue Shao, Feng Bai, Xue-Yuan Cai, Guangyan Lv, Yang Chen, Xiangmei PLoS One Research Article BACKGROUND: The mechanisms of kidney aging are not yet clear. Studies have shown that immunological inflammation is related to kidney aging. Toll-like receptors (TLRs) are one of the receptor types of the body's innate immune system. The function of the TLR system and the mechanisms by which it functions in renal aging remain unclear. In the present study, we, for the first time, systematically investigated the role of the TLR system and the inflammation responses activated by TLRs during kidney aging. METHODS: We used western blot and immunohistochemistry to systematically analyze the changes in the expression and activation of the endogenous TLR ligands HSP70 and HMGB1, the TLRs (TLR1–TLR11), their downstream signaling pathway molecules MyD88 and Phospho-IRF-3, and the NF-κB signaling pathway molecules Phospho-IKKβ, Phospho-IκBα (NF-κB inhibition factor α), NF-κBp65, and Phospho-NF-κBp65 (activated NF-κB p65) in the kidneys of 3 months old (youth group), 12 months old (middle age group), and 24 months old (elderly group) rats. We used RT-qPCR to detect the mRNA expression changes of the proinflammatory cytokines CCL3, CCL4, CCL5, CD80, TNF-α, and IL-12b in the rat renal tissues of the various age groups. RESULTS: We found that during kidney aging, the HSP70 and HMGB1 expression levels were significantly increased, and the expression levels of TLR1, 2, 3, 4, 5, and 11 and their downstream signaling pathway molecules MyD88 and Phospho-IRF-3 were markedly elevated. Further studies have shown that in the aging kidneys, the expression levels of the NF-κB signaling pathway molecules Phospho-IKKβ, Phospho-IκBα, NF-κBp65, and Phospho-NF-κBp65 were obviously increased, and those of the proinflammatory cytokines CCL3, CCL4, CCL5, CD80, TNF-α, and IL-12b were significantly upregulated. CONCLUSIONS: These results showed that the TLR system might play an important role during the kidney aging process maybe by activating the NF-κB signaling pathway and promoting the high expression of inflammation factors. Public Library of Science 2014-05-08 /pmc/articles/PMC4014502/ /pubmed/24810370 http://dx.doi.org/10.1371/journal.pone.0096351 Text en © 2014 Xi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Xi, Yue Shao, Feng Bai, Xue-Yuan Cai, Guangyan Lv, Yang Chen, Xiangmei Changes in the Expression of the Toll-Like Receptor System in the Aging Rat Kidneys |
title | Changes in the Expression of the Toll-Like Receptor System in the Aging Rat Kidneys |
title_full | Changes in the Expression of the Toll-Like Receptor System in the Aging Rat Kidneys |
title_fullStr | Changes in the Expression of the Toll-Like Receptor System in the Aging Rat Kidneys |
title_full_unstemmed | Changes in the Expression of the Toll-Like Receptor System in the Aging Rat Kidneys |
title_short | Changes in the Expression of the Toll-Like Receptor System in the Aging Rat Kidneys |
title_sort | changes in the expression of the toll-like receptor system in the aging rat kidneys |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014502/ https://www.ncbi.nlm.nih.gov/pubmed/24810370 http://dx.doi.org/10.1371/journal.pone.0096351 |
work_keys_str_mv | AT xiyue changesintheexpressionofthetolllikereceptorsystemintheagingratkidneys AT shaofeng changesintheexpressionofthetolllikereceptorsystemintheagingratkidneys AT baixueyuan changesintheexpressionofthetolllikereceptorsystemintheagingratkidneys AT caiguangyan changesintheexpressionofthetolllikereceptorsystemintheagingratkidneys AT lvyang changesintheexpressionofthetolllikereceptorsystemintheagingratkidneys AT chenxiangmei changesintheexpressionofthetolllikereceptorsystemintheagingratkidneys |