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Comprehensive Evaluation and Optimization of Amplicon Library Preparation Methods for High-Throughput Antibody Sequencing

High-throughput sequencing (HTS) of antibody repertoire libraries has become a powerful tool in the field of systems immunology. However, numerous sources of bias in HTS workflows may affect the obtained antibody repertoire data. A crucial step in antibody library preparation is the addition of shor...

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Detalles Bibliográficos
Autores principales:	Menzel, Ulrike, Greiff, Victor, Khan, Tarik A., Haessler, Ulrike, Hellmann, Ina, Friedensohn, Simon, Cook, Skylar C., Pogson, Mark, Reddy, Sai T.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014543/ https://www.ncbi.nlm.nih.gov/pubmed/24809667 http://dx.doi.org/10.1371/journal.pone.0096727

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