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Proteomic Analysis of Mesenchymal Stem Cells from Normal and Deep Carious Dental Pulp

Dental pulp stem cells (DPSCs), precursor cells of odontoblasts, are ideal seed cells for tooth tissue engineering and regeneration. Our previous study has demonstrated that stem cells exist in dental pulp with deep caries and are called carious dental pulp stem cells (CDPSCs). The results indicated...

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Autores principales: Ma, Dandan, Cui, Li, Gao, Jie, Yan, Wenjuan, Liu, Ying, Xu, Shuaimei, Wu, Buling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014579/
https://www.ncbi.nlm.nih.gov/pubmed/24809979
http://dx.doi.org/10.1371/journal.pone.0097026
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author Ma, Dandan
Cui, Li
Gao, Jie
Yan, Wenjuan
Liu, Ying
Xu, Shuaimei
Wu, Buling
author_facet Ma, Dandan
Cui, Li
Gao, Jie
Yan, Wenjuan
Liu, Ying
Xu, Shuaimei
Wu, Buling
author_sort Ma, Dandan
collection PubMed
description Dental pulp stem cells (DPSCs), precursor cells of odontoblasts, are ideal seed cells for tooth tissue engineering and regeneration. Our previous study has demonstrated that stem cells exist in dental pulp with deep caries and are called carious dental pulp stem cells (CDPSCs). The results indicated that CDPSCs had a higher proliferative and stronger osteogenic differentiation potential than DPSCs. However, the molecular mechanisms responsible for the biological differences between DPSCs and CDPSCs are poorly understood. The aim of this study was to define the molecular features of DPSCs and CDPSCs by comparing the proteomic profiles using two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Our results revealed that there were 18 protein spots differentially expressed between DPSCs and CDPSCs in a narrow pH range of 4 to 7. These differently expressed proteins are mostly involved in the regulation of cell proliferation, differentiation, cell cytoskeleton and motility. In addition, our results suggested that CDPSCs had a higher expression of antioxidative proteins that might protect CDPSCs from oxidative stress. This study explores some potential proteins responsible for the biological differences between DPSCs and CDPSCs and expands our understanding on the molecular mechanisms of mineralization of DPSCs in the formation of the dentin-pulp complex.
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spelling pubmed-40145792014-05-14 Proteomic Analysis of Mesenchymal Stem Cells from Normal and Deep Carious Dental Pulp Ma, Dandan Cui, Li Gao, Jie Yan, Wenjuan Liu, Ying Xu, Shuaimei Wu, Buling PLoS One Research Article Dental pulp stem cells (DPSCs), precursor cells of odontoblasts, are ideal seed cells for tooth tissue engineering and regeneration. Our previous study has demonstrated that stem cells exist in dental pulp with deep caries and are called carious dental pulp stem cells (CDPSCs). The results indicated that CDPSCs had a higher proliferative and stronger osteogenic differentiation potential than DPSCs. However, the molecular mechanisms responsible for the biological differences between DPSCs and CDPSCs are poorly understood. The aim of this study was to define the molecular features of DPSCs and CDPSCs by comparing the proteomic profiles using two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Our results revealed that there were 18 protein spots differentially expressed between DPSCs and CDPSCs in a narrow pH range of 4 to 7. These differently expressed proteins are mostly involved in the regulation of cell proliferation, differentiation, cell cytoskeleton and motility. In addition, our results suggested that CDPSCs had a higher expression of antioxidative proteins that might protect CDPSCs from oxidative stress. This study explores some potential proteins responsible for the biological differences between DPSCs and CDPSCs and expands our understanding on the molecular mechanisms of mineralization of DPSCs in the formation of the dentin-pulp complex. Public Library of Science 2014-05-08 /pmc/articles/PMC4014579/ /pubmed/24809979 http://dx.doi.org/10.1371/journal.pone.0097026 Text en © 2014 Ma et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ma, Dandan
Cui, Li
Gao, Jie
Yan, Wenjuan
Liu, Ying
Xu, Shuaimei
Wu, Buling
Proteomic Analysis of Mesenchymal Stem Cells from Normal and Deep Carious Dental Pulp
title Proteomic Analysis of Mesenchymal Stem Cells from Normal and Deep Carious Dental Pulp
title_full Proteomic Analysis of Mesenchymal Stem Cells from Normal and Deep Carious Dental Pulp
title_fullStr Proteomic Analysis of Mesenchymal Stem Cells from Normal and Deep Carious Dental Pulp
title_full_unstemmed Proteomic Analysis of Mesenchymal Stem Cells from Normal and Deep Carious Dental Pulp
title_short Proteomic Analysis of Mesenchymal Stem Cells from Normal and Deep Carious Dental Pulp
title_sort proteomic analysis of mesenchymal stem cells from normal and deep carious dental pulp
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014579/
https://www.ncbi.nlm.nih.gov/pubmed/24809979
http://dx.doi.org/10.1371/journal.pone.0097026
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