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Epigenetic Regulation of Sox30 Is Associated with Testis Development in Mice
DNA methylation is involved in tissue-specific and developmentally regulated gene expression. Here, we screened a novel methylation gene Sox30, whose methylation might contribute to its regulation and testis development in mice. Sox30 is a member of Sox transcription factors, and is considered to be...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014610/ https://www.ncbi.nlm.nih.gov/pubmed/24810894 http://dx.doi.org/10.1371/journal.pone.0097203 |
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author | Han, Fei Dong, Yan Liu, Wenbin Ma, Xuexiang Shi, Ronghui Chen, Hongqiang Cui, Zhihong Ao, Lin Zhang, Huidong Cao, Jia Liu, Jinyi |
author_facet | Han, Fei Dong, Yan Liu, Wenbin Ma, Xuexiang Shi, Ronghui Chen, Hongqiang Cui, Zhihong Ao, Lin Zhang, Huidong Cao, Jia Liu, Jinyi |
author_sort | Han, Fei |
collection | PubMed |
description | DNA methylation is involved in tissue-specific and developmentally regulated gene expression. Here, we screened a novel methylation gene Sox30, whose methylation might contribute to its regulation and testis development in mice. Sox30 is a member of Sox transcription factors, and is considered to be involved in spermatogonial differentiation and spermatogenesis. However, the precise function and regulatory expression pattern remain unclear. In the present study, we found that Sox30 is highly expressed in adult testes but not in ovaries. Sox30 expression begins in early development, and in the testes, it is specifically increased coincidentally with development until adulthood. Moreover, Sox30 is expressed not only in testis germ cells, but also in sertoli cells. Sox30 is hypo-methylated in testis, epididymis and lung of adult mice, in which Sox30 is expressed. By contrast, Sox30 is hypermethylated in ovary, heart, brain, liver, kidney, spleen, pancreas, muscle, intestine, pituitary gland, blood and hippocampus of adult mice, in which the Sox30 is absent. Importantly, decreased methylation at CpG islands of Sox30 is observed in mouse developmental testes after birth, which is associated with enhanced Sox30 expression. However, the hypermethylated status of Sox30 is maintained in ovaries that does not express Sox30 during this period. Further, following demethylation treatment using 5-aza-dC, Sox30 expression is restored in GC2, TM3 and TM4 cell lines. This observation convincingly confirms that methylation really contributes to Sox30 silencing. In summary, we show that Sox30 expression is under the control of DNA methylation status, and this expression pattern is associated with testis development in mice. |
format | Online Article Text |
id | pubmed-4014610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40146102014-05-14 Epigenetic Regulation of Sox30 Is Associated with Testis Development in Mice Han, Fei Dong, Yan Liu, Wenbin Ma, Xuexiang Shi, Ronghui Chen, Hongqiang Cui, Zhihong Ao, Lin Zhang, Huidong Cao, Jia Liu, Jinyi PLoS One Research Article DNA methylation is involved in tissue-specific and developmentally regulated gene expression. Here, we screened a novel methylation gene Sox30, whose methylation might contribute to its regulation and testis development in mice. Sox30 is a member of Sox transcription factors, and is considered to be involved in spermatogonial differentiation and spermatogenesis. However, the precise function and regulatory expression pattern remain unclear. In the present study, we found that Sox30 is highly expressed in adult testes but not in ovaries. Sox30 expression begins in early development, and in the testes, it is specifically increased coincidentally with development until adulthood. Moreover, Sox30 is expressed not only in testis germ cells, but also in sertoli cells. Sox30 is hypo-methylated in testis, epididymis and lung of adult mice, in which Sox30 is expressed. By contrast, Sox30 is hypermethylated in ovary, heart, brain, liver, kidney, spleen, pancreas, muscle, intestine, pituitary gland, blood and hippocampus of adult mice, in which the Sox30 is absent. Importantly, decreased methylation at CpG islands of Sox30 is observed in mouse developmental testes after birth, which is associated with enhanced Sox30 expression. However, the hypermethylated status of Sox30 is maintained in ovaries that does not express Sox30 during this period. Further, following demethylation treatment using 5-aza-dC, Sox30 expression is restored in GC2, TM3 and TM4 cell lines. This observation convincingly confirms that methylation really contributes to Sox30 silencing. In summary, we show that Sox30 expression is under the control of DNA methylation status, and this expression pattern is associated with testis development in mice. Public Library of Science 2014-05-08 /pmc/articles/PMC4014610/ /pubmed/24810894 http://dx.doi.org/10.1371/journal.pone.0097203 Text en © 2014 Han et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Han, Fei Dong, Yan Liu, Wenbin Ma, Xuexiang Shi, Ronghui Chen, Hongqiang Cui, Zhihong Ao, Lin Zhang, Huidong Cao, Jia Liu, Jinyi Epigenetic Regulation of Sox30 Is Associated with Testis Development in Mice |
title | Epigenetic Regulation of Sox30 Is Associated with Testis Development in Mice |
title_full | Epigenetic Regulation of Sox30 Is Associated with Testis Development in Mice |
title_fullStr | Epigenetic Regulation of Sox30 Is Associated with Testis Development in Mice |
title_full_unstemmed | Epigenetic Regulation of Sox30 Is Associated with Testis Development in Mice |
title_short | Epigenetic Regulation of Sox30 Is Associated with Testis Development in Mice |
title_sort | epigenetic regulation of sox30 is associated with testis development in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4014610/ https://www.ncbi.nlm.nih.gov/pubmed/24810894 http://dx.doi.org/10.1371/journal.pone.0097203 |
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