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Autonomic Nerve Regulation of Colonic Peristalsis in Guinea Pigs

BACKGROUND/AIMS: Colonic peristalsis is mainly regulated via intrinsic neurons in guinea pigs. However, autonomic regulation of colonic motility is poorly understood. We explored a guinea pig model for the study of extrinsic nerve effects on the distal colon. METHODS: Guinea pigs were sacrificed, th...

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Autores principales: Gribovskaja-Rupp, Irena, Babygirija, Reji, Takahashi, Toku, Ludwig, Kirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Neurogastroenterology and Motility 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015210/
https://www.ncbi.nlm.nih.gov/pubmed/24847719
http://dx.doi.org/10.5056/jnm.2014.20.2.185
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author Gribovskaja-Rupp, Irena
Babygirija, Reji
Takahashi, Toku
Ludwig, Kirk
author_facet Gribovskaja-Rupp, Irena
Babygirija, Reji
Takahashi, Toku
Ludwig, Kirk
author_sort Gribovskaja-Rupp, Irena
collection PubMed
description BACKGROUND/AIMS: Colonic peristalsis is mainly regulated via intrinsic neurons in guinea pigs. However, autonomic regulation of colonic motility is poorly understood. We explored a guinea pig model for the study of extrinsic nerve effects on the distal colon. METHODS: Guinea pigs were sacrificed, their distal colons isolated, preserving pelvic nerves (PN) and inferior mesenteric ganglia (IMG), and placed in a tissue bath. Fecal pellet propagation was conducted during PN and IMG stimulation at 10 Hz, 0.5 ms and 5 V. Distal colon was connected to a closed circuit system, and colonic motor responses were measured during PN and IMG stimulation. RESULTS: PN stimulation increased pellet velocity to 24.6 ± 0.7 mm/sec (n = 20), while IMG stimulation decreased it to 2.0 ± 0.2 mm/sec (n = 12), compared to controls (13.0 ± 0.7 mm/sec, P < 0.01). In closed circuit experiments, PN stimulation increased the intraluminal pressure, which was abolished by atropine (10(−6) M) and hexamethonium (10(−4) M). PN stimulation reduced the incidence of non-coordinated contractions induced by NG-nitro-L-arginine methyl ester (L-NAME; 10(−4) M). IMG stimulation attenuated intraluminal pressure increase, which was partially reversed by alpha-2 adrenoceptor antagonist (yohimbine; 10(−6) M). CONCLUSIONS: PN and IMG input determine speed of pellet progression and peristaltic reflex of the guinea pig distal colon. The stimulatory effects of PN involve nicotinic, muscarinic and nitrergic pathways. The inhibitory effects of IMG stimulation involve alpha-2 adrenoceptors.
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spelling pubmed-40152102014-05-12 Autonomic Nerve Regulation of Colonic Peristalsis in Guinea Pigs Gribovskaja-Rupp, Irena Babygirija, Reji Takahashi, Toku Ludwig, Kirk J Neurogastroenterol Motil Original Article BACKGROUND/AIMS: Colonic peristalsis is mainly regulated via intrinsic neurons in guinea pigs. However, autonomic regulation of colonic motility is poorly understood. We explored a guinea pig model for the study of extrinsic nerve effects on the distal colon. METHODS: Guinea pigs were sacrificed, their distal colons isolated, preserving pelvic nerves (PN) and inferior mesenteric ganglia (IMG), and placed in a tissue bath. Fecal pellet propagation was conducted during PN and IMG stimulation at 10 Hz, 0.5 ms and 5 V. Distal colon was connected to a closed circuit system, and colonic motor responses were measured during PN and IMG stimulation. RESULTS: PN stimulation increased pellet velocity to 24.6 ± 0.7 mm/sec (n = 20), while IMG stimulation decreased it to 2.0 ± 0.2 mm/sec (n = 12), compared to controls (13.0 ± 0.7 mm/sec, P < 0.01). In closed circuit experiments, PN stimulation increased the intraluminal pressure, which was abolished by atropine (10(−6) M) and hexamethonium (10(−4) M). PN stimulation reduced the incidence of non-coordinated contractions induced by NG-nitro-L-arginine methyl ester (L-NAME; 10(−4) M). IMG stimulation attenuated intraluminal pressure increase, which was partially reversed by alpha-2 adrenoceptor antagonist (yohimbine; 10(−6) M). CONCLUSIONS: PN and IMG input determine speed of pellet progression and peristaltic reflex of the guinea pig distal colon. The stimulatory effects of PN involve nicotinic, muscarinic and nitrergic pathways. The inhibitory effects of IMG stimulation involve alpha-2 adrenoceptors. Korean Society of Neurogastroenterology and Motility 2014-04 /pmc/articles/PMC4015210/ /pubmed/24847719 http://dx.doi.org/10.5056/jnm.2014.20.2.185 Text en © 2014 The Korean Society of Neurogastroenterology and Motility This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Gribovskaja-Rupp, Irena
Babygirija, Reji
Takahashi, Toku
Ludwig, Kirk
Autonomic Nerve Regulation of Colonic Peristalsis in Guinea Pigs
title Autonomic Nerve Regulation of Colonic Peristalsis in Guinea Pigs
title_full Autonomic Nerve Regulation of Colonic Peristalsis in Guinea Pigs
title_fullStr Autonomic Nerve Regulation of Colonic Peristalsis in Guinea Pigs
title_full_unstemmed Autonomic Nerve Regulation of Colonic Peristalsis in Guinea Pigs
title_short Autonomic Nerve Regulation of Colonic Peristalsis in Guinea Pigs
title_sort autonomic nerve regulation of colonic peristalsis in guinea pigs
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015210/
https://www.ncbi.nlm.nih.gov/pubmed/24847719
http://dx.doi.org/10.5056/jnm.2014.20.2.185
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