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Specificity of the Receptor for the Major Sex Pheromone Component in Heliothis virescens

In a previous study, the Drosophila melanogaster OR67d(GAL4);UAS system was used to functionally characterize the receptor for the major component of the sex pheromone in the tobacco budworm, Heliothis virescens Fabricius (Lepidoptera: Noctuidae), HvOR13. Electrophysiological and behavioral assays s...

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Autores principales: Vásquez, Gissella M., Syed, Zainulabeuddin, Estes, Patricia A., Leal, Walter S., Gould, Fred
Formato: Online Artículo Texto
Lenguaje:English
Publicado: University of Wisconsin Library 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015405/
https://www.ncbi.nlm.nih.gov/pubmed/24773407
http://dx.doi.org/10.1673/031.013.16001
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author Vásquez, Gissella M.
Syed, Zainulabeuddin
Estes, Patricia A.
Leal, Walter S.
Gould, Fred
author_facet Vásquez, Gissella M.
Syed, Zainulabeuddin
Estes, Patricia A.
Leal, Walter S.
Gould, Fred
author_sort Vásquez, Gissella M.
collection PubMed
description In a previous study, the Drosophila melanogaster OR67d(GAL4);UAS system was used to functionally characterize the receptor for the major component of the sex pheromone in the tobacco budworm, Heliothis virescens Fabricius (Lepidoptera: Noctuidae), HvOR13. Electrophysiological and behavioral assays showed that transgenic flies expressing HvOR13 responded to (Z)-11-hexadecenal (Z11-16:Ald). However, tests were not performed to determine whether these flies would also respond to secondary components of the H. virescens sex pheromone. Thus, in this study the response spectrum of HvOR13 expressed in this system was examined by performing single cell recordings from odor receptor neuron in trichoid T1 sensilla on antennae of two Or67d(GAL4 [1]); UAS-HvOR13 lines stimulated with Z11-16:Ald and six H. virescens secondary pheromone components. Fly courtship assays were also performed to examine the behavioral response of the Or67d(GAL4[1]); UAS-HvOR13 flies to Z11-16:Ald and the secondary component Z9-14:Ald. Our combined electrophysiological and behavioral studies indicated high specificity and sensitivity of HvOR13 to Z11-16:Ald. Interestingly, a mutation leading to truncation in the HvOR13 C-terminal region affected but did not abolish pheromone receptor response to Z11-16:Ald. The findings are assessed in relationship to other HvOR13 heterologous expression studies, and the role of the C-terminal domain in receptor function is discussed. A third line expressing HvOR15 was also tested but did not respond to any of the seven pheromone components.
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spelling pubmed-40154052014-05-14 Specificity of the Receptor for the Major Sex Pheromone Component in Heliothis virescens Vásquez, Gissella M. Syed, Zainulabeuddin Estes, Patricia A. Leal, Walter S. Gould, Fred J Insect Sci Article In a previous study, the Drosophila melanogaster OR67d(GAL4);UAS system was used to functionally characterize the receptor for the major component of the sex pheromone in the tobacco budworm, Heliothis virescens Fabricius (Lepidoptera: Noctuidae), HvOR13. Electrophysiological and behavioral assays showed that transgenic flies expressing HvOR13 responded to (Z)-11-hexadecenal (Z11-16:Ald). However, tests were not performed to determine whether these flies would also respond to secondary components of the H. virescens sex pheromone. Thus, in this study the response spectrum of HvOR13 expressed in this system was examined by performing single cell recordings from odor receptor neuron in trichoid T1 sensilla on antennae of two Or67d(GAL4 [1]); UAS-HvOR13 lines stimulated with Z11-16:Ald and six H. virescens secondary pheromone components. Fly courtship assays were also performed to examine the behavioral response of the Or67d(GAL4[1]); UAS-HvOR13 flies to Z11-16:Ald and the secondary component Z9-14:Ald. Our combined electrophysiological and behavioral studies indicated high specificity and sensitivity of HvOR13 to Z11-16:Ald. Interestingly, a mutation leading to truncation in the HvOR13 C-terminal region affected but did not abolish pheromone receptor response to Z11-16:Ald. The findings are assessed in relationship to other HvOR13 heterologous expression studies, and the role of the C-terminal domain in receptor function is discussed. A third line expressing HvOR15 was also tested but did not respond to any of the seven pheromone components. University of Wisconsin Library 2013-12-25 /pmc/articles/PMC4015405/ /pubmed/24773407 http://dx.doi.org/10.1673/031.013.16001 Text en © 2013 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Vásquez, Gissella M.
Syed, Zainulabeuddin
Estes, Patricia A.
Leal, Walter S.
Gould, Fred
Specificity of the Receptor for the Major Sex Pheromone Component in Heliothis virescens
title Specificity of the Receptor for the Major Sex Pheromone Component in Heliothis virescens
title_full Specificity of the Receptor for the Major Sex Pheromone Component in Heliothis virescens
title_fullStr Specificity of the Receptor for the Major Sex Pheromone Component in Heliothis virescens
title_full_unstemmed Specificity of the Receptor for the Major Sex Pheromone Component in Heliothis virescens
title_short Specificity of the Receptor for the Major Sex Pheromone Component in Heliothis virescens
title_sort specificity of the receptor for the major sex pheromone component in heliothis virescens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015405/
https://www.ncbi.nlm.nih.gov/pubmed/24773407
http://dx.doi.org/10.1673/031.013.16001
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