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Gold mining areas in Suriname: reservoirs of malaria resistance?
BACKGROUND: At present, malaria cases in Suriname occur predominantly in migrants and people living and/or working in areas with gold mining operations. A molecular survey was performed in Plasmodium falciparum isolates originating from persons from gold mining areas to assess the extent and role of...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015718/ https://www.ncbi.nlm.nih.gov/pubmed/24833911 http://dx.doi.org/10.2147/IDR.S60906 |
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author | Adhin, Malti R Labadie-Bracho, Mergiory Vreden, Stephen |
author_facet | Adhin, Malti R Labadie-Bracho, Mergiory Vreden, Stephen |
author_sort | Adhin, Malti R |
collection | PubMed |
description | BACKGROUND: At present, malaria cases in Suriname occur predominantly in migrants and people living and/or working in areas with gold mining operations. A molecular survey was performed in Plasmodium falciparum isolates originating from persons from gold mining areas to assess the extent and role of mining areas as reservoirs of malaria resistance in Suriname. METHODS: The status of 14 putative resistance-associated single nucleotide polymorphisms in the pfdhfr, pfcrt, pfmdr1, and pfATP6 genes was assessed for 28 samples from gold miners diagnosed with P. falciparum malaria using polymerase chain reaction amplification and restriction fragment length polymorphism analysis, and the results were compared with earlier data from nonmining villagers. RESULTS: Isolates from miners showed a high degree of homogeneity, with a fixed pfdhfr Ile51/Asn108, pfmdr1 Phe184/Asp1042/Tyr1246, and pfcrt Thr76 mutant genotype, while an exclusively wild-type genotype was observed for pfmdr1 Asn86 and pfdhfr Ala16, Cys59, and Ile164, and for the pfATP6 positions Leu263/Ala623/Ser769. Small variations were observed for pfmdr1 S1034C. No statistically significant difference could be detected in allele frequencies between mining and nonmining villagers. CONCLUSION: Despite the increased risk of malaria infection in individuals working/living in gold mining areas, we did not detect an increase in mutation frequency at the 14 analyzed single nucleotide polymorphisms. Therefore, mining areas in Suriname cannot yet be considered as reservoirs for malaria resistance. |
format | Online Article Text |
id | pubmed-4015718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-40157182014-05-15 Gold mining areas in Suriname: reservoirs of malaria resistance? Adhin, Malti R Labadie-Bracho, Mergiory Vreden, Stephen Infect Drug Resist Original Research BACKGROUND: At present, malaria cases in Suriname occur predominantly in migrants and people living and/or working in areas with gold mining operations. A molecular survey was performed in Plasmodium falciparum isolates originating from persons from gold mining areas to assess the extent and role of mining areas as reservoirs of malaria resistance in Suriname. METHODS: The status of 14 putative resistance-associated single nucleotide polymorphisms in the pfdhfr, pfcrt, pfmdr1, and pfATP6 genes was assessed for 28 samples from gold miners diagnosed with P. falciparum malaria using polymerase chain reaction amplification and restriction fragment length polymorphism analysis, and the results were compared with earlier data from nonmining villagers. RESULTS: Isolates from miners showed a high degree of homogeneity, with a fixed pfdhfr Ile51/Asn108, pfmdr1 Phe184/Asp1042/Tyr1246, and pfcrt Thr76 mutant genotype, while an exclusively wild-type genotype was observed for pfmdr1 Asn86 and pfdhfr Ala16, Cys59, and Ile164, and for the pfATP6 positions Leu263/Ala623/Ser769. Small variations were observed for pfmdr1 S1034C. No statistically significant difference could be detected in allele frequencies between mining and nonmining villagers. CONCLUSION: Despite the increased risk of malaria infection in individuals working/living in gold mining areas, we did not detect an increase in mutation frequency at the 14 analyzed single nucleotide polymorphisms. Therefore, mining areas in Suriname cannot yet be considered as reservoirs for malaria resistance. Dove Medical Press 2014-05-05 /pmc/articles/PMC4015718/ /pubmed/24833911 http://dx.doi.org/10.2147/IDR.S60906 Text en © 2014 Adhin et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Adhin, Malti R Labadie-Bracho, Mergiory Vreden, Stephen Gold mining areas in Suriname: reservoirs of malaria resistance? |
title | Gold mining areas in Suriname: reservoirs of malaria resistance? |
title_full | Gold mining areas in Suriname: reservoirs of malaria resistance? |
title_fullStr | Gold mining areas in Suriname: reservoirs of malaria resistance? |
title_full_unstemmed | Gold mining areas in Suriname: reservoirs of malaria resistance? |
title_short | Gold mining areas in Suriname: reservoirs of malaria resistance? |
title_sort | gold mining areas in suriname: reservoirs of malaria resistance? |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015718/ https://www.ncbi.nlm.nih.gov/pubmed/24833911 http://dx.doi.org/10.2147/IDR.S60906 |
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