Cargando…
Proteins of novel lactic acid bacteria from Apis mellifera mellifera: an insight into the production of known extra-cellular proteins during microbial stress
BACKGROUND: Lactic acid bacteria (LAB) has been considered a beneficial bacterial group, found as part of the microbiota of diverse hosts, including humans and various animals. However, the mechanisms of how hosts and LAB interact are still poorly understood. Previous work demonstrates that 13 speci...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015849/ https://www.ncbi.nlm.nih.gov/pubmed/24148670 http://dx.doi.org/10.1186/1471-2180-13-235 |
_version_ | 1782315413089026048 |
---|---|
author | Butler, Èile Alsterfjord, Magnus Olofsson, Tobias C Karlsson, Christofer Malmström, Johan Vásquez, Alejandra |
author_facet | Butler, Èile Alsterfjord, Magnus Olofsson, Tobias C Karlsson, Christofer Malmström, Johan Vásquez, Alejandra |
author_sort | Butler, Èile |
collection | PubMed |
description | BACKGROUND: Lactic acid bacteria (LAB) has been considered a beneficial bacterial group, found as part of the microbiota of diverse hosts, including humans and various animals. However, the mechanisms of how hosts and LAB interact are still poorly understood. Previous work demonstrates that 13 species of Lactobacillus and Bifidobacterium from the honey crop in bees function symbiotically with the honeybee. They protect each other, their hosts, and the surrounding environment against severe bee pathogens, bacteria, and yeasts. Therefore, we hypothesized that these LAB under stress, i.e. in their natural niche in the honey crop, are likely to produce bioactive substances with antimicrobial activity. RESULTS: The genomic analysis of the LAB demonstrated varying genome sizes ranging from 1.5 to 2.2 mega-base pairs (Mbps) which points out a clear difference within the protein gene content, as well as specialized functions in the honeybee microbiota and their adaptation to their host. We demonstrate a clear variation between the secreted proteins of the symbiotic LAB when subjected to microbial stressors. We have identified that 10 of the 13 LAB produced extra-cellular proteins of known or unknown function in which some are arranged in interesting putative operons that may be involved in antimicrobial action, host interaction, or biofilm formation. The most common known extra-cellular proteins secreted were enzymes, DNA chaperones, S-layer proteins, bacteriocins, and lysozymes. A new bacteriocin may have been identified in one of the LAB symbionts while many proteins with unknown functions were produced which must be investigated further. CONCLUSIONS: The 13 LAB symbionts likely play different roles in their natural environment defending their niche and their host and participating in the honeybee’s food production. These roles are partly played through producing extracellular proteins on exposure to microbial stressors widely found in natural occurring flowers. Many of these secreted proteins may have a putative antimicrobial function. In the future, understanding these processes in this complicated environment may lead to novel applications of honey crop LAB proteins. |
format | Online Article Text |
id | pubmed-4015849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-40158492014-05-10 Proteins of novel lactic acid bacteria from Apis mellifera mellifera: an insight into the production of known extra-cellular proteins during microbial stress Butler, Èile Alsterfjord, Magnus Olofsson, Tobias C Karlsson, Christofer Malmström, Johan Vásquez, Alejandra BMC Microbiol Research Article BACKGROUND: Lactic acid bacteria (LAB) has been considered a beneficial bacterial group, found as part of the microbiota of diverse hosts, including humans and various animals. However, the mechanisms of how hosts and LAB interact are still poorly understood. Previous work demonstrates that 13 species of Lactobacillus and Bifidobacterium from the honey crop in bees function symbiotically with the honeybee. They protect each other, their hosts, and the surrounding environment against severe bee pathogens, bacteria, and yeasts. Therefore, we hypothesized that these LAB under stress, i.e. in their natural niche in the honey crop, are likely to produce bioactive substances with antimicrobial activity. RESULTS: The genomic analysis of the LAB demonstrated varying genome sizes ranging from 1.5 to 2.2 mega-base pairs (Mbps) which points out a clear difference within the protein gene content, as well as specialized functions in the honeybee microbiota and their adaptation to their host. We demonstrate a clear variation between the secreted proteins of the symbiotic LAB when subjected to microbial stressors. We have identified that 10 of the 13 LAB produced extra-cellular proteins of known or unknown function in which some are arranged in interesting putative operons that may be involved in antimicrobial action, host interaction, or biofilm formation. The most common known extra-cellular proteins secreted were enzymes, DNA chaperones, S-layer proteins, bacteriocins, and lysozymes. A new bacteriocin may have been identified in one of the LAB symbionts while many proteins with unknown functions were produced which must be investigated further. CONCLUSIONS: The 13 LAB symbionts likely play different roles in their natural environment defending their niche and their host and participating in the honeybee’s food production. These roles are partly played through producing extracellular proteins on exposure to microbial stressors widely found in natural occurring flowers. Many of these secreted proteins may have a putative antimicrobial function. In the future, understanding these processes in this complicated environment may lead to novel applications of honey crop LAB proteins. BioMed Central 2013-10-22 /pmc/articles/PMC4015849/ /pubmed/24148670 http://dx.doi.org/10.1186/1471-2180-13-235 Text en Copyright © 2013 Butler et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Butler, Èile Alsterfjord, Magnus Olofsson, Tobias C Karlsson, Christofer Malmström, Johan Vásquez, Alejandra Proteins of novel lactic acid bacteria from Apis mellifera mellifera: an insight into the production of known extra-cellular proteins during microbial stress |
title | Proteins of novel lactic acid bacteria from Apis mellifera mellifera: an insight into the production of known extra-cellular proteins during microbial stress |
title_full | Proteins of novel lactic acid bacteria from Apis mellifera mellifera: an insight into the production of known extra-cellular proteins during microbial stress |
title_fullStr | Proteins of novel lactic acid bacteria from Apis mellifera mellifera: an insight into the production of known extra-cellular proteins during microbial stress |
title_full_unstemmed | Proteins of novel lactic acid bacteria from Apis mellifera mellifera: an insight into the production of known extra-cellular proteins during microbial stress |
title_short | Proteins of novel lactic acid bacteria from Apis mellifera mellifera: an insight into the production of known extra-cellular proteins during microbial stress |
title_sort | proteins of novel lactic acid bacteria from apis mellifera mellifera: an insight into the production of known extra-cellular proteins during microbial stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015849/ https://www.ncbi.nlm.nih.gov/pubmed/24148670 http://dx.doi.org/10.1186/1471-2180-13-235 |
work_keys_str_mv | AT butlereile proteinsofnovellacticacidbacteriafromapismelliferamelliferaaninsightintotheproductionofknownextracellularproteinsduringmicrobialstress AT alsterfjordmagnus proteinsofnovellacticacidbacteriafromapismelliferamelliferaaninsightintotheproductionofknownextracellularproteinsduringmicrobialstress AT olofssontobiasc proteinsofnovellacticacidbacteriafromapismelliferamelliferaaninsightintotheproductionofknownextracellularproteinsduringmicrobialstress AT karlssonchristofer proteinsofnovellacticacidbacteriafromapismelliferamelliferaaninsightintotheproductionofknownextracellularproteinsduringmicrobialstress AT malmstromjohan proteinsofnovellacticacidbacteriafromapismelliferamelliferaaninsightintotheproductionofknownextracellularproteinsduringmicrobialstress AT vasquezalejandra proteinsofnovellacticacidbacteriafromapismelliferamelliferaaninsightintotheproductionofknownextracellularproteinsduringmicrobialstress |