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Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV
Mitochondria are involved in the regulation of cell differentiation processes, but its function changes and molecular mechanisms are not yet clear. In this study, we found that mitochondrial functions changed obviously when K562 cells were induced to megakaryocytic differentiation by phorbol 12-myri...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015910/ https://www.ncbi.nlm.nih.gov/pubmed/24817082 http://dx.doi.org/10.1371/journal.pone.0096246 |
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author | Huang, Rui Zhao, Long Chen, Hui Yin, Rong-Hua Li, Chang-Yan Zhan, Yi-Qun Zhang, Jian-Hong Ge, Chang-hui Yu, Miao Yang, Xiao-Ming |
author_facet | Huang, Rui Zhao, Long Chen, Hui Yin, Rong-Hua Li, Chang-Yan Zhan, Yi-Qun Zhang, Jian-Hong Ge, Chang-hui Yu, Miao Yang, Xiao-Ming |
author_sort | Huang, Rui |
collection | PubMed |
description | Mitochondria are involved in the regulation of cell differentiation processes, but its function changes and molecular mechanisms are not yet clear. In this study, we found that mitochondrial functions changed obviously when K562 cells were induced to megakaryocytic differentiation by phorbol 12-myristate 13-acetate (PMA). During the cell differentiation, the reactive oxygen species (ROS) level was increased, mitochondrial membrane potential declined and respiratory chain complex IV activity was decreased. Treatment with specific inhibitor of mitochondrial respiratory chain complex IV led to a significant inhibition in mitochondrial membrane potential and reduction of PMA-induced cell differentiation. However, treatment with cyclosporine A, a stabilization reagent of mitochondrial membrane potential, did not improve the down-regulation of mitochondrial respiratory chain complex IV induced by PMA. Furthermore, we found that the level of the complex IV core subunit COX3 and mitochondrial transport-related proteins Tim9 and Tim10 were decreased during the differentiation of K562 cells induced by PMA, suggesting an important role of these factors in mitochondrial functional changes. Our results suggest that changes in mitochondrial functions are involved in the PMA-induced K562 cell differentiation process, and the maintenance of the steady-state of mitochondrial functions plays a critical role in the regulation of cell differentiation. |
format | Online Article Text |
id | pubmed-4015910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40159102014-05-14 Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV Huang, Rui Zhao, Long Chen, Hui Yin, Rong-Hua Li, Chang-Yan Zhan, Yi-Qun Zhang, Jian-Hong Ge, Chang-hui Yu, Miao Yang, Xiao-Ming PLoS One Research Article Mitochondria are involved in the regulation of cell differentiation processes, but its function changes and molecular mechanisms are not yet clear. In this study, we found that mitochondrial functions changed obviously when K562 cells were induced to megakaryocytic differentiation by phorbol 12-myristate 13-acetate (PMA). During the cell differentiation, the reactive oxygen species (ROS) level was increased, mitochondrial membrane potential declined and respiratory chain complex IV activity was decreased. Treatment with specific inhibitor of mitochondrial respiratory chain complex IV led to a significant inhibition in mitochondrial membrane potential and reduction of PMA-induced cell differentiation. However, treatment with cyclosporine A, a stabilization reagent of mitochondrial membrane potential, did not improve the down-regulation of mitochondrial respiratory chain complex IV induced by PMA. Furthermore, we found that the level of the complex IV core subunit COX3 and mitochondrial transport-related proteins Tim9 and Tim10 were decreased during the differentiation of K562 cells induced by PMA, suggesting an important role of these factors in mitochondrial functional changes. Our results suggest that changes in mitochondrial functions are involved in the PMA-induced K562 cell differentiation process, and the maintenance of the steady-state of mitochondrial functions plays a critical role in the regulation of cell differentiation. Public Library of Science 2014-05-09 /pmc/articles/PMC4015910/ /pubmed/24817082 http://dx.doi.org/10.1371/journal.pone.0096246 Text en © 2014 Huang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Huang, Rui Zhao, Long Chen, Hui Yin, Rong-Hua Li, Chang-Yan Zhan, Yi-Qun Zhang, Jian-Hong Ge, Chang-hui Yu, Miao Yang, Xiao-Ming Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV |
title | Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV |
title_full | Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV |
title_fullStr | Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV |
title_full_unstemmed | Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV |
title_short | Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV |
title_sort | megakaryocytic differentiation of k562 cells induced by pma reduced the activity of respiratory chain complex iv |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015910/ https://www.ncbi.nlm.nih.gov/pubmed/24817082 http://dx.doi.org/10.1371/journal.pone.0096246 |
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