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Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges
BACKGROUND: Resilience or the ability of our body to cope with daily-life challenges has been proposed as a new definition of health, with restoration of homeostasis as target resultant of various physiological stress responses. Challenge models may thus be a sensitive measure to study the body’s he...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015956/ https://www.ncbi.nlm.nih.gov/pubmed/24160467 http://dx.doi.org/10.1186/1755-8794-6-44 |
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author | Wopereis, Suzan Wolvers, Danielle van Erk, Marjan Gribnau, Michiel Kremer, Bas van Dorsten, Ferdi A Boelsma, Esther Garczarek, Ursula Cnubben, Nicole Frenken, Leon van der Logt, Paul Hendriks, Henk FJ Albers, Ruud van Duynhoven, John van Ommen, Ben Jacobs, Doris M |
author_facet | Wopereis, Suzan Wolvers, Danielle van Erk, Marjan Gribnau, Michiel Kremer, Bas van Dorsten, Ferdi A Boelsma, Esther Garczarek, Ursula Cnubben, Nicole Frenken, Leon van der Logt, Paul Hendriks, Henk FJ Albers, Ruud van Duynhoven, John van Ommen, Ben Jacobs, Doris M |
author_sort | Wopereis, Suzan |
collection | PubMed |
description | BACKGROUND: Resilience or the ability of our body to cope with daily-life challenges has been proposed as a new definition of health, with restoration of homeostasis as target resultant of various physiological stress responses. Challenge models may thus be a sensitive measure to study the body’s health. The objective of this study was to select a dietary challenge model for the assessment of inflammatory resilience. Meals are a challenge to metabolic homeostasis and are suggested to affect inflammatory pathways, yet data in literature are limited and inconsistent. METHOD: The kinetic responses of three different dietary challenges and a water control challenge were assessed on various metabolic and inflammatory markers in 14 healthy males and females using a full cross-over study design. The dietary challenges included glucose (75 g glucose in 300 ml water), lipids (200 ml whipping cream) and a mix of glucose and lipids (same amounts as above), respectively. Blood samples were collected at baseline and at 0.5, 1, 2, 4, 6, 8 and 10 h after consumption of the treatment products. Inflammation (IFNγ, IL-1β, IL-6, IL-8, IL-10, IL-12p70, TNF-α CRP, ICAM-1, VCAM-1, SAA, E-selectin, P-selectin, thrombomodulin, leukocytes, neutrophils, lymphocytes) and clinical (e.g. glucose, insulin, triglycerides) markers as well as gene expression in blood cells and plasma oxylipin profiles were measured. RESULTS: All three dietary challenges induced changes related to metabolic control such as increases in glucose and insulin after the glucose challenge and increases in triglycerides after the lipid challenge. In addition, differences between the challenges were observed for precursor oxylipins and some downstream metabolites including DiHETrE’s and HODE’s. However, none of the dietary challenges induced an acute inflammatory response, except for a modest increase in circulating leukocyte numbers after the glucose and mix challenges. Furthermore, subtle, yet statistically significant increases in vascular inflammatory markers (sICAM-1 and sVCAM-1) were found after the mix challenge, when compared to the water control challenge. CONCLUSIONS: This study shows that dietary glucose and lipid challenges did not induce a strong acute inflammatory response in healthy subjects, as quantified by an accurate and broad panel of parameters. |
format | Online Article Text |
id | pubmed-4015956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-40159562014-05-10 Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges Wopereis, Suzan Wolvers, Danielle van Erk, Marjan Gribnau, Michiel Kremer, Bas van Dorsten, Ferdi A Boelsma, Esther Garczarek, Ursula Cnubben, Nicole Frenken, Leon van der Logt, Paul Hendriks, Henk FJ Albers, Ruud van Duynhoven, John van Ommen, Ben Jacobs, Doris M BMC Med Genomics Research Article BACKGROUND: Resilience or the ability of our body to cope with daily-life challenges has been proposed as a new definition of health, with restoration of homeostasis as target resultant of various physiological stress responses. Challenge models may thus be a sensitive measure to study the body’s health. The objective of this study was to select a dietary challenge model for the assessment of inflammatory resilience. Meals are a challenge to metabolic homeostasis and are suggested to affect inflammatory pathways, yet data in literature are limited and inconsistent. METHOD: The kinetic responses of three different dietary challenges and a water control challenge were assessed on various metabolic and inflammatory markers in 14 healthy males and females using a full cross-over study design. The dietary challenges included glucose (75 g glucose in 300 ml water), lipids (200 ml whipping cream) and a mix of glucose and lipids (same amounts as above), respectively. Blood samples were collected at baseline and at 0.5, 1, 2, 4, 6, 8 and 10 h after consumption of the treatment products. Inflammation (IFNγ, IL-1β, IL-6, IL-8, IL-10, IL-12p70, TNF-α CRP, ICAM-1, VCAM-1, SAA, E-selectin, P-selectin, thrombomodulin, leukocytes, neutrophils, lymphocytes) and clinical (e.g. glucose, insulin, triglycerides) markers as well as gene expression in blood cells and plasma oxylipin profiles were measured. RESULTS: All three dietary challenges induced changes related to metabolic control such as increases in glucose and insulin after the glucose challenge and increases in triglycerides after the lipid challenge. In addition, differences between the challenges were observed for precursor oxylipins and some downstream metabolites including DiHETrE’s and HODE’s. However, none of the dietary challenges induced an acute inflammatory response, except for a modest increase in circulating leukocyte numbers after the glucose and mix challenges. Furthermore, subtle, yet statistically significant increases in vascular inflammatory markers (sICAM-1 and sVCAM-1) were found after the mix challenge, when compared to the water control challenge. CONCLUSIONS: This study shows that dietary glucose and lipid challenges did not induce a strong acute inflammatory response in healthy subjects, as quantified by an accurate and broad panel of parameters. BioMed Central 2013-10-27 /pmc/articles/PMC4015956/ /pubmed/24160467 http://dx.doi.org/10.1186/1755-8794-6-44 Text en Copyright © 2013 Wopereis et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wopereis, Suzan Wolvers, Danielle van Erk, Marjan Gribnau, Michiel Kremer, Bas van Dorsten, Ferdi A Boelsma, Esther Garczarek, Ursula Cnubben, Nicole Frenken, Leon van der Logt, Paul Hendriks, Henk FJ Albers, Ruud van Duynhoven, John van Ommen, Ben Jacobs, Doris M Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges |
title | Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges |
title_full | Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges |
title_fullStr | Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges |
title_full_unstemmed | Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges |
title_short | Assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges |
title_sort | assessment of inflammatory resilience in healthy subjects using dietary lipid and glucose challenges |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015956/ https://www.ncbi.nlm.nih.gov/pubmed/24160467 http://dx.doi.org/10.1186/1755-8794-6-44 |
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