Association of Nuclear-Localized Nemo-Like Kinase with Heat-Shock Protein 27 Inhibits Apoptosis in Human Breast Cancer Cells

Nemo-like kinase (NLK), a proline-directed serine/threonine kinase regulated by phosphorylation, can be localized in the cytosol or in the nucleus. Whether the localization of NLK can affect cell survival or cell apoptosis is yet to be disclosed. In the present study we found that NLK was mainly loc...

Descripción completa

Detalles Bibliográficos
Autores principales: Shaw-Hallgren, Gina, Chmielarska Masoumi, Katarzyna, Zarrizi, Reihaneh, Hellman, Ulf, Karlsson, Per, Helou, Khalil, Massoumi, Ramin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015990/
https://www.ncbi.nlm.nih.gov/pubmed/24816797
http://dx.doi.org/10.1371/journal.pone.0096506
_version_ 1782315442160795648
author Shaw-Hallgren, Gina
Chmielarska Masoumi, Katarzyna
Zarrizi, Reihaneh
Hellman, Ulf
Karlsson, Per
Helou, Khalil
Massoumi, Ramin
author_facet Shaw-Hallgren, Gina
Chmielarska Masoumi, Katarzyna
Zarrizi, Reihaneh
Hellman, Ulf
Karlsson, Per
Helou, Khalil
Massoumi, Ramin
author_sort Shaw-Hallgren, Gina
collection PubMed
description Nemo-like kinase (NLK), a proline-directed serine/threonine kinase regulated by phosphorylation, can be localized in the cytosol or in the nucleus. Whether the localization of NLK can affect cell survival or cell apoptosis is yet to be disclosed. In the present study we found that NLK was mainly localized in the nuclei of breast cancer cells, in contrast to a cytosolic localization in non-cancerous breast epithelial cells. The nuclear localization of NLK was mediated through direct interaction with Heat shock protein 27 (HSP27) which further protected cancer cells from apoptosis. The present study provides evidence of a novel mechanism by which HSP27 recognizes NLK in the breast cancer cells and prevents NLK-mediated cell apoptosis.
format Online
Article
Text
id pubmed-4015990
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-40159902014-05-14 Association of Nuclear-Localized Nemo-Like Kinase with Heat-Shock Protein 27 Inhibits Apoptosis in Human Breast Cancer Cells Shaw-Hallgren, Gina Chmielarska Masoumi, Katarzyna Zarrizi, Reihaneh Hellman, Ulf Karlsson, Per Helou, Khalil Massoumi, Ramin PLoS One Research Article Nemo-like kinase (NLK), a proline-directed serine/threonine kinase regulated by phosphorylation, can be localized in the cytosol or in the nucleus. Whether the localization of NLK can affect cell survival or cell apoptosis is yet to be disclosed. In the present study we found that NLK was mainly localized in the nuclei of breast cancer cells, in contrast to a cytosolic localization in non-cancerous breast epithelial cells. The nuclear localization of NLK was mediated through direct interaction with Heat shock protein 27 (HSP27) which further protected cancer cells from apoptosis. The present study provides evidence of a novel mechanism by which HSP27 recognizes NLK in the breast cancer cells and prevents NLK-mediated cell apoptosis. Public Library of Science 2014-05-09 /pmc/articles/PMC4015990/ /pubmed/24816797 http://dx.doi.org/10.1371/journal.pone.0096506 Text en © 2014 Shaw-Hallgren et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Shaw-Hallgren, Gina
Chmielarska Masoumi, Katarzyna
Zarrizi, Reihaneh
Hellman, Ulf
Karlsson, Per
Helou, Khalil
Massoumi, Ramin
Association of Nuclear-Localized Nemo-Like Kinase with Heat-Shock Protein 27 Inhibits Apoptosis in Human Breast Cancer Cells
title Association of Nuclear-Localized Nemo-Like Kinase with Heat-Shock Protein 27 Inhibits Apoptosis in Human Breast Cancer Cells
title_full Association of Nuclear-Localized Nemo-Like Kinase with Heat-Shock Protein 27 Inhibits Apoptosis in Human Breast Cancer Cells
title_fullStr Association of Nuclear-Localized Nemo-Like Kinase with Heat-Shock Protein 27 Inhibits Apoptosis in Human Breast Cancer Cells
title_full_unstemmed Association of Nuclear-Localized Nemo-Like Kinase with Heat-Shock Protein 27 Inhibits Apoptosis in Human Breast Cancer Cells
title_short Association of Nuclear-Localized Nemo-Like Kinase with Heat-Shock Protein 27 Inhibits Apoptosis in Human Breast Cancer Cells
title_sort association of nuclear-localized nemo-like kinase with heat-shock protein 27 inhibits apoptosis in human breast cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015990/
https://www.ncbi.nlm.nih.gov/pubmed/24816797
http://dx.doi.org/10.1371/journal.pone.0096506
work_keys_str_mv AT shawhallgrengina associationofnuclearlocalizednemolikekinasewithheatshockprotein27inhibitsapoptosisinhumanbreastcancercells
AT chmielarskamasoumikatarzyna associationofnuclearlocalizednemolikekinasewithheatshockprotein27inhibitsapoptosisinhumanbreastcancercells
AT zarrizireihaneh associationofnuclearlocalizednemolikekinasewithheatshockprotein27inhibitsapoptosisinhumanbreastcancercells
AT hellmanulf associationofnuclearlocalizednemolikekinasewithheatshockprotein27inhibitsapoptosisinhumanbreastcancercells
AT karlssonper associationofnuclearlocalizednemolikekinasewithheatshockprotein27inhibitsapoptosisinhumanbreastcancercells
AT heloukhalil associationofnuclearlocalizednemolikekinasewithheatshockprotein27inhibitsapoptosisinhumanbreastcancercells
AT massoumiramin associationofnuclearlocalizednemolikekinasewithheatshockprotein27inhibitsapoptosisinhumanbreastcancercells

Ejemplares similares