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Using (15)N-Ammonium to Characterise and Map Potassium Binding Sites in Proteins by NMR Spectroscopy
A variety of enzymes are activated by the binding of potassium ions. The potassium binding sites of these enzymes are very specific, but ammonium ions can often replace potassium ions in vitro because of their similar ionic radii. In these cases, ammonium can be used as a proxy for potassium to char...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
WILEY-VCH Verlag
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4016748/ https://www.ncbi.nlm.nih.gov/pubmed/24520048 http://dx.doi.org/10.1002/cbic.201300700 |