Tal2 expression is induced by all-trans retinoic acid in P19 cells prior to acquisition of neural fate

TAL2 is a member of the basic helix-loop-helix family and is essential for the normal development of the mouse brain. However, the function of TAL2 during brain development is unclear. P19 cells are pluripotent mouse embryonal carcinoma cells that adopt neural fates upon exposure to all-trans retino...

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Autores principales: Kobayashi, Takanobu, Komori, Rie, Ishida, Kiyoshi, Kino, Katsuhito, Tanuma, Sei-ichi, Miyazawa, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017210/
https://www.ncbi.nlm.nih.gov/pubmed/24816818
http://dx.doi.org/10.1038/srep04935
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author Kobayashi, Takanobu
Komori, Rie
Ishida, Kiyoshi
Kino, Katsuhito
Tanuma, Sei-ichi
Miyazawa, Hiroshi
author_facet Kobayashi, Takanobu
Komori, Rie
Ishida, Kiyoshi
Kino, Katsuhito
Tanuma, Sei-ichi
Miyazawa, Hiroshi
author_sort Kobayashi, Takanobu
collection PubMed
description TAL2 is a member of the basic helix-loop-helix family and is essential for the normal development of the mouse brain. However, the function of TAL2 during brain development is unclear. P19 cells are pluripotent mouse embryonal carcinoma cells that adopt neural fates upon exposure to all-trans retinoic acid (atRA) and culture in suspension. We found that the expression of Tal2 gene was induced in P19 cells after addition of atRA in suspension culture. Tal2 expression was detected within 3 h after the induction, and had nearly returned to basal levels by 24 h. When GFP-tagged TAL2 (GFP-TAL2) was expressed in P19 cells, we observed GFP-TAL2 in the nucleus. Moreover, we showed that atRA and retinoic acid receptor α regulated Tal2 expression. These results demonstrate for the first time that atRA induces Tal2 expression in P19 cells, and suggest that TAL2 commits to the acquisition of neural fate in brain development.
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spelling pubmed-40172102014-05-13 Tal2 expression is induced by all-trans retinoic acid in P19 cells prior to acquisition of neural fate Kobayashi, Takanobu Komori, Rie Ishida, Kiyoshi Kino, Katsuhito Tanuma, Sei-ichi Miyazawa, Hiroshi Sci Rep Article TAL2 is a member of the basic helix-loop-helix family and is essential for the normal development of the mouse brain. However, the function of TAL2 during brain development is unclear. P19 cells are pluripotent mouse embryonal carcinoma cells that adopt neural fates upon exposure to all-trans retinoic acid (atRA) and culture in suspension. We found that the expression of Tal2 gene was induced in P19 cells after addition of atRA in suspension culture. Tal2 expression was detected within 3 h after the induction, and had nearly returned to basal levels by 24 h. When GFP-tagged TAL2 (GFP-TAL2) was expressed in P19 cells, we observed GFP-TAL2 in the nucleus. Moreover, we showed that atRA and retinoic acid receptor α regulated Tal2 expression. These results demonstrate for the first time that atRA induces Tal2 expression in P19 cells, and suggest that TAL2 commits to the acquisition of neural fate in brain development. Nature Publishing Group 2014-05-12 /pmc/articles/PMC4017210/ /pubmed/24816818 http://dx.doi.org/10.1038/srep04935 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/
spellingShingle Article
Kobayashi, Takanobu
Komori, Rie
Ishida, Kiyoshi
Kino, Katsuhito
Tanuma, Sei-ichi
Miyazawa, Hiroshi
Tal2 expression is induced by all-trans retinoic acid in P19 cells prior to acquisition of neural fate
title Tal2 expression is induced by all-trans retinoic acid in P19 cells prior to acquisition of neural fate
title_full Tal2 expression is induced by all-trans retinoic acid in P19 cells prior to acquisition of neural fate
title_fullStr Tal2 expression is induced by all-trans retinoic acid in P19 cells prior to acquisition of neural fate
title_full_unstemmed Tal2 expression is induced by all-trans retinoic acid in P19 cells prior to acquisition of neural fate
title_short Tal2 expression is induced by all-trans retinoic acid in P19 cells prior to acquisition of neural fate
title_sort tal2 expression is induced by all-trans retinoic acid in p19 cells prior to acquisition of neural fate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017210/
https://www.ncbi.nlm.nih.gov/pubmed/24816818
http://dx.doi.org/10.1038/srep04935
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