Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran

AIM: We intended to find out the diversity of EPEC isolates among asymptomatic or diarrheal children in Iran using ribotyping. BACKGROUND: Enteropathogenic Escherichia coli (EPEC) is responsible for gastroenteritis especially in young children. PATIENTS AND METHODS: A total of 39 EPEC collected stra...

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Autores principales: Nikbin, Vajihe Sadat, Alikhani, Mohammad Yousef, Shojaei, Hasan, Mousavi, Seyed Fazlollah, Salmanzadeh-Ahrabi, Siavosh, Aslani, Mohammad Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Institute for Gastroenterology and Liver Diseases 2012
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017452/
https://www.ncbi.nlm.nih.gov/pubmed/24834207
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author Nikbin, Vajihe Sadat
Alikhani, Mohammad Yousef
Shojaei, Hasan
Mousavi, Seyed Fazlollah
Salmanzadeh-Ahrabi, Siavosh
Aslani, Mohammad Mehdi
author_facet Nikbin, Vajihe Sadat
Alikhani, Mohammad Yousef
Shojaei, Hasan
Mousavi, Seyed Fazlollah
Salmanzadeh-Ahrabi, Siavosh
Aslani, Mohammad Mehdi
author_sort Nikbin, Vajihe Sadat
collection PubMed
description AIM: We intended to find out the diversity of EPEC isolates among asymptomatic or diarrheal children in Iran using ribotyping. BACKGROUND: Enteropathogenic Escherichia coli (EPEC) is responsible for gastroenteritis especially in young children. PATIENTS AND METHODS: A total of 39 EPEC collected strains were serotyped and the presence of virulence genes as well as EAF plasmid among the strains was studied. Adherence assay was also performed. Clonal diversity of the isolates was investigated using ribotyping. RESULTS: Of 39 studied strains of E. coli, 6 serogroups of EPEC were represented. The presence of the stx gene was ascertained in 7 isolates and the eaeA, eaeB and bfpA genes were harbored by 5, 3 and 1 strains, respectively. Ribotyping yielded 9 different clusters. CONCLUSION: According to our results there was not a significant correlation between the results of serotyping and those of ribotyping. However, different serotypes of E. coli may belong to the same ribotype clusters and vice versa.
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spelling pubmed-40174522014-05-15 Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran Nikbin, Vajihe Sadat Alikhani, Mohammad Yousef Shojaei, Hasan Mousavi, Seyed Fazlollah Salmanzadeh-Ahrabi, Siavosh Aslani, Mohammad Mehdi Gastroenterol Hepatol Bed Bench Original Article AIM: We intended to find out the diversity of EPEC isolates among asymptomatic or diarrheal children in Iran using ribotyping. BACKGROUND: Enteropathogenic Escherichia coli (EPEC) is responsible for gastroenteritis especially in young children. PATIENTS AND METHODS: A total of 39 EPEC collected strains were serotyped and the presence of virulence genes as well as EAF plasmid among the strains was studied. Adherence assay was also performed. Clonal diversity of the isolates was investigated using ribotyping. RESULTS: Of 39 studied strains of E. coli, 6 serogroups of EPEC were represented. The presence of the stx gene was ascertained in 7 isolates and the eaeA, eaeB and bfpA genes were harbored by 5, 3 and 1 strains, respectively. Ribotyping yielded 9 different clusters. CONCLUSION: According to our results there was not a significant correlation between the results of serotyping and those of ribotyping. However, different serotypes of E. coli may belong to the same ribotype clusters and vice versa. Research Institute for Gastroenterology and Liver Diseases 2012 /pmc/articles/PMC4017452/ /pubmed/24834207 Text en Copyright © 2012 Research Institute for Gastroenterology and Liver Diseases http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Nikbin, Vajihe Sadat
Alikhani, Mohammad Yousef
Shojaei, Hasan
Mousavi, Seyed Fazlollah
Salmanzadeh-Ahrabi, Siavosh
Aslani, Mohammad Mehdi
Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran
title Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran
title_full Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran
title_fullStr Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran
title_full_unstemmed Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran
title_short Ribotyping of EPEC isolates from diarrheal and asymptomatic patients in Iran
title_sort ribotyping of epec isolates from diarrheal and asymptomatic patients in iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017452/
https://www.ncbi.nlm.nih.gov/pubmed/24834207
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