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Detection of Cryptosporidium infection by modified ziehl-neelsen and PCR methods in children with diarrheal samples in pediatric hospitals in Tehran

AIM: The aim of this study is evaluation of molecular assay and the standard staining method. BACKGROUND: Cryptosporidium is a protozoon from coccidian subclass, which is one of the most important causes of diarrhea in children and immunocompromised individuals around the world. Diagnosis and treatm...

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Autores principales: Tahvildar-Biderouni, Farid, Salehi, Nima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Institute for Gastroenterology and Liver Diseases 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017569/
https://www.ncbi.nlm.nih.gov/pubmed/24834304
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author Tahvildar-Biderouni, Farid
Salehi, Nima
author_facet Tahvildar-Biderouni, Farid
Salehi, Nima
author_sort Tahvildar-Biderouni, Farid
collection PubMed
description AIM: The aim of this study is evaluation of molecular assay and the standard staining method. BACKGROUND: Cryptosporidium is a protozoon from coccidian subclass, which is one of the most important causes of diarrhea in children and immunocompromised individuals around the world. Diagnosis and treatment are necessary for mentioned cases. Usual diagnostic method for this parasite is fecal smear preparation, modified ziehl-neelsen staining, microscopic consideration and oocyst observation. PATIENTS AND METHODS: A totally of 2510 stool samples collected from children with diarrhea of 4 pediatric hospitals. Direct smears prepared from fresh fecal samples and from the sediment of formalin-ether method of the same samples. The smears stained with modified ziehl-neelsen method then considered with microscope. The 30 positive samples with staining method considered with DNA extraction and PCR method for cryptosporidiosis infection determination and sensitivity evaluation. 114 random negative samples considered with DNA extraction and PCR method for cryptosporidiosis infection diagnosis and specificity evaluation. RESULTS: 30 positive cases from 2510 fecal samples detected by modified ziehl-neelsen staining and PCR method. We did not have any false positive cases by staining method but 2 cases of negative samples by staining method were positive by PCR technique, which informed us of 2 false negative. The positive samples sequenced for reconfirmation. CONCLUSION: Thus, sensitivity of staining method was computed to be 94% and specificity was 100% but sensitivity and specificity of PCR method was calculated to be 100%.
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spelling pubmed-40175692014-05-15 Detection of Cryptosporidium infection by modified ziehl-neelsen and PCR methods in children with diarrheal samples in pediatric hospitals in Tehran Tahvildar-Biderouni, Farid Salehi, Nima Gastroenterol Hepatol Bed Bench Original Article AIM: The aim of this study is evaluation of molecular assay and the standard staining method. BACKGROUND: Cryptosporidium is a protozoon from coccidian subclass, which is one of the most important causes of diarrhea in children and immunocompromised individuals around the world. Diagnosis and treatment are necessary for mentioned cases. Usual diagnostic method for this parasite is fecal smear preparation, modified ziehl-neelsen staining, microscopic consideration and oocyst observation. PATIENTS AND METHODS: A totally of 2510 stool samples collected from children with diarrhea of 4 pediatric hospitals. Direct smears prepared from fresh fecal samples and from the sediment of formalin-ether method of the same samples. The smears stained with modified ziehl-neelsen method then considered with microscope. The 30 positive samples with staining method considered with DNA extraction and PCR method for cryptosporidiosis infection determination and sensitivity evaluation. 114 random negative samples considered with DNA extraction and PCR method for cryptosporidiosis infection diagnosis and specificity evaluation. RESULTS: 30 positive cases from 2510 fecal samples detected by modified ziehl-neelsen staining and PCR method. We did not have any false positive cases by staining method but 2 cases of negative samples by staining method were positive by PCR technique, which informed us of 2 false negative. The positive samples sequenced for reconfirmation. CONCLUSION: Thus, sensitivity of staining method was computed to be 94% and specificity was 100% but sensitivity and specificity of PCR method was calculated to be 100%. Research Institute for Gastroenterology and Liver Diseases 2014 /pmc/articles/PMC4017569/ /pubmed/24834304 Text en Copyright © 2014 Research Institute for Gastroenterology and Liver Diseases http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Tahvildar-Biderouni, Farid
Salehi, Nima
Detection of Cryptosporidium infection by modified ziehl-neelsen and PCR methods in children with diarrheal samples in pediatric hospitals in Tehran
title Detection of Cryptosporidium infection by modified ziehl-neelsen and PCR methods in children with diarrheal samples in pediatric hospitals in Tehran
title_full Detection of Cryptosporidium infection by modified ziehl-neelsen and PCR methods in children with diarrheal samples in pediatric hospitals in Tehran
title_fullStr Detection of Cryptosporidium infection by modified ziehl-neelsen and PCR methods in children with diarrheal samples in pediatric hospitals in Tehran
title_full_unstemmed Detection of Cryptosporidium infection by modified ziehl-neelsen and PCR methods in children with diarrheal samples in pediatric hospitals in Tehran
title_short Detection of Cryptosporidium infection by modified ziehl-neelsen and PCR methods in children with diarrheal samples in pediatric hospitals in Tehran
title_sort detection of cryptosporidium infection by modified ziehl-neelsen and pcr methods in children with diarrheal samples in pediatric hospitals in tehran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017569/
https://www.ncbi.nlm.nih.gov/pubmed/24834304
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