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Cellular Contact Guidance through Dynamic Sensing of Nanotopography

[Image: see text] We investigate the effects of surface nanotopography on the migration and cell shape dynamics of the amoeba Dictyostelium discoideum. Multiple prior studies have implicated the patterning of focal adhesions in contact guidance. However, we observe significant contact guidance of Di...

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Autores principales: Driscoll, Meghan K., Sun, Xiaoyu, Guven, Can, Fourkas, John T., Losert, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017610/
https://www.ncbi.nlm.nih.gov/pubmed/24649900
http://dx.doi.org/10.1021/nn406637c
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author Driscoll, Meghan K.
Sun, Xiaoyu
Guven, Can
Fourkas, John T.
Losert, Wolfgang
author_facet Driscoll, Meghan K.
Sun, Xiaoyu
Guven, Can
Fourkas, John T.
Losert, Wolfgang
author_sort Driscoll, Meghan K.
collection PubMed
description [Image: see text] We investigate the effects of surface nanotopography on the migration and cell shape dynamics of the amoeba Dictyostelium discoideum. Multiple prior studies have implicated the patterning of focal adhesions in contact guidance. However, we observe significant contact guidance of Dictyostelium along surfaces with nanoscale ridges or grooves, even though this organism lacks integrin-based adhesions. Cells that move parallel to nanoridges are faster, more protrusive at their fronts, and more elongated than are cells that move perpendicular to nanoridges. Quantitative studies show that nanoridges spaced 1.5 μm apart exhibit the greatest contact guidance efficiency. Because Dictyostelium cells exhibit oscillatory shape dynamics, we model contact guidance as a process in which stochastic cellular harmonic oscillators couple to the periodicity of the nanoridges. In support of this connection, we find that nanoridges nucleate actin polymerization waves of nanoscale width that propagate parallel to the nanoridges.
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spelling pubmed-40176102015-03-20 Cellular Contact Guidance through Dynamic Sensing of Nanotopography Driscoll, Meghan K. Sun, Xiaoyu Guven, Can Fourkas, John T. Losert, Wolfgang ACS Nano [Image: see text] We investigate the effects of surface nanotopography on the migration and cell shape dynamics of the amoeba Dictyostelium discoideum. Multiple prior studies have implicated the patterning of focal adhesions in contact guidance. However, we observe significant contact guidance of Dictyostelium along surfaces with nanoscale ridges or grooves, even though this organism lacks integrin-based adhesions. Cells that move parallel to nanoridges are faster, more protrusive at their fronts, and more elongated than are cells that move perpendicular to nanoridges. Quantitative studies show that nanoridges spaced 1.5 μm apart exhibit the greatest contact guidance efficiency. Because Dictyostelium cells exhibit oscillatory shape dynamics, we model contact guidance as a process in which stochastic cellular harmonic oscillators couple to the periodicity of the nanoridges. In support of this connection, we find that nanoridges nucleate actin polymerization waves of nanoscale width that propagate parallel to the nanoridges. American Chemical Society 2014-03-20 2014-04-22 /pmc/articles/PMC4017610/ /pubmed/24649900 http://dx.doi.org/10.1021/nn406637c Text en Copyright © 2014 American Chemical Society
spellingShingle Driscoll, Meghan K.
Sun, Xiaoyu
Guven, Can
Fourkas, John T.
Losert, Wolfgang
Cellular Contact Guidance through Dynamic Sensing of Nanotopography
title Cellular Contact Guidance through Dynamic Sensing of Nanotopography
title_full Cellular Contact Guidance through Dynamic Sensing of Nanotopography
title_fullStr Cellular Contact Guidance through Dynamic Sensing of Nanotopography
title_full_unstemmed Cellular Contact Guidance through Dynamic Sensing of Nanotopography
title_short Cellular Contact Guidance through Dynamic Sensing of Nanotopography
title_sort cellular contact guidance through dynamic sensing of nanotopography
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017610/
https://www.ncbi.nlm.nih.gov/pubmed/24649900
http://dx.doi.org/10.1021/nn406637c
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