Cargando…

Leptospira spp. infection in sheep herds in southeast Brazil

BACKGROUND: With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, São Paulo state, southeast Brazil. The mi...

Descripción completa

Detalles Bibliográficos
Autores principales: Barbante, Priscila, Shimabukuro, Fabio H, Langoni, Helio, Richini-Pereira, Virgínia B, Lucheis, Simone B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017680/
https://www.ncbi.nlm.nih.gov/pubmed/24822059
http://dx.doi.org/10.1186/1678-9199-20-20
_version_ 1782479990394191872
author Barbante, Priscila
Shimabukuro, Fabio H
Langoni, Helio
Richini-Pereira, Virgínia B
Lucheis, Simone B
author_facet Barbante, Priscila
Shimabukuro, Fabio H
Langoni, Helio
Richini-Pereira, Virgínia B
Lucheis, Simone B
author_sort Barbante, Priscila
collection PubMed
description BACKGROUND: With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, São Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp. RESULTS: MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys and four livers), corresponding to five animals with positive serology (one animal simultaneously positive for both kidney and liver) and two negatives. PCR detected Leptospira spp. in 14 samples (seven kidneys and seven livers) corresponding to 12 positive animals (two animals simultaneously positive for kidney and liver), of which ten were serologically positive and two negative. CONCLUSIONS: PCR was faster, more practical and more sensitive than culture for detecting leptospires. The results reinforce the importance of sheep in the epidemiological context of leptospirosis.
format Online
Article
Text
id pubmed-4017680
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-40176802014-05-13 Leptospira spp. infection in sheep herds in southeast Brazil Barbante, Priscila Shimabukuro, Fabio H Langoni, Helio Richini-Pereira, Virgínia B Lucheis, Simone B J Venom Anim Toxins Incl Trop Dis Research BACKGROUND: With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, São Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp. RESULTS: MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys and four livers), corresponding to five animals with positive serology (one animal simultaneously positive for both kidney and liver) and two negatives. PCR detected Leptospira spp. in 14 samples (seven kidneys and seven livers) corresponding to 12 positive animals (two animals simultaneously positive for kidney and liver), of which ten were serologically positive and two negative. CONCLUSIONS: PCR was faster, more practical and more sensitive than culture for detecting leptospires. The results reinforce the importance of sheep in the epidemiological context of leptospirosis. BioMed Central 2014-05-06 /pmc/articles/PMC4017680/ /pubmed/24822059 http://dx.doi.org/10.1186/1678-9199-20-20 Text en Copyright © 2014 Barbante et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Barbante, Priscila
Shimabukuro, Fabio H
Langoni, Helio
Richini-Pereira, Virgínia B
Lucheis, Simone B
Leptospira spp. infection in sheep herds in southeast Brazil
title Leptospira spp. infection in sheep herds in southeast Brazil
title_full Leptospira spp. infection in sheep herds in southeast Brazil
title_fullStr Leptospira spp. infection in sheep herds in southeast Brazil
title_full_unstemmed Leptospira spp. infection in sheep herds in southeast Brazil
title_short Leptospira spp. infection in sheep herds in southeast Brazil
title_sort leptospira spp. infection in sheep herds in southeast brazil
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017680/
https://www.ncbi.nlm.nih.gov/pubmed/24822059
http://dx.doi.org/10.1186/1678-9199-20-20
work_keys_str_mv AT barbantepriscila leptospirasppinfectioninsheepherdsinsoutheastbrazil
AT shimabukurofabioh leptospirasppinfectioninsheepherdsinsoutheastbrazil
AT langonihelio leptospirasppinfectioninsheepherdsinsoutheastbrazil
AT richinipereiravirginiab leptospirasppinfectioninsheepherdsinsoutheastbrazil
AT lucheissimoneb leptospirasppinfectioninsheepherdsinsoutheastbrazil