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Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease
Background. Recent reports suggest a role for the endocannabinoid system in the pathology of nonalcoholic fatty liver disease (NAFLD). The aim of this study was to investigate the relationship between liver expression of cannabinoid (CB) receptor subtypes, CB1 and CB2, in morbidly obese (MO) women w...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017805/ https://www.ncbi.nlm.nih.gov/pubmed/24864249 http://dx.doi.org/10.1155/2014/502542 |
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author | Auguet, Teresa Berlanga, Alba Guiu-Jurado, Esther Terra, Ximena Martinez, Salomé Aguilar, Carmen Filiu, Elisa Alibalic, Ajla Sabench, Fàtima Hernández, Mercé Del Castillo, Daniel Richart, Cristóbal |
author_facet | Auguet, Teresa Berlanga, Alba Guiu-Jurado, Esther Terra, Ximena Martinez, Salomé Aguilar, Carmen Filiu, Elisa Alibalic, Ajla Sabench, Fàtima Hernández, Mercé Del Castillo, Daniel Richart, Cristóbal |
author_sort | Auguet, Teresa |
collection | PubMed |
description | Background. Recent reports suggest a role for the endocannabinoid system in the pathology of nonalcoholic fatty liver disease (NAFLD). The aim of this study was to investigate the relationship between liver expression of cannabinoid (CB) receptor subtypes, CB1 and CB2, in morbidly obese (MO) women with different histological stages of NAFLD. Methods. We analysed hepatic CB1 and CB2 mRNA expression, and the expression of genes involved in lipid metabolism in 72 MO women, subclassified by liver histology into MO with normal liver (NL, n = 16), simple steatosis (SS, n = 28), and nonalcoholic steatohepatitis (NASH, n = 28) by enzyme-linked immunosorbent assay and RT-PCR. Results. We found that CB1 mRNA expression was significantly higher in NASH compared with SS and correlated negatively with PPARα. Regarding CB2, CB2 mRNA expression correlated positively with ACC1, PPARγ, IL6, TNFα, resistin, and adiponectin. Conclusions. The increased expression of CB1 in NASH and the negative correlation with PPARα suggest a deleterious role of CB1 in NAFLD. Regarding CB2, its positive correlation with the anti-inflammatory molecule adiponectin and, paradoxically, with inflammatory genes suggests that this receptor has a dual role. Taken together, our results suggest that endocannabinoid receptors might be involved in the pathogenesis of NAFLD, a finding which justifies further study. |
format | Online Article Text |
id | pubmed-4017805 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-40178052014-05-26 Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease Auguet, Teresa Berlanga, Alba Guiu-Jurado, Esther Terra, Ximena Martinez, Salomé Aguilar, Carmen Filiu, Elisa Alibalic, Ajla Sabench, Fàtima Hernández, Mercé Del Castillo, Daniel Richart, Cristóbal Biomed Res Int Research Article Background. Recent reports suggest a role for the endocannabinoid system in the pathology of nonalcoholic fatty liver disease (NAFLD). The aim of this study was to investigate the relationship between liver expression of cannabinoid (CB) receptor subtypes, CB1 and CB2, in morbidly obese (MO) women with different histological stages of NAFLD. Methods. We analysed hepatic CB1 and CB2 mRNA expression, and the expression of genes involved in lipid metabolism in 72 MO women, subclassified by liver histology into MO with normal liver (NL, n = 16), simple steatosis (SS, n = 28), and nonalcoholic steatohepatitis (NASH, n = 28) by enzyme-linked immunosorbent assay and RT-PCR. Results. We found that CB1 mRNA expression was significantly higher in NASH compared with SS and correlated negatively with PPARα. Regarding CB2, CB2 mRNA expression correlated positively with ACC1, PPARγ, IL6, TNFα, resistin, and adiponectin. Conclusions. The increased expression of CB1 in NASH and the negative correlation with PPARα suggest a deleterious role of CB1 in NAFLD. Regarding CB2, its positive correlation with the anti-inflammatory molecule adiponectin and, paradoxically, with inflammatory genes suggests that this receptor has a dual role. Taken together, our results suggest that endocannabinoid receptors might be involved in the pathogenesis of NAFLD, a finding which justifies further study. Hindawi Publishing Corporation 2014 2014-04-23 /pmc/articles/PMC4017805/ /pubmed/24864249 http://dx.doi.org/10.1155/2014/502542 Text en Copyright © 2014 Teresa Auguet et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Auguet, Teresa Berlanga, Alba Guiu-Jurado, Esther Terra, Ximena Martinez, Salomé Aguilar, Carmen Filiu, Elisa Alibalic, Ajla Sabench, Fàtima Hernández, Mercé Del Castillo, Daniel Richart, Cristóbal Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease |
title | Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease |
title_full | Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease |
title_fullStr | Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease |
title_full_unstemmed | Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease |
title_short | Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease |
title_sort | endocannabinoid receptors gene expression in morbidly obese women with nonalcoholic fatty liver disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017805/ https://www.ncbi.nlm.nih.gov/pubmed/24864249 http://dx.doi.org/10.1155/2014/502542 |
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