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Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease

Background. Recent reports suggest a role for the endocannabinoid system in the pathology of nonalcoholic fatty liver disease (NAFLD). The aim of this study was to investigate the relationship between liver expression of cannabinoid (CB) receptor subtypes, CB1 and CB2, in morbidly obese (MO) women w...

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Autores principales: Auguet, Teresa, Berlanga, Alba, Guiu-Jurado, Esther, Terra, Ximena, Martinez, Salomé, Aguilar, Carmen, Filiu, Elisa, Alibalic, Ajla, Sabench, Fàtima, Hernández, Mercé, Del Castillo, Daniel, Richart, Cristóbal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017805/
https://www.ncbi.nlm.nih.gov/pubmed/24864249
http://dx.doi.org/10.1155/2014/502542
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author Auguet, Teresa
Berlanga, Alba
Guiu-Jurado, Esther
Terra, Ximena
Martinez, Salomé
Aguilar, Carmen
Filiu, Elisa
Alibalic, Ajla
Sabench, Fàtima
Hernández, Mercé
Del Castillo, Daniel
Richart, Cristóbal
author_facet Auguet, Teresa
Berlanga, Alba
Guiu-Jurado, Esther
Terra, Ximena
Martinez, Salomé
Aguilar, Carmen
Filiu, Elisa
Alibalic, Ajla
Sabench, Fàtima
Hernández, Mercé
Del Castillo, Daniel
Richart, Cristóbal
author_sort Auguet, Teresa
collection PubMed
description Background. Recent reports suggest a role for the endocannabinoid system in the pathology of nonalcoholic fatty liver disease (NAFLD). The aim of this study was to investigate the relationship between liver expression of cannabinoid (CB) receptor subtypes, CB1 and CB2, in morbidly obese (MO) women with different histological stages of NAFLD. Methods. We analysed hepatic CB1 and CB2 mRNA expression, and the expression of genes involved in lipid metabolism in 72 MO women, subclassified by liver histology into MO with normal liver (NL, n = 16), simple steatosis (SS, n = 28), and nonalcoholic steatohepatitis (NASH, n = 28) by enzyme-linked immunosorbent assay and RT-PCR. Results. We found that CB1 mRNA expression was significantly higher in NASH compared with SS and correlated negatively with PPARα. Regarding CB2, CB2 mRNA expression correlated positively with ACC1, PPARγ, IL6, TNFα, resistin, and adiponectin. Conclusions. The increased expression of CB1 in NASH and the negative correlation with PPARα suggest a deleterious role of CB1 in NAFLD. Regarding CB2, its positive correlation with the anti-inflammatory molecule adiponectin and, paradoxically, with inflammatory genes suggests that this receptor has a dual role. Taken together, our results suggest that endocannabinoid receptors might be involved in the pathogenesis of NAFLD, a finding which justifies further study.
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spelling pubmed-40178052014-05-26 Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease Auguet, Teresa Berlanga, Alba Guiu-Jurado, Esther Terra, Ximena Martinez, Salomé Aguilar, Carmen Filiu, Elisa Alibalic, Ajla Sabench, Fàtima Hernández, Mercé Del Castillo, Daniel Richart, Cristóbal Biomed Res Int Research Article Background. Recent reports suggest a role for the endocannabinoid system in the pathology of nonalcoholic fatty liver disease (NAFLD). The aim of this study was to investigate the relationship between liver expression of cannabinoid (CB) receptor subtypes, CB1 and CB2, in morbidly obese (MO) women with different histological stages of NAFLD. Methods. We analysed hepatic CB1 and CB2 mRNA expression, and the expression of genes involved in lipid metabolism in 72 MO women, subclassified by liver histology into MO with normal liver (NL, n = 16), simple steatosis (SS, n = 28), and nonalcoholic steatohepatitis (NASH, n = 28) by enzyme-linked immunosorbent assay and RT-PCR. Results. We found that CB1 mRNA expression was significantly higher in NASH compared with SS and correlated negatively with PPARα. Regarding CB2, CB2 mRNA expression correlated positively with ACC1, PPARγ, IL6, TNFα, resistin, and adiponectin. Conclusions. The increased expression of CB1 in NASH and the negative correlation with PPARα suggest a deleterious role of CB1 in NAFLD. Regarding CB2, its positive correlation with the anti-inflammatory molecule adiponectin and, paradoxically, with inflammatory genes suggests that this receptor has a dual role. Taken together, our results suggest that endocannabinoid receptors might be involved in the pathogenesis of NAFLD, a finding which justifies further study. Hindawi Publishing Corporation 2014 2014-04-23 /pmc/articles/PMC4017805/ /pubmed/24864249 http://dx.doi.org/10.1155/2014/502542 Text en Copyright © 2014 Teresa Auguet et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Auguet, Teresa
Berlanga, Alba
Guiu-Jurado, Esther
Terra, Ximena
Martinez, Salomé
Aguilar, Carmen
Filiu, Elisa
Alibalic, Ajla
Sabench, Fàtima
Hernández, Mercé
Del Castillo, Daniel
Richart, Cristóbal
Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease
title Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease
title_full Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease
title_fullStr Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease
title_full_unstemmed Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease
title_short Endocannabinoid Receptors Gene Expression in Morbidly Obese Women with Nonalcoholic Fatty Liver Disease
title_sort endocannabinoid receptors gene expression in morbidly obese women with nonalcoholic fatty liver disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4017805/
https://www.ncbi.nlm.nih.gov/pubmed/24864249
http://dx.doi.org/10.1155/2014/502542
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