Cargando…
Antibiotics supplemented culture media can eliminate non-specific bacteria from human semen during sperm preparation for intra uterine insemination
RATIONALE: Bacterial flora can be isolated from many semen samples of subfertile males. Bacteriospermia can compromise the outcome of intra uterine insemination (IUI) by contaminating the post-processed sperm sample. OBJECTIVES: The objective of the present study is to determine the efficacy of peni...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4018800/ https://www.ncbi.nlm.nih.gov/pubmed/24829533 http://dx.doi.org/10.4103/0974-1208.130859 |
_version_ | 1782480127191416832 |
---|---|
author | Dissanayake, D. M. A. B. Amaranath, K A Perera, R. R. D. P. Wijesinghe, P S |
author_facet | Dissanayake, D. M. A. B. Amaranath, K A Perera, R. R. D. P. Wijesinghe, P S |
author_sort | Dissanayake, D. M. A. B. |
collection | PubMed |
description | RATIONALE: Bacterial flora can be isolated from many semen samples of subfertile males. Bacteriospermia can compromise the outcome of intra uterine insemination (IUI) by contaminating the post-processed sperm sample. OBJECTIVES: The objective of the present study is to determine the efficacy of penicillin and streptomycin in eliminating the bacteria from semen samples in the sperm processing procedure, and to assess the effects of antibiotics on sperm motility, survivability, and pregnancy rates. DESIGN AND SETTINGS: A prospectively controlled study was carried out using couples undergoing IUI with their informed consent. INTERVENTION: Sperm processing using the swim-up technique in penicillin and streptomycin supplemented culture medium. SUBJECTS AND METHODS: Couples were consecutively allocated in two groups for sperm processing (a) Group AB+ (antibiotics supplemented culture medium, n = 33) and (b) Group AB− (antibiotic free culture medium, n = 33). Semen culture was performed before and after sperm processing. Sperm motility was assessed immediately after processing and after 24 h of incubation. RESULTS: Bacterial isolates were found in 20 (60.6%) and 22 (66.1%) of samples before processing in Groups AB+ and AB− respectively. Addition of antibiotics resulted in completely eliminating non-specific bacteria from semen samples without affecting sperm motility. In vitro survival rate of sperm enhanced in AB+ group compared with AB− group (motile sperm after 24 h), 62.21% (standard deviation [SD]: 37.27) versus 41.36% (SD: 30.78), P = 0.012. Pregnancy rate, was comparable between two groups (9% in Group AB+ vs. 6% in Group AB−, P = 0.45). CONCLUSION: Penicillin streptomycin combination could completely eliminate non-specific bacteria from semen samples during sperm processing in this population. The types of antibiotics and dosage used did not seem to have any harmful effects on human sperm. |
format | Online Article Text |
id | pubmed-4018800 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-40188002014-05-14 Antibiotics supplemented culture media can eliminate non-specific bacteria from human semen during sperm preparation for intra uterine insemination Dissanayake, D. M. A. B. Amaranath, K A Perera, R. R. D. P. Wijesinghe, P S J Hum Reprod Sci Original Article RATIONALE: Bacterial flora can be isolated from many semen samples of subfertile males. Bacteriospermia can compromise the outcome of intra uterine insemination (IUI) by contaminating the post-processed sperm sample. OBJECTIVES: The objective of the present study is to determine the efficacy of penicillin and streptomycin in eliminating the bacteria from semen samples in the sperm processing procedure, and to assess the effects of antibiotics on sperm motility, survivability, and pregnancy rates. DESIGN AND SETTINGS: A prospectively controlled study was carried out using couples undergoing IUI with their informed consent. INTERVENTION: Sperm processing using the swim-up technique in penicillin and streptomycin supplemented culture medium. SUBJECTS AND METHODS: Couples were consecutively allocated in two groups for sperm processing (a) Group AB+ (antibiotics supplemented culture medium, n = 33) and (b) Group AB− (antibiotic free culture medium, n = 33). Semen culture was performed before and after sperm processing. Sperm motility was assessed immediately after processing and after 24 h of incubation. RESULTS: Bacterial isolates were found in 20 (60.6%) and 22 (66.1%) of samples before processing in Groups AB+ and AB− respectively. Addition of antibiotics resulted in completely eliminating non-specific bacteria from semen samples without affecting sperm motility. In vitro survival rate of sperm enhanced in AB+ group compared with AB− group (motile sperm after 24 h), 62.21% (standard deviation [SD]: 37.27) versus 41.36% (SD: 30.78), P = 0.012. Pregnancy rate, was comparable between two groups (9% in Group AB+ vs. 6% in Group AB−, P = 0.45). CONCLUSION: Penicillin streptomycin combination could completely eliminate non-specific bacteria from semen samples during sperm processing in this population. The types of antibiotics and dosage used did not seem to have any harmful effects on human sperm. Medknow Publications & Media Pvt Ltd 2014 /pmc/articles/PMC4018800/ /pubmed/24829533 http://dx.doi.org/10.4103/0974-1208.130859 Text en Copyright: © Journal of Human Reproductive Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Dissanayake, D. M. A. B. Amaranath, K A Perera, R. R. D. P. Wijesinghe, P S Antibiotics supplemented culture media can eliminate non-specific bacteria from human semen during sperm preparation for intra uterine insemination |
title | Antibiotics supplemented culture media can eliminate non-specific bacteria from human semen during sperm preparation for intra uterine insemination |
title_full | Antibiotics supplemented culture media can eliminate non-specific bacteria from human semen during sperm preparation for intra uterine insemination |
title_fullStr | Antibiotics supplemented culture media can eliminate non-specific bacteria from human semen during sperm preparation for intra uterine insemination |
title_full_unstemmed | Antibiotics supplemented culture media can eliminate non-specific bacteria from human semen during sperm preparation for intra uterine insemination |
title_short | Antibiotics supplemented culture media can eliminate non-specific bacteria from human semen during sperm preparation for intra uterine insemination |
title_sort | antibiotics supplemented culture media can eliminate non-specific bacteria from human semen during sperm preparation for intra uterine insemination |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4018800/ https://www.ncbi.nlm.nih.gov/pubmed/24829533 http://dx.doi.org/10.4103/0974-1208.130859 |
work_keys_str_mv | AT dissanayakedmab antibioticssupplementedculturemediacaneliminatenonspecificbacteriafromhumansemenduringspermpreparationforintrauterineinsemination AT amaranathka antibioticssupplementedculturemediacaneliminatenonspecificbacteriafromhumansemenduringspermpreparationforintrauterineinsemination AT pererarrdp antibioticssupplementedculturemediacaneliminatenonspecificbacteriafromhumansemenduringspermpreparationforintrauterineinsemination AT wijesingheps antibioticssupplementedculturemediacaneliminatenonspecificbacteriafromhumansemenduringspermpreparationforintrauterineinsemination |