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The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis

BACKGROUND: Sepsis is a prevalent condition in critically ill patients and may be associated with thiamine deficiency (TD). The aim of this study was to evaluate the effect of TD on inflammation, oxidative stress and cellular recruitment in a sepsis model. METHODS: The experimental sepsis model, cec...

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Autores principales: de Andrade, José Antenor Araújo, Gayer, Carlos Roberto Machado, Nogueira, Natália Pereira de Almeida, Paes, Márcia Cristina, Bastos, Vera Lúcia Freire Cunha, Neto, Jayme da Cunha Bastos, Alves, Sílvio Caetano, Coelho, Raphael Molinaro, da Cunha, Mariana Gysele Amarante Teixeira, Gomes, Rachel Novaes, Águila, Márcia Barbosa, Mandarim-de-Lacerda, Carlos Alberto, Bozza, Patrícia Torres, da Cunha, Sérgio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4018973/
https://www.ncbi.nlm.nih.gov/pubmed/24826081
http://dx.doi.org/10.1186/1476-9255-11-11
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author de Andrade, José Antenor Araújo
Gayer, Carlos Roberto Machado
Nogueira, Natália Pereira de Almeida
Paes, Márcia Cristina
Bastos, Vera Lúcia Freire Cunha
Neto, Jayme da Cunha Bastos
Alves, Sílvio Caetano
Coelho, Raphael Molinaro
da Cunha, Mariana Gysele Amarante Teixeira
Gomes, Rachel Novaes
Águila, Márcia Barbosa
Mandarim-de-Lacerda, Carlos Alberto
Bozza, Patrícia Torres
da Cunha, Sérgio
author_facet de Andrade, José Antenor Araújo
Gayer, Carlos Roberto Machado
Nogueira, Natália Pereira de Almeida
Paes, Márcia Cristina
Bastos, Vera Lúcia Freire Cunha
Neto, Jayme da Cunha Bastos
Alves, Sílvio Caetano
Coelho, Raphael Molinaro
da Cunha, Mariana Gysele Amarante Teixeira
Gomes, Rachel Novaes
Águila, Márcia Barbosa
Mandarim-de-Lacerda, Carlos Alberto
Bozza, Patrícia Torres
da Cunha, Sérgio
author_sort de Andrade, José Antenor Araújo
collection PubMed
description BACKGROUND: Sepsis is a prevalent condition in critically ill patients and may be associated with thiamine deficiency (TD). The aim of this study was to evaluate the effect of TD on inflammation, oxidative stress and cellular recruitment in a sepsis model. METHODS: The experimental sepsis model, cecal ligation and puncture (CLP), was utilized on mice in comparison with a sham procedure. The following four groups were compared against each other: SHAM with AIN93G complete chow, SHAM with thiamine deficient (TD) chow, CLP with AIN93G complete chow, and CLP with TD chow. Thiamine pyrophosphate (TPP) blood concentrations were determined, and blood and peritoneal fluid were evaluated for differences in TNF-alpha, IL-1, IL-6, KC and MCP-1/CCL2 levels. In addition, the levels of 4-HNE adducts in liver proteins were evaluated by Western Blot. RESULTS: The mean TPP blood concentration from the mice fed with the complete chow was 303.3 ± 42.6 nmol/L, and TD occurred within 10 days. TNF-α and MCP-1 concentrations in the peritoneal fluid were significantly greater in the CLP with TD chow group when compared with the other groups. The blood IL-1β level, however, was lower in the CLP with TD chow group. Liver 4-HNE levels were highest in the TD chow groups. Blood mononuclear cell numbers, as well as peritoneal total leukocyte, mononuclear cell and neutrophil numbers were greater in the CLP with TD chow group. Peritoneal bacterial colony forming units (CFU) were significantly lower in the CLP with TD chow group. CONCLUSION: TD was associated with greater bacterial clearance, oxidative stress and inflammatory response changes.
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spelling pubmed-40189732014-05-14 The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis de Andrade, José Antenor Araújo Gayer, Carlos Roberto Machado Nogueira, Natália Pereira de Almeida Paes, Márcia Cristina Bastos, Vera Lúcia Freire Cunha Neto, Jayme da Cunha Bastos Alves, Sílvio Caetano Coelho, Raphael Molinaro da Cunha, Mariana Gysele Amarante Teixeira Gomes, Rachel Novaes Águila, Márcia Barbosa Mandarim-de-Lacerda, Carlos Alberto Bozza, Patrícia Torres da Cunha, Sérgio J Inflamm (Lond) Research BACKGROUND: Sepsis is a prevalent condition in critically ill patients and may be associated with thiamine deficiency (TD). The aim of this study was to evaluate the effect of TD on inflammation, oxidative stress and cellular recruitment in a sepsis model. METHODS: The experimental sepsis model, cecal ligation and puncture (CLP), was utilized on mice in comparison with a sham procedure. The following four groups were compared against each other: SHAM with AIN93G complete chow, SHAM with thiamine deficient (TD) chow, CLP with AIN93G complete chow, and CLP with TD chow. Thiamine pyrophosphate (TPP) blood concentrations were determined, and blood and peritoneal fluid were evaluated for differences in TNF-alpha, IL-1, IL-6, KC and MCP-1/CCL2 levels. In addition, the levels of 4-HNE adducts in liver proteins were evaluated by Western Blot. RESULTS: The mean TPP blood concentration from the mice fed with the complete chow was 303.3 ± 42.6 nmol/L, and TD occurred within 10 days. TNF-α and MCP-1 concentrations in the peritoneal fluid were significantly greater in the CLP with TD chow group when compared with the other groups. The blood IL-1β level, however, was lower in the CLP with TD chow group. Liver 4-HNE levels were highest in the TD chow groups. Blood mononuclear cell numbers, as well as peritoneal total leukocyte, mononuclear cell and neutrophil numbers were greater in the CLP with TD chow group. Peritoneal bacterial colony forming units (CFU) were significantly lower in the CLP with TD chow group. CONCLUSION: TD was associated with greater bacterial clearance, oxidative stress and inflammatory response changes. BioMed Central 2014-04-24 /pmc/articles/PMC4018973/ /pubmed/24826081 http://dx.doi.org/10.1186/1476-9255-11-11 Text en Copyright © 2014 de Andrade et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Research
de Andrade, José Antenor Araújo
Gayer, Carlos Roberto Machado
Nogueira, Natália Pereira de Almeida
Paes, Márcia Cristina
Bastos, Vera Lúcia Freire Cunha
Neto, Jayme da Cunha Bastos
Alves, Sílvio Caetano
Coelho, Raphael Molinaro
da Cunha, Mariana Gysele Amarante Teixeira
Gomes, Rachel Novaes
Águila, Márcia Barbosa
Mandarim-de-Lacerda, Carlos Alberto
Bozza, Patrícia Torres
da Cunha, Sérgio
The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis
title The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis
title_full The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis
title_fullStr The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis
title_full_unstemmed The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis
title_short The effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis
title_sort effect of thiamine deficiency on inflammation, oxidative stress and cellular migration in an experimental model of sepsis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4018973/
https://www.ncbi.nlm.nih.gov/pubmed/24826081
http://dx.doi.org/10.1186/1476-9255-11-11
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