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Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion

Aims/Introduction:  Excessive intake of sucrose can cause severe health issues, such as diabetes mellitus. In animal studies, consumption of a high‐sucrose diet (SUC) has been shown to cause obesity, insulin resistance and glucose intolerance. However, several in vivo experiments have been carried o...

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Autores principales: Sakamoto, Eriko, Seino, Yusuke, Fukami, Ayako, Mizutani, Naohiro, Tsunekawa, Shin, Ishikawa, Kota, Ogata, Hidetada, Uenishi, Eita, Kamiya, Hideki, Hamada, Yoji, Sato, Hiroyuki, Harada, Norio, Toyoda, Yukiyasu, Miwa, Ichitomo, Nakamura, Jiro, Inagaki, Nobuya, Oiso, Yutaka, Ozaki, Nobuaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019243/
https://www.ncbi.nlm.nih.gov/pubmed/24843603
http://dx.doi.org/10.1111/j.2040-1124.2012.00208.x
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author Sakamoto, Eriko
Seino, Yusuke
Fukami, Ayako
Mizutani, Naohiro
Tsunekawa, Shin
Ishikawa, Kota
Ogata, Hidetada
Uenishi, Eita
Kamiya, Hideki
Hamada, Yoji
Sato, Hiroyuki
Harada, Norio
Toyoda, Yukiyasu
Miwa, Ichitomo
Nakamura, Jiro
Inagaki, Nobuya
Oiso, Yutaka
Ozaki, Nobuaki
author_facet Sakamoto, Eriko
Seino, Yusuke
Fukami, Ayako
Mizutani, Naohiro
Tsunekawa, Shin
Ishikawa, Kota
Ogata, Hidetada
Uenishi, Eita
Kamiya, Hideki
Hamada, Yoji
Sato, Hiroyuki
Harada, Norio
Toyoda, Yukiyasu
Miwa, Ichitomo
Nakamura, Jiro
Inagaki, Nobuya
Oiso, Yutaka
Ozaki, Nobuaki
author_sort Sakamoto, Eriko
collection PubMed
description Aims/Introduction:  Excessive intake of sucrose can cause severe health issues, such as diabetes mellitus. In animal studies, consumption of a high‐sucrose diet (SUC) has been shown to cause obesity, insulin resistance and glucose intolerance. However, several in vivo experiments have been carried out using diets with much higher sucrose contents (50–70% of the total calories) than are typically ingested by humans. In the present study, we examined the effects of a moderate SUC on glucose metabolism and the underlying mechanism. Materials and Methods:  C57BL/6J mice received a SUC (38.5% sucrose), a high‐starch diet (ST) or a control diet for 5 weeks. We assessed glucose tolerance, incretin secretion and liver glucose metabolism. Results:  An oral glucose tolerance test (OGTT) showed that plasma glucose levels in the early phase were significantly higher in SUC‐fed mice than in ST‐fed or control mice, with no change in plasma insulin levels at any stage. SUC‐fed mice showed a significant improvement in insulin sensitivity. Glucagon‐like peptide‐1 (GLP‐1) secretion 15 min after oral glucose administration was significantly lower in SUC‐fed mice than in ST‐fed or control mice. Hepatic glucokinase (GCK) activity was significantly reduced in SUC‐fed mice. During the OGTT, the accumulation of glycogen in the liver was suppressed in SUC‐fed mice in a time‐dependent manner. Conclusions:  These results indicate that mice that consume a moderate SUC show glucose intolerance with a reduction in hepatic GCK activity and impairment in GLP‐1 secretion. (J Diabetes Invest, doi: 10.1111/j.2040‐1124.2012.00208.x, 2012)
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spelling pubmed-40192432014-05-19 Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion Sakamoto, Eriko Seino, Yusuke Fukami, Ayako Mizutani, Naohiro Tsunekawa, Shin Ishikawa, Kota Ogata, Hidetada Uenishi, Eita Kamiya, Hideki Hamada, Yoji Sato, Hiroyuki Harada, Norio Toyoda, Yukiyasu Miwa, Ichitomo Nakamura, Jiro Inagaki, Nobuya Oiso, Yutaka Ozaki, Nobuaki J Diabetes Investig Articles Aims/Introduction:  Excessive intake of sucrose can cause severe health issues, such as diabetes mellitus. In animal studies, consumption of a high‐sucrose diet (SUC) has been shown to cause obesity, insulin resistance and glucose intolerance. However, several in vivo experiments have been carried out using diets with much higher sucrose contents (50–70% of the total calories) than are typically ingested by humans. In the present study, we examined the effects of a moderate SUC on glucose metabolism and the underlying mechanism. Materials and Methods:  C57BL/6J mice received a SUC (38.5% sucrose), a high‐starch diet (ST) or a control diet for 5 weeks. We assessed glucose tolerance, incretin secretion and liver glucose metabolism. Results:  An oral glucose tolerance test (OGTT) showed that plasma glucose levels in the early phase were significantly higher in SUC‐fed mice than in ST‐fed or control mice, with no change in plasma insulin levels at any stage. SUC‐fed mice showed a significant improvement in insulin sensitivity. Glucagon‐like peptide‐1 (GLP‐1) secretion 15 min after oral glucose administration was significantly lower in SUC‐fed mice than in ST‐fed or control mice. Hepatic glucokinase (GCK) activity was significantly reduced in SUC‐fed mice. During the OGTT, the accumulation of glycogen in the liver was suppressed in SUC‐fed mice in a time‐dependent manner. Conclusions:  These results indicate that mice that consume a moderate SUC show glucose intolerance with a reduction in hepatic GCK activity and impairment in GLP‐1 secretion. (J Diabetes Invest, doi: 10.1111/j.2040‐1124.2012.00208.x, 2012) Blackwell Publishing Ltd 2012-03-20 2012-10-18 /pmc/articles/PMC4019243/ /pubmed/24843603 http://dx.doi.org/10.1111/j.2040-1124.2012.00208.x Text en © 2012 Asian Association for the Study of Diabetes and Wiley Publishing Asia Pty Ltd
spellingShingle Articles
Sakamoto, Eriko
Seino, Yusuke
Fukami, Ayako
Mizutani, Naohiro
Tsunekawa, Shin
Ishikawa, Kota
Ogata, Hidetada
Uenishi, Eita
Kamiya, Hideki
Hamada, Yoji
Sato, Hiroyuki
Harada, Norio
Toyoda, Yukiyasu
Miwa, Ichitomo
Nakamura, Jiro
Inagaki, Nobuya
Oiso, Yutaka
Ozaki, Nobuaki
Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion
title Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion
title_full Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion
title_fullStr Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion
title_full_unstemmed Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion
title_short Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion
title_sort ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019243/
https://www.ncbi.nlm.nih.gov/pubmed/24843603
http://dx.doi.org/10.1111/j.2040-1124.2012.00208.x
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