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Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion
Aims/Introduction: Excessive intake of sucrose can cause severe health issues, such as diabetes mellitus. In animal studies, consumption of a high‐sucrose diet (SUC) has been shown to cause obesity, insulin resistance and glucose intolerance. However, several in vivo experiments have been carried o...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019243/ https://www.ncbi.nlm.nih.gov/pubmed/24843603 http://dx.doi.org/10.1111/j.2040-1124.2012.00208.x |
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author | Sakamoto, Eriko Seino, Yusuke Fukami, Ayako Mizutani, Naohiro Tsunekawa, Shin Ishikawa, Kota Ogata, Hidetada Uenishi, Eita Kamiya, Hideki Hamada, Yoji Sato, Hiroyuki Harada, Norio Toyoda, Yukiyasu Miwa, Ichitomo Nakamura, Jiro Inagaki, Nobuya Oiso, Yutaka Ozaki, Nobuaki |
author_facet | Sakamoto, Eriko Seino, Yusuke Fukami, Ayako Mizutani, Naohiro Tsunekawa, Shin Ishikawa, Kota Ogata, Hidetada Uenishi, Eita Kamiya, Hideki Hamada, Yoji Sato, Hiroyuki Harada, Norio Toyoda, Yukiyasu Miwa, Ichitomo Nakamura, Jiro Inagaki, Nobuya Oiso, Yutaka Ozaki, Nobuaki |
author_sort | Sakamoto, Eriko |
collection | PubMed |
description | Aims/Introduction: Excessive intake of sucrose can cause severe health issues, such as diabetes mellitus. In animal studies, consumption of a high‐sucrose diet (SUC) has been shown to cause obesity, insulin resistance and glucose intolerance. However, several in vivo experiments have been carried out using diets with much higher sucrose contents (50–70% of the total calories) than are typically ingested by humans. In the present study, we examined the effects of a moderate SUC on glucose metabolism and the underlying mechanism. Materials and Methods: C57BL/6J mice received a SUC (38.5% sucrose), a high‐starch diet (ST) or a control diet for 5 weeks. We assessed glucose tolerance, incretin secretion and liver glucose metabolism. Results: An oral glucose tolerance test (OGTT) showed that plasma glucose levels in the early phase were significantly higher in SUC‐fed mice than in ST‐fed or control mice, with no change in plasma insulin levels at any stage. SUC‐fed mice showed a significant improvement in insulin sensitivity. Glucagon‐like peptide‐1 (GLP‐1) secretion 15 min after oral glucose administration was significantly lower in SUC‐fed mice than in ST‐fed or control mice. Hepatic glucokinase (GCK) activity was significantly reduced in SUC‐fed mice. During the OGTT, the accumulation of glycogen in the liver was suppressed in SUC‐fed mice in a time‐dependent manner. Conclusions: These results indicate that mice that consume a moderate SUC show glucose intolerance with a reduction in hepatic GCK activity and impairment in GLP‐1 secretion. (J Diabetes Invest, doi: 10.1111/j.2040‐1124.2012.00208.x, 2012) |
format | Online Article Text |
id | pubmed-4019243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-40192432014-05-19 Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion Sakamoto, Eriko Seino, Yusuke Fukami, Ayako Mizutani, Naohiro Tsunekawa, Shin Ishikawa, Kota Ogata, Hidetada Uenishi, Eita Kamiya, Hideki Hamada, Yoji Sato, Hiroyuki Harada, Norio Toyoda, Yukiyasu Miwa, Ichitomo Nakamura, Jiro Inagaki, Nobuya Oiso, Yutaka Ozaki, Nobuaki J Diabetes Investig Articles Aims/Introduction: Excessive intake of sucrose can cause severe health issues, such as diabetes mellitus. In animal studies, consumption of a high‐sucrose diet (SUC) has been shown to cause obesity, insulin resistance and glucose intolerance. However, several in vivo experiments have been carried out using diets with much higher sucrose contents (50–70% of the total calories) than are typically ingested by humans. In the present study, we examined the effects of a moderate SUC on glucose metabolism and the underlying mechanism. Materials and Methods: C57BL/6J mice received a SUC (38.5% sucrose), a high‐starch diet (ST) or a control diet for 5 weeks. We assessed glucose tolerance, incretin secretion and liver glucose metabolism. Results: An oral glucose tolerance test (OGTT) showed that plasma glucose levels in the early phase were significantly higher in SUC‐fed mice than in ST‐fed or control mice, with no change in plasma insulin levels at any stage. SUC‐fed mice showed a significant improvement in insulin sensitivity. Glucagon‐like peptide‐1 (GLP‐1) secretion 15 min after oral glucose administration was significantly lower in SUC‐fed mice than in ST‐fed or control mice. Hepatic glucokinase (GCK) activity was significantly reduced in SUC‐fed mice. During the OGTT, the accumulation of glycogen in the liver was suppressed in SUC‐fed mice in a time‐dependent manner. Conclusions: These results indicate that mice that consume a moderate SUC show glucose intolerance with a reduction in hepatic GCK activity and impairment in GLP‐1 secretion. (J Diabetes Invest, doi: 10.1111/j.2040‐1124.2012.00208.x, 2012) Blackwell Publishing Ltd 2012-03-20 2012-10-18 /pmc/articles/PMC4019243/ /pubmed/24843603 http://dx.doi.org/10.1111/j.2040-1124.2012.00208.x Text en © 2012 Asian Association for the Study of Diabetes and Wiley Publishing Asia Pty Ltd |
spellingShingle | Articles Sakamoto, Eriko Seino, Yusuke Fukami, Ayako Mizutani, Naohiro Tsunekawa, Shin Ishikawa, Kota Ogata, Hidetada Uenishi, Eita Kamiya, Hideki Hamada, Yoji Sato, Hiroyuki Harada, Norio Toyoda, Yukiyasu Miwa, Ichitomo Nakamura, Jiro Inagaki, Nobuya Oiso, Yutaka Ozaki, Nobuaki Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion |
title | Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion |
title_full | Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion |
title_fullStr | Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion |
title_full_unstemmed | Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion |
title_short | Ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion |
title_sort | ingestion of a moderate high‐sucrose diet results in glucose intolerance with reduced liver glucokinase activity and impaired glucagon‐like peptide‐1 secretion |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019243/ https://www.ncbi.nlm.nih.gov/pubmed/24843603 http://dx.doi.org/10.1111/j.2040-1124.2012.00208.x |
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